test Maria V. De Guzman| Histology Technician I
Main 908.947.1100 Fax 908.947.1085 Direct 908.947.1482 Email mdeguz...@lifecell.com Mobile 732.688.1386 www.Lifecell.com LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Tuesday, May 04, 2010 1:46 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 78, Issue 4 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Region II Symposium (Barone, Carol ) 2. Switching Back to Formalin (Jones, Laura) 3. RE: Switching Back to Formalin (Bauer, Karen L.) 4. RE: Switching Back to Formalin (WILLIAM DESALVO) 5. ? about a Leica RM2155 microtome (jstaruk) 6. mouse kidney frozen sectioning (Andrew Burgeson) 7. c1fe5960057c084ca389ce97779062904ebd0...@tcdmsg01.ad.texaschildrenshospital.org (Andrew Burgeson) 8. Re: ? about a Leica RM2155 microtome (Jack Ratliff) 9. Trichrome Help (Drew Meyer) 10. RE: Trichrome Help (Monfils, Paul) 11. Re: mouse kidney frozen sectioning (Michelle MacVeigh-Aloni) 12. Re: ? about a Leica RM2155 microtome (Pat Laurie) 13. specimen disposal (gerv...@aol.com) 14. Re: ? about a Leica RM2155 microtome (mariann.mailh...@leica-microsystems.com) 15. Formalin and Kylen recycling (mbbarra) 16. waterbath residue (path lab) 17. CBG BIOTECH RECYCLER (sris...@mail.holyname.org) 18. grossing qualifications (Donna M. Nolan) 19. RE: waterbath residue (Mahoney,Janice A) 20. RE: CBG BIOTECH RECYCLER (Liz Chlipala) 21. Re: waterbath residue (Lynette Pavelich) 22. Re: waterbath residue (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Mon, 3 May 2010 13:29:28 -0400 From: "Barone, Carol " <cbar...@nemours.org> Subject: [Histonet] Region II Symposium To: <histonet@lists.utsouthwestern.edu> Message-ID: <37e4bac017f57141af64faa5aeb04ce8033a7...@wlmmsx01.nemours.org> Content-Type: text/plain; charset="us-ascii" Histonetter's: For anyone planning to attend the Region II Sympoium....I wanted to give you a quick reminder that Monday May 10th, 2010 is the final day to get the discounted room rate at the Clarion Hotel for the Region II.. If you need information on the meeting you can find it through the NJ Society or any state society in the region ( DE, MD, PA, NJ, VA, W.VA amd District of Columbia)...and also NSH. The vendors attending will be many, and this will present a rare opportunity to get thos needed CEUs and network as well. Check out the program...make your selections...but, hurry on those discounted room rates....time is running out for that one! As the Region Director, I look forward to touching base with you all on the questions and suggestions you have for our Region! See you soon! Carol Barone ------------------------------ Message: 2 Date: Mon, 3 May 2010 14:15:45 -0400 From: "Jones, Laura" <lpjo...@srhs-pa.org> Subject: [Histonet] Switching Back to Formalin To: "Histonet (E-mail)" <Histonet@lists.utsouthwestern.edu> Message-ID: <4ae8039aea096143b965cbc6d0921668022ca80...@exch2007.srhs-pa.org> Content-Type: text/plain; charset="iso-8859-1" After years of working to get away from the use of Formalin in our lab, it seems as though we are going to be bringing it back. I'm seeking advice concerning what everyone is using to neutralize their formalin prior to disposal. Any recommendations welcome! Thanks in advance! Laura ------------------------------ Message: 3 Date: Mon, 3 May 2010 13:34:35 -0500 From: "Bauer, Karen L." <bauer.ka...@mayo.edu> Subject: RE: [Histonet] Switching Back to Formalin To: "Jones, Laura" <lpjo...@srhs-pa.org>, "Histonet (E-mail)" <Histonet@lists.utsouthwestern.edu> Message-ID: <53fc421cc200c5429929ede6c3676f30e35...@msgebe34> Content-Type: text/plain; charset="us-ascii" Don't dispose of it... Recycle it. Creative Waste Solutions has a nice "Green" recycler for smaller labs, but we're using the BR Instrument for our lab. I never imagined that we would recycle our formalin, but when we tried out the BR, I was very impressed. We've saved so much by not having to constantly purchase formalin and our surgery department has quit ordering it as well. Since we have much more recycled than we can use, surgery now gets their formalin from us. If you can't get a recycler, we used to use D'Formalizer from Surgipath to neutralize our formalin. Good luck, Karen Karen L. Bauer HT/HTL (ASCP) Histology Section Chief Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System 715-838-3205 bauer.ka...@mayo.edu -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Monday, May 03, 2010 1:16 PM To: Histonet (E-mail) Subject: [Histonet] Switching Back to Formalin After years of working to get away from the use of Formalin in our lab, it seems as though we are going to be bringing it back. I'm seeking advice concerning what everyone is using to neutralize their formalin prior to disposal. Any recommendations welcome! Thanks in advance! Laura _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Mon, 3 May 2010 13:06:40 -0600 From: WILLIAM DESALVO <wdesalvo....@hotmail.com> Subject: RE: [Histonet] Switching Back to Formalin To: <bauer.ka...@mayo.edu>, <lpjo...@srhs-pa.org>, histonet <histonet@lists.utsouthwestern.edu> Message-ID: <blu103-w27523d9eb5481df137469c91...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" I suggest Creative Waste Solutions for your Formalin recycling (actually refilering). We are a large volume lab and have been using multiple sytems (3 gallon to 20 gallon) for 5+ yrs without issue. By refiltering, you can reduce your purchase of new solution by at least 70%. There is no end point for re-use and there is no fish ordor as you get from heat distilation. Very cost effective and very user friendly. William DeSalvo, B.S., HTL(ASCP) > Date: Mon, 3 May 2010 13:34:35 -0500 > From: bauer.ka...@mayo.edu > To: lpjo...@srhs-pa.org; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Switching Back to Formalin > CC: > > Don't dispose of it... Recycle it. Creative Waste Solutions has a nice > "Green" recycler for smaller labs, but we're using the BR Instrument for > our lab. I never imagined that we would recycle our formalin, but when > we tried out the BR, I was very impressed. We've saved so much by not > having to constantly purchase formalin and our surgery department has > quit ordering it as well. Since we have much more recycled than we can > use, surgery now gets their formalin from us. > > If you can't get a recycler, we used to use D'Formalizer from Surgipath > to neutralize our formalin. > > Good luck, > > Karen > > Karen L. Bauer HT/HTL (ASCP) > Histology Section Chief > Department of Pathology - Luther Hospital > Luther Midelfort - Mayo Health System > 715-838-3205 > bauer.ka...@mayo.edu > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jones, > Laura > Sent: Monday, May 03, 2010 1:16 PM > To: Histonet (E-mail) > Subject: [Histonet] Switching Back to Formalin > > After years of working to get away from the use of Formalin in our lab, > it seems as though we are going to be bringing it back. I'm seeking > advice concerning what everyone is using to neutralize their formalin > prior to disposal. Any recommendations welcome! Thanks in advance! > Laura > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5 ------------------------------ Message: 5 Date: Mon, 3 May 2010 15:30:36 -0400 From: "jstaruk" <jsta...@masshistology.com> Subject: [Histonet] ? about a Leica RM2155 microtome To: "'histonet'" <histonet@lists.utsouthwestern.edu> Message-ID: <214d2296efb74fbda1e06471374b2...@jimpc> Content-Type: text/plain; charset="us-ascii" Is anyone out there using this machine? I was offered one without the foot switch. I was told this microtome will only work with the foot switch and cannot be operated manually. Can someone confirm this for me? Thanks Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com ------------------------------ Message: 6 Date: Mon, 03 May 2010 15:31:31 -0400 From: "Andrew Burgeson" <nap...@siscom.net> Subject: [Histonet] mouse kidney frozen sectioning To: histonet@lists.utsouthwestern.edu Message-ID: <4bdf2493.18f.2fc0.192170...@siscom.net> Content-Type: text/plain; charset="iso-8859-1" Having trouble with freezing artifact in the form of tiny fissures or cracks in mouse kidney on frozen section. Tissue is paraformaldehyde fixed and infiltrated w 70% aqueous sucrose OCT solution. Anyone else seen this and know how to deal with it? Thx ------------------------------ Message: 7 Date: Mon, 03 May 2010 15:37:20 -0400 From: "Andrew Burgeson" <nap...@siscom.net> Subject: [Histonet] c1fe5960057c084ca389ce97779062904ebd0...@tcdmsg01.ad.texaschildrenshospital.org To: histonet@lists.utsouthwestern.edu Message-ID: <4bdf25f0.10a.329b.1625663...@siscom.net> Content-Type: text/plain; charset="iso-8859-1" What procedures do you need to know? ------------------------------ Message: 8 Date: Mon, 3 May 2010 14:42:06 -0500 From: Jack Ratliff <ratliffj...@hotmail.com> Subject: Re: [Histonet] ? about a Leica RM2155 microtome To: jstaruk <jsta...@masshistology.com> Cc: histonet <histonet@lists.utsouthwestern.edu> Message-ID: <blu0-smtp7508b9e2fb494abc7e1cbae...@phx.gbl> Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes This is not true. You have to press the two blue buttons at the same time on the control panel to engage the motor. One button says run stop and the other says run enable. Remember that if you are in continuous mode you have to press the run stop button to stop the motor. Jack On May 3, 2010, at 2:30 PM, "jstaruk" <jsta...@masshistology.com> wrote: > Is anyone out there using this machine? I was offered one without > the foot > switch. I was told this microtome will only work with the foot > switch and > cannot be operated manually. Can someone confirm this for me? > > Thanks > > Jim > > _______________________ > James E. Staruk HT(ASCP) > www.masshistology.com > www.nehorselabs.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 9 Date: Mon, 3 May 2010 16:45:30 -0400 From: Drew Meyer <41dm...@gmail.com> Subject: [Histonet] Trichrome Help To: histonet@lists.utsouthwestern.edu Message-ID: <q2tebcbe38a1005031345u2694c215g118c2c677a990...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 I'm trying to troubleshoot a problem we had today with a Trichrome stain and I was wondering if anyone out there could help. We do Gomori Blue Collagen Trichrome Stain... 10 minutes in Weigert's Hematoxylin and then 15 min in the Gomori Blue Collagen stain followed by a quick change through acetic acid, alcohol and xylene. When it's all done, there is this weird "film" that is present over the entire surface of the slide... almost like the thin gray film that you sometimes see when you leave slides too long in the silver solution for a GMS. Anyways, I'm not sure what's causing it and putting the slide in xylene or alcohol for a long time doesn't get rid of it. It can easily be wiped off, but obviously we can't wipe the section off. If anyone could help me narrow down what step might be causing this, I'd appreciate it! Thanks, Drew ------------------------------ Message: 10 Date: Mon, 3 May 2010 18:22:22 -0400 From: "Monfils, Paul" <pmonf...@lifespan.org> Subject: RE: [Histonet] Trichrome Help To: <histonet@lists.utsouthwestern.edu> Message-ID: <4ebff65383b74d49995298c4976d1d5e06942...@lsriexch1.lsmaster.lifespan.org> Content-Type: text/plain; charset="us-ascii" Hi Drew, I don't know precisely what causes this, but I have seen it happen when using a freshly made batch of the dye solution. Also, a very fine whitish precipitate often collects in the bottom of the bottle in which the dye solution is stored, requiring filtration every day before using the solution - but only when the solution is fresh. Once the solution is a couple of weeks old, both problems disappear. Wish I had a better explanation. Paul ------------------------------ Message: 11 Date: Mon, 3 May 2010 15:43:55 -0700 From: "Michelle MacVeigh-Aloni" <macve...@usc.edu> Subject: Re: [Histonet] mouse kidney frozen sectioning To: <histonet@lists.utsouthwestern.edu> Message-ID: <4a84d2e9db3c4245a40e27531abd2...@dfs66dd1> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Hi Andrew, You must freeze fast to avoid the needle like crystals forming from the water during freezing. Best - freeze in OCT in liquid Nitrogen. It gets worse if you keep changing the temp of the block. For example keep at -80 then bring to -20 cut than bring the temp down to remove the block from the chuck and put back at -80. Few of those will make your tissue hard to even recognize. Good luck Michelle HTL USC Keck School of Medicine LA, CA ----- Original Message ----- From: "Andrew Burgeson" <nap...@siscom.net> To: <histonet@lists.utsouthwestern.edu> Sent: Monday, May 03, 2010 12:31 PM Subject: [Histonet] mouse kidney frozen sectioning > Having trouble with freezing artifact in the form of tiny > fissures or cracks in mouse kidney on frozen section. > > Tissue is paraformaldehyde fixed and infiltrated w 70% > aqueous sucrose OCT solution. > > Anyone else seen this and know how to deal with it? > > Thx > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 12 Date: Mon, 3 May 2010 16:02:07 -0700 From: Pat Laurie <foreig...@gmail.com> Subject: Re: [Histonet] ? about a Leica RM2155 microtome To: jstaruk <jsta...@masshistology.com> Cc: histonet <histonet@lists.utsouthwestern.edu> Message-ID: <g2qbdfbc2371005031602rd707ae1cu6b95ad38bb511...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 The RM 2155 does need a foot pedal to run. We've had ours where the connection wasn't secure, it would stop the microtome when it wasn't enabled. My techs use it as a manual mictotome though. The foot pedal just sits behind the micotome. The next generation RM 2255 and RM 2265 don't need the foot pedal to run. On Mon, May 3, 2010 at 12:30 PM, jstaruk <jsta...@masshistology.com> wrote: > Is anyone out there using this machine? I was offered one without the foot > switch. I was told this microtome will only work with the foot switch and > cannot be operated manually. Can someone confirm this for me? > > Thanks > > Jim > > _______________________ > James E. Staruk HT(ASCP) > www.masshistology.com > www.nehorselabs.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plau...@cellnetix.com ------------------------------ Message: 13 Date: Mon, 3 May 2010 22:53:49 EDT From: gerv...@aol.com Subject: [Histonet] specimen disposal To: Histonet@lists.utsouthwestern.edu, jason.burr...@crl.com, mdo...@siumed.edu Message-ID: <42ee4.164f7f94.3910e...@aol.com> Content-Type: text/plain; charset="US-ASCII" Hi, can you tell me the correct/safe way to dispose of the formalin fixed specimens? The fixed specimens should be considered hazardous waste, but the formalin is a chemical hazard. I think having biohazard waste company that burns these specimens with formalin . According to MSDS we should be able to burn the formalin and safely dispose of it that way. The large volumes of formalin off the processors is neutralized before disposal. But we are trying to avoid the labor intensive, hazardous job of emptying each specimen container. That is hazardous exposure since we have no work area to do this in safely. So, to finally get to the point, why can we not throw all the specimens in formalin into biohazard waste bags and containers? Pearl "Dance like no one is watching" ------------------------------ Message: 14 Date: Tue, 4 May 2010 08:09:45 -0500 From: mariann.mailh...@leica-microsystems.com Subject: Re: [Histonet] ? about a Leica RM2155 microtome To: Pat Laurie <foreig...@gmail.com> Cc: histonet <histonet@lists.utsouthwestern.edu>, jstaruk <jsta...@masshistology.com>, histonet-boun...@lists.utsouthwestern.edu Message-ID: <of8461a010.7225974f-on86257719.004814fa-86257719.00484...@leica-microsystems.com> Content-Type: text/plain; charset=US-ASCII Hi All I thought I would respond to the email. If you don't have a foot switch you can place a dummy plug in the back where the foot pedal goes. You would then have to get a hand pad to run the instrument. If you have questions you are welcome to give me a call. Kind Regards Mari Ann Mailhiot BA HT ASCP Application Specialist/Trainer Leica Microsystems Biosystems Division Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailh...@leica-microsystems.com www.leica-microsystems.com Pat Laurie <foreig...@gmail. com> To Sent by: jstaruk <jsta...@masshistology.com> histonet-bounces@ cc lists.utsouthwest histonet ern.edu <histonet@lists.utsouthwestern.edu> Subject Re: [Histonet] ? about a Leica 05/03/2010 06:02 RM2155 microtome PM The RM 2155 does need a foot pedal to run. We've had ours where the connection wasn't secure, it would stop the microtome when it wasn't enabled. My techs use it as a manual mictotome though. The foot pedal just sits behind the micotome. The next generation RM 2255 and RM 2265 don't need the foot pedal to run. On Mon, May 3, 2010 at 12:30 PM, jstaruk <jsta...@masshistology.com> wrote: > Is anyone out there using this machine? I was offered one without the foot > switch. I was told this microtome will only work with the foot switch and > cannot be operated manually. Can someone confirm this for me? > > Thanks > > Jim > > _______________________ > James E. Staruk HT(ASCP) > www.masshistology.com > www.nehorselabs.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plau...@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ ------------------------------ Message: 15 Date: Tue, 4 May 2010 10:29:59 -0300 From: mbbarra <mbba...@uol.com.br> Subject: [Histonet] Formalin and Kylen recycling To: histonet@lists.utsouthwestern.edu Message-ID: <4be02157b6ad9_385fdbc1670...@weasel10.tmail> Content-Type: text/plain; charset="utf-8" Some help Has anyone else use use BP instruments for? recycling. I need some information about the subject. Marinez Barra Laboratory of Pathology ------------------------------ Message: 16 Date: Tue, 4 May 2010 10:56:59 -0400 From: path lab <pathology.histol...@gmail.com> Subject: [Histonet] waterbath residue To: histonet@lists.utsouthwestern.edu Message-ID: <l2x73841641005040756g4e53067fidebc997d4f820...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Our waterbaths leave a good amount of black residue on our paper towels when we dump the water and wipe them out at the end of the day. Is anyone else having this problem? It appears that the finish is gradually wearing off. Could this be the cause of the artifact ( black specs appear to be on top of the tissue ) that we occassionally see? Thank you in advance for your replies. ------------------------------ Message: 17 Date: Tue, 4 May 2010 08:05:25 -0700 From: sris...@mail.holyname.org Subject: [Histonet] CBG BIOTECH RECYCLER To: histonet@lists.utsouthwestern.edu Message-ID: <of91fdd489.fb0973b9-on85257719.00520504-88257719.0062e...@holyname.org> Content-Type: text/plain; charset="US-ASCII" Hi All, Needs some information on waste of xylene and alcohol. We are currently using a CBG recycler in our lab. We have been told that the clean alcohols and xylenes should not go in the recycler since it contains paraffin. Also the observation is, the alcohols put in the machine is percentage wise less than what is put in. In other words the recycled alcohols are used as 95% or less. Our cytology department does not used the recycled alcohols or xylenes since they claim to have staining issues. Does any one out there has developed a standard of howmany times the alcohols and xylene can be recycled? Do you have a company pick up your waste after a couple of recycles. Who helps out with the waste disposal and are they poured into 55 gallon drums and hauled away? Any assistance will be appreciated. Thanks Nirmala Srishan Holy Name Medical Center Teaneck NJ 07666 Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. ------------------------------ Message: 18 Date: Tue, 4 May 2010 11:12:46 -0400 From: "Donna M. Nolan" <dno...@evanhospital.com> Subject: [Histonet] grossing qualifications To: Histonet <histonet@lists.utsouthwestern.edu> Message-ID: <d62a58fa76a99248aea53fb48ca559610b38180...@evanxsmb1.evanhospital.net> Content-Type: text/plain; charset="iso-8859-1" I was asked to get opinions about grossing personnel. We have a tech with an Associate of Science in Medical Assistant with 8 credits in Anatomy and Physiology, 4 credits in Microbiology, 3 credits in Medical Terminology, and 16 credits in Medical Assistant Theory and Practicum. Would you consider her qualified to gross specimens? Thanks, Donna This message has been scanned for malware by Websense. www.websense.com ------------------------------ Message: 19 Date: Tue, 4 May 2010 10:43:30 -0500 From: "Mahoney,Janice A" <janice.maho...@alegent.org> Subject: RE: [Histonet] waterbath residue To: 'path lab' <pathology.histol...@gmail.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <8f0ee4144e8e2f4ca1f6b051a2e5bfeea7c4a...@exchmbc2.ad.ah.local> Content-Type: text/plain; charset="us-ascii" Are you using pencils to mark your slides? Jan Omaha -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of path lab Sent: Tuesday, May 04, 2010 9:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] waterbath residue Our waterbaths leave a good amount of black residue on our paper towels when we dump the water and wipe them out at the end of the day. Is anyone else having this problem? It appears that the finish is gradually wearing off. Could this be the cause of the artifact ( black specs appear to be on top of the tissue ) that we occassionally see? Thank you in advance for your replies. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ------------------------------ Message: 20 Date: Tue, 4 May 2010 09:43:58 -0600 From: "Liz Chlipala" <l...@premierlab.com> Subject: RE: [Histonet] CBG BIOTECH RECYCLER To: <sris...@mail.holyname.org>, <histonet@lists.utsouthwestern.edu> Message-ID: <ee33be5c905a3046a7ff8f58a64c8e4b101...@server.premierlab.local> Content-Type: text/plain; charset="US-ASCII" Nirmala We have the CBG recycler also. We initially started recycling both alcohol and xylene, but we now only recycle alcohol. We ended up with so much recycled xylene that we could not use. Also you can not use the recycled xylene on the last xylene station in the tissue processor, or for cleaning. It just did not work out for us for the xylene. We rotate our xylenes and alcohols weekly so we are always putting on one fresh absolute and one fresh xylene, there was never a place for us to use the recycled xylene, so we ended up with lots of it. If you change your entire tissue processor then you could put a recycled xylene in place of your first xylene and then use fresh xylene for the last station. We do recycle the alcohol. We get about 95% alcohol out of the recycler - we test it for percentage of alcohol via a hydrometer and for contamination with xylene by adding water to a small portion of it. We only use it for 95% or less so we make up our 50%, 70% and 80% alcohol from the recycled alcohol, these solutions do go on our tissue processor and in the staining set up and they seem to work just fine. To be honest we do not keep track of how many times the alcohol has been recycled we just keep recycling it. As for disposal we have a really cool flammable cabinet that houses a 55 gallon drum, the drum is on rollers so it can be moved easily. All of our waste goes in there its picked up whenever it is full. We use Source/AET Environmental to dispose of all our liquid waste. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sris...@mail.holyname.org Sent: Tuesday, May 04, 2010 9:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CBG BIOTECH RECYCLER Hi All, Needs some information on waste of xylene and alcohol. We are currently using a CBG recycler in our lab. We have been told that the clean alcohols and xylenes should not go in the recycler since it contains paraffin. Also the observation is, the alcohols put in the machine is percentage wise less than what is put in. In other words the recycled alcohols are used as 95% or less. Our cytology department does not used the recycled alcohols or xylenes since they claim to have staining issues. Does any one out there has developed a standard of howmany times the alcohols and xylene can be recycled? Do you have a company pick up your waste after a couple of recycles. Who helps out with the waste disposal and are they poured into 55 gallon drums and hauled away? Any assistance will be appreciated. Thanks Nirmala Srishan Holy Name Medical Center Teaneck NJ 07666 Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Tue, 04 May 2010 11:56:58 -0400 From: "Lynette Pavelich" <lpave...@hurleymc.com> Subject: Re: [Histonet] waterbath residue To: "path lab" <pathology.histol...@gmail.com>, <histonet@lists.utsouthwestern.edu> Message-ID: <4be00b8a.59cd.00e...@hurleymc.com> Content-Type: text/plain; charset=US-ASCII Those black flecks could absolutely be from the waterbaths. I have yet to find a coated waterbath that does not eventually loose its lining. In frustration, I've had techs use a SOS pad to scrub off the lining to finally get rid of it. Waterbath still worked, just no nice teflon lining. I've tried returning them, but they all still went bad. Maybe next purchase, you could go the glass liner type. >>> path lab <pathology.histol...@gmail.com> 5/4/2010 10:56 AM >>> Our waterbaths leave a good amount of black residue on our paper towels when we dump the water and wipe them out at the end of the day. Is anyone else having this problem? It appears that the finish is gradually wearing off. Could this be the cause of the artifact ( black specs appear to be on top of the tissue ) that we occassionally see? Thank you in advance for your replies. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Tue, 4 May 2010 09:39:54 -0700 (PDT) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] waterbath residue To: path lab <pathology.histol...@gmail.com>, histonet@lists.utsouthwestern.edu, Lynette Pavelich <lpave...@hurleymc.com> Message-ID: <456410.74283...@web65712.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I used to glue a black X-ray film separating paraffinized sheet to the bottom of the water bath for a black background and changed it as needed. Ren? J. --- On Tue, 5/4/10, Lynette Pavelich <lpave...@hurleymc.com> wrote: From: Lynette Pavelich <lpave...@hurleymc.com> Subject: Re: [Histonet] waterbath residue To: "path lab" <pathology.histol...@gmail.com>, histonet@lists.utsouthwestern.edu Date: Tuesday, May 4, 2010, 11:56 AM Those black flecks could absolutely be from the waterbaths.? I have yet to find a coated waterbath that does not eventually loose its lining. In frustration, I've had techs use a SOS pad to scrub off the lining to finally get rid of it.? Waterbath still worked, just no nice teflon lining.? I've tried returning them, but they all still went bad.? Maybe next purchase, you could go the glass liner type. >>> path lab <pathology.histol...@gmail.com> 5/4/2010 10:56 AM >>> Our waterbaths leave a good amount of black residue on our paper towels when we dump the water and wipe them out at the end of the day.? Is anyone else having this problem?? It appears that the finish is gradually wearing off. Could this be the cause of the???artifact ( black specs appear to be on top of the tissue ) that we occassionally see?? Thank you in advance for your replies. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 78, Issue 4 *************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet