Liz, I am curious, why do you not use the recycled xylene prior to the paraffin step in the processor? And why not for cleaning?
I understand not recycling the cleaning alcohol or xylene, or even the first xylene on the stainer as they have too many contaminants. Nirmala, With regards to getting a lower quality of your alcohol, do you check the water content prior to recycling? We have found that it's not worth attempting to recycle any graded alcohols below 95% (that is to say, 70% or 80%). There is a "dilute" alcohol setting that can be used, but you will need the CBG folks to tell you how to access that one. It's there though! Michelle -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, May 04, 2010 11:44 AM To: sris...@mail.holyname.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CBG BIOTECH RECYCLER Nirmala We have the CBG recycler also. We initially started recycling both alcohol and xylene, but we now only recycle alcohol. We ended up with so much recycled xylene that we could not use. Also you can not use the recycled xylene on the last xylene station in the tissue processor, or for cleaning. It just did not work out for us for the xylene. We rotate our xylenes and alcohols weekly so we are always putting on one fresh absolute and one fresh xylene, there was never a place for us to use the recycled xylene, so we ended up with lots of it. If you change your entire tissue processor then you could put a recycled xylene in place of your first xylene and then use fresh xylene for the last station. We do recycle the alcohol. We get about 95% alcohol out of the recycler - we test it for percentage of alcohol via a hydrometer and for contamination with xylene by adding water to a small portion of it. We only use it for 95% or less so we make up our 50%, 70% and 80% alcohol from the recycled alcohol, these solutions do go on our tissue processor and in the staining set up and they seem to work just fine. To be honest we do not keep track of how many times the alcohol has been recycled we just keep recycling it. As for disposal we have a really cool flammable cabinet that houses a 55 gallon drum, the drum is on rollers so it can be moved easily. All of our waste goes in there its picked up whenever it is full. We use Source/AET Environmental to dispose of all our liquid waste. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sris...@mail.holyname.org Sent: Tuesday, May 04, 2010 9:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CBG BIOTECH RECYCLER Hi All, Needs some information on waste of xylene and alcohol. We are currently using a CBG recycler in our lab. We have been told that the clean alcohols and xylenes should not go in the recycler since it contains paraffin. Also the observation is, the alcohols put in the machine is percentage wise less than what is put in. In other words the recycled alcohols are used as 95% or less. Our cytology department does not used the recycled alcohols or xylenes since they claim to have staining issues. Does any one out there has developed a standard of howmany times the alcohols and xylene can be recycled? Do you have a company pick up your waste after a couple of recycles. Who helps out with the waste disposal and are they poured into 55 gallon drums and hauled away? Any assistance will be appreciated. Thanks Nirmala Srishan Holy Name Medical Center Teaneck NJ 07666 Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. 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