Eva, I would hold it in a buffer, i.e. Tris, PBS, etc. til you're ready to stain. You might also refrigerate the slide in buffer if its going to be overnight.
Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Eva Permaul Sent: Thursday, May 06, 2010 7:56 AM To: [email protected] Subject: [Histonet] dehydration of hydrated slide.... Good morning, I accidentally hydrated a FFPE slide that I can not stain today. It is in water. I did not do antigen retrieval. What do I do? Can I dehydrate the slide again? Will I be able to stain it later if I do? Thanks, Eva Permaul Georgetown University _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
