Maria, I am with Terri on this, if there is silver in your tissue it should show up on the H&E as some deposit.
Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of DeGuzman, Maria Sent: Friday, May 07, 2010 6:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special stain to demonstrate Silver Hello Histoneters, Is there a special stain to detect silver in tissue? If there is please share the procedure. Thanks, Fanny Maria V. De Guzman| Histology Technician I Main 908.947.1100 Fax 908.947.1085 Direct 908.947.1482 Email mdeguz...@lifecell.com Mobile 732.688.1386 www.Lifecell.com LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Thursday, May 06, 2010 2:16 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 78, Issue 7 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. clo test (Tench, Bill) 2. IDEA FOR A NEW RECYCLER!!!! (Madary, Joseph) 3. RE: RE: Barcode and Tracking Information (Rae Staskiewicz) 4. CD 133 (Ingles Claire ) 5. Disposable blade holder - amateur microscopist looking for a cheap one! (Gordon Brown) 6. Disposable blade holder - amateur microscopist looking for a cheap one! (Gordon Brown) 7. NSH Region II Meeting-discount hotel rate deadline is coming up! (Goodwin, Diana) 8. dehydration of hydrated slide.... (Eva Permaul) 9. RE: dehydration of hydrated slide.... (Sebree Linda A) 10. RE: dehydration of hydrated slide.... (Mauger, Joanne) 11. B5 fixative (histot...@imagesbyhopper.com) 12. myocyte damage (Bartlett, Jeanine (CDC/OID/NCZVED)) 13. RE: Responses to IHC CAP Validation question (tonia.richm...@gracepathology.com) 14. Starting up new lab (Shaw, Sharon) 15. Formalin fixation time for breast specimens (Richard Cartun) 16. RE: Responses to IHC CAP Validation question (bsulli...@shorememorial.org) 17. Re: myocyte damage (Merced M Leiker) ---------------------------------------------------------------------- Message: 1 Date: Wed, 5 May 2010 13:57:20 -0700 From: "Tench, Bill" <bill.te...@pph.org> Subject: [Histonet] clo test To: histonet@lists.utsouthwestern.edu Message-ID: <2820431bf953bb4da3e9e1a5882265fd02863...@mail1.pph.local> Content-Type: text/plain; charset=us-ascii The Clo test is a clinical lab test. You need to go to that part of the CPT coding book (sorry I don't have it available). 88300 is an anatomic code (gross only, ie, it requires examination of a piece of tissue or foreign body) and is entirely inappropriate for this test. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, May 05, 2010 1:36 PM To: histonet@lists.utsouthwestern.edu Subject: [BULK] Histonet Digest, Vol 78, Issue 6 Hi everyone, I am looking to see what CPT code everyone is using for reading Clo Tests in the pathology department. I have heard of using 87081 but I am not sure if this is accurate as this is for culture and the CLO is biochemical reaction not a culture. Currently I have been using 88300 gross only. Any help would be appreciated. Thank you, Amy Farnan *************************************** mail2.pph.org made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- ------------------------------ Message: 2 Date: Wed, 5 May 2010 17:22:22 -0400 From: "Madary, Joseph" <mada...@medimmune.com> Subject: [Histonet] IDEA FOR A NEW RECYCLER!!!! To: <histonet@lists.utsouthwestern.edu> Message-ID: <29a3cb81288e6f4ba2c9b3c8015a9a1301a37...@md1ev002.medimmune.com> Content-Type: text/plain; charset="us-ascii" Some manufacturer should find a way where you can add all of the chemicals together in one container and when the recycler runs it spits out formalin in one, xylene/hemo-de/f83/ in another and alcohol in another all from one run. To opine on another histnet query from earlier I think both have their place. I use CBG for xylene, form, hemo de and alcohol using the hemo on the processor and depar, alcohol on processor for low grade alc, and xyle for all but the last xylen on the stainer using frsh for that. Creative waste is good and simple but the only thing is mixing old and new formalin can't be good long term. What I was thinking about doing was using creative waste gravimetric for the first run, and then after that use the CBG to redistill NBF for round 2, 3, 4 etc. Hey where am I getting all this money and space? Still like BR recycler too, just do not have one anymore, but liked it. All recycling is good as long as people know which ones to use. Still seems to be an issue for some people. I worked in a place where the techs thought you could throw everyting in one container and the rcycler would spit out clean alcohol, xylene and formalin from one collective run. Hey manufactures? Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. ------------------------------ Message: 3 Date: Wed, 5 May 2010 17:32:47 -0500 From: "Rae Staskiewicz" <raest...@grics.net> Subject: RE: [Histonet] RE: Barcode and Tracking Information To: "'Mahoney,Janice A'" <janice.maho...@alegent.org>, "'Maggie Allen'" <maggie.al...@nicewareintl.com>, <histonet@lists.utsouthwestern.edu> Cc: histonet-requ...@lists.utsouthwestern.edu, ":histonet-bounces"@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu Message-ID: <926351c712074d27b3ac517297df0...@your4105e587b6> Content-Type: text/plain; charset="us-ascii" Jan, Ditto! Ditto! Ditto! Couldn't have said it better myself! Rae Staskiewicz -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Wednesday, May 05, 2010 2:53 PM To: 'Maggie Allen'; histonet@lists.utsouthwestern.edu Cc: histonet-requ...@lists.utsouthwestern.edu; ":histonet-bounces"@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: [Histonet] RE: Barcode and Tracking Information Blake, I think I put in my comments about Ventana's Vantage when you first posted the question, but here goes again.. It is wonderful. Vantage is the best thing to come along in my lifetime as a histo tech! Wonderful for patient safety, easy for the techs to use and a manager's dream for all the data it can provide. Jan Mahoney Omaha, NE -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maggie Allen Sent: Wednesday, May 05, 2010 2:24 PM To: histonet@lists.utsouthwestern.edu Cc: histonet-boun...@lists.utsouthwestern.edu; ":histonet-bounces"@lists.utsouthwestern.edu; histonet-requ...@lists.utsouthwestern.edu Subject: [Histonet] RE: Barcode and Tracking Information Hello Blake, LabelClinic HTS is a tracking system for Histology artifacts within the Pathology workflow. The product is designed to provide improved quality control and traceability with the lab. It provides flexible and reliable labeling and tracking of histology requisitions, containers, specimens, blocks and slides. Using bar code technology, LabelClinic HTS provides an optimized workflow that reduces errors and significantly enhances patient safety. It can be used stand alone, or integrated into an LIS / AP software system. Benefits: * Flexible lab configuration for custom lab workflow * Reduce errors and increase efficiency * Just-in-time reporting of artifact locations or last know location * Alerting for sub-process time violations and missing artifacts * Deploy to Desktop PC's or mobile hand held's * Configurable workflow * Supports color selection for slide and cassette printers I would be happy to set up an online WebEx demo of the system for anyone who may be interested. Thank you! Maggie Allen Healthcare Business Development Manager Niceware International, LLC 200 South Executive Drive Suite 200 Brookfield, Wisconsin 53005 Tel (810) 629-3930 Cell (215) 200-0268 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Wed, 5 May 2010 22:15:03 -0500 From: "Ingles Claire " <cing...@uwhealth.org> Subject: [Histonet] CD 133 To: <histonet@lists.utsouthwestern.edu> Message-ID: <f2f030053f9b7345831bed293a6d57e103a1a...@uwhc-mail01.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="iso-8859-1" Thought I'd try again... Anyone know where I can send some slides for a CD133? Claire ------------------------------ Message: 5 Date: Thu, 6 May 2010 09:16:36 +0100 From: "Gordon Brown" <gor...@10db.co.uk> Subject: [Histonet] Disposable blade holder - amateur microscopist looking for a cheap one! To: <histonet@lists.utsouthwestern.edu> Message-ID: <piepjogjhcpiepjkihpceeokebaa.gor...@10db.co.uk> Content-Type: text/plain; charset="iso-8859-1" I dabble in microscopy - well I say I dabble, my wife says otherwise! - and I have a reasonably well equipped home lab, which includes the ubiquitous Cambridge Rocker. I've had reasonably good results with plant tissue sections but sharpening the blades has always proved to be a pain and I tend to get mixed results. However, I've recently acquired a bunch of Accu Edge low profile blades at very low cost but I'm unable to source a holder at an affordable price. IS there anyone out there in professional histology land who has for sale a used, even rough condition holder suitable for these blades? You'd get the grateful thanks of both myself and my wife, who will be more than pleased to see me spend less time muttering about my poor sharpening skills! Many thanks Gordon (UK) ------------------------------ Message: 6 Date: Thu, 6 May 2010 09:36:41 +0100 From: "Gordon Brown" <gor...@10db.co.uk> Subject: [Histonet] Disposable blade holder - amateur microscopist looking for a cheap one! To: <histonet@lists.utsouthwestern.edu> Message-ID: <piepjogjhcpiepjkihpcieokebaa.gor...@10db.co.uk> Content-Type: text/plain; charset="iso-8859-1" Perhaps I should point out that despite the date in the UK, my name is genuinely as given, although my namesake may well be looking for a new hobby to fill in his spare time after today........... Gordon ------------------------------ Message: 7 Date: Thu, 6 May 2010 08:18:01 -0400 From: "Goodwin, Diana" <good...@labcorp.com> Subject: [Histonet] NSH Region II Meeting-discount hotel rate deadline is coming up! To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <eb4103c86804f442b0d76d16cbb09363d73b7d3...@rtwecs03.lca.labcorp.com> Content-Type: text/plain; charset="windows-1252" You don?t want to miss the upcoming Region II Meeting on June 10-12 in Atlantic City NJ! 25 speakers are presenting 30 different topics from wet workshops to short seminars. CEUs will be granted for all sessions. Over 30 vendors are participating in our Exhibit Area. Your registration fee includes free admission to the Vendor Exhibit, AM and PM Breaks, buffet lunch and Friday evening?s reception. The hotel room rate is reduced to $89/night, but you must reserve by Monday, May 10th! The mail-in registration deadline is May 20th. For more information, you can download a meeting brochure from the NSH website under state meetings at www.nsh.org/content/region-ii-meeting<http://www.nsh.org/content/region-ii-m eeting>. ----------------------------------------- This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyoffi...@labcorp.com or call (877) 23-HIPAA. ------------------------------ Message: 8 Date: Thu, 06 May 2010 08:56:01 -0400 From: Eva Permaul <e...@georgetown.edu> Subject: [Histonet] dehydration of hydrated slide.... To: histonet@lists.utsouthwestern.edu Message-ID: <4be2bc61.2040...@georgetown.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Good morning, I accidentally hydrated a FFPE slide that I can not stain today. It is in water. I did not do antigen retrieval. What do I do? Can I dehydrate the slide again? Will I be able to stain it later if I do? Thanks, Eva Permaul Georgetown University ------------------------------ Message: 9 Date: Thu, 6 May 2010 08:49:06 -0500 From: "Sebree Linda A" <lseb...@uwhealth.org> Subject: RE: [Histonet] dehydration of hydrated slide.... To: "Eva Permaul" <e...@georgetown.edu>, <histonet@lists.utsouthwestern.edu> Message-ID: <8c023b4ab999614ba4791baeb26e2738399...@uwhc-mail01.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="US-ASCII" Eva, I would hold it in a buffer, i.e. Tris, PBS, etc. til you're ready to stain. You might also refrigerate the slide in buffer if its going to be overnight. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eva Permaul Sent: Thursday, May 06, 2010 7:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] dehydration of hydrated slide.... Good morning, I accidentally hydrated a FFPE slide that I can not stain today. It is in water. I did not do antigen retrieval. What do I do? Can I dehydrate the slide again? Will I be able to stain it later if I do? Thanks, Eva Permaul Georgetown University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Thu, 6 May 2010 11:02:59 -0400 From: "Mauger, Joanne" <mau...@email.chop.edu> Subject: RE: [Histonet] dehydration of hydrated slide.... To: Sebree Linda A <lseb...@uwhealth.org>, Eva Permaul <e...@georgetown.edu>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <443f5b475a9bf647ab962e834884ebad2788770...@ex7ccrpw03v1.chop.edu> Content-Type: text/plain; charset="us-ascii" Eva, If you dehydrate it again to 100% or xylene, it is less likelt to fall off the slide. Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A [lseb...@uwhealth.org] Sent: Thursday, May 06, 2010 9:49 AM To: Eva Permaul; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] dehydration of hydrated slide.... Eva, I would hold it in a buffer, i.e. Tris, PBS, etc. til you're ready to stain. You might also refrigerate the slide in buffer if its going to be overnight. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eva Permaul Sent: Thursday, May 06, 2010 7:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] dehydration of hydrated slide.... Good morning, I accidentally hydrated a FFPE slide that I can not stain today. It is in water. I did not do antigen retrieval. What do I do? Can I dehydrate the slide again? Will I be able to stain it later if I do? Thanks, Eva Permaul Georgetown University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Thu, 6 May 2010 11:20:01 -0400 From: <histot...@imagesbyhopper.com> Subject: [Histonet] B5 fixative To: <histonet@lists.utsouthwestern.edu> Message-ID: <a00e24adafd4471dac50d9718c353...@hopperpc> Content-Type: text/plain; charset="US-ASCII" I was under the impression that B5, because of the mercury content, was outlawed for use Jan 1, 2005. But now I am not so sure! Can anyone tell me if there is a federal law regarding this? We no longer use B5, we use B+, but I know someone in OK who is using B5 (I am in FL). Is he breaking any laws by using it and/or should he be switched to an alternative like B+? Thanks! Michelle ------------------------------ Message: 12 Date: Thu, 6 May 2010 11:16:04 -0400 From: "Bartlett, Jeanine (CDC/OID/NCZVED)" <j...@cdc.gov> Subject: [Histonet] myocyte damage To: histonet <histonet@lists.utsouthwestern.edu> Message-ID: <68510b12184e45498eabd4cb6f3868fe01287...@lta3vs001.ees.hhs.gov> Content-Type: text/plain; charset=us-ascii Hello everyone, I am in need of a special stain or an IHC that will demonstrate myocyte damage in muscle tissue...esp. cardiac. Any help will be greatly appreciated. Thanks! Jeanine Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartl...@cdc.hhs.gov ------------------------------ Message: 13 Date: Thu, 6 May 2010 10:27:50 -0500 From: tonia.richm...@gracepathology.com Subject: RE: [Histonet] Responses to IHC CAP Validation question To: "McMahon, Loralee A" <loralee_mcma...@urmc.rochester.edu> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, "thisis...@aol.com" <thisis...@aol.com> Message-ID: <of2b2d84a8.322a1680-on8625771b.0054f24c-8625771b.0054f...@gracepathology.co m> Content-Type: text/plain; charset="UTF-8" If you are a brand new lab, how do you validate IHC if you are no yet receiving patient specimens? Can the validation be done on cont rol tissues only? Sincerely, Tonia Richmond, AS, HT (ASCP) Chief Operations Officer Grace Pathology PH: (501) 765-7367 Email: -----histonet-boun...@lists.utsouthwestern.edu wrote: ----- < To: "thisisann@ "histonet@lists.utsouthwestern.edu" < ;histonet@lists.utsouthwestern.edu> From: "McMahon, Loralee A" <Lo Sent by: histonet-boun...@lists.u Date: 04/28/2010 02:01PM Subject: RE: [Histonet] Re Any inspection that I have under case rule. Except for the Er/Pr//Her-2 cases. We also use a TMA to make our live contains known positives and known negatives. I cases i markers (SV- in a slide that w then you can say it has spe Any inspector that I have come across is usually understanding this. But I am sure that there are exceptions to this.........esp ecially if they are not familiar with immunohistochemistry. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong M Department of Surgical Pathology (585) 275-7210 ______________________ 5F__ From: histonet-boun...@lis [histonet-boun...@lists.utsouthwestern.edu] On Behalf thisis...@aol.com [thisis...@aol.com] Sent: Wednesday, April 28, 201 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] R The following is one respone 1. I asked CAP who told me that they do not currentl guideline on validating but that they recommend what is in t Quality Management In Anatomic Pathology, Promoting P Safety Through Systems Improvement and Error by Raouf E. Nakhl sold by CAP ! Chapter 8- That is what we follow. I. Get a new antib II. Once optimized you n positive (how many?) "a suffien III. Must also be run on cases expected to be negative. (how IV. In a situation where you cannot expect a lot of cases or such a case has never been presented in your lab, then you must say just (ex. some of the hormones we just use a pituitary) ___________________ ______________________ 5F__ Histonet mailing list Histonet@lists.utsouthwestern.edu ______________________ 5F__ ______________________ Histo Histonet@lists.utsouthwestern.edu [3]http://l References 1. 3D"mailto:tonia.richm...@gracepathology.com" 2. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 3. 3D"http://=/ ------------------------------ Message: 14 Date: Thu, 6 May 2010 11:42:40 -0400 From: "Shaw, Sharon" <shs...@wpi.edu> Subject: [Histonet] Starting up new lab To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <aa21da463c7b1143a22faf799668f05f547adc7...@exchangemail.admin.wpi.edu> Content-Type: text/plain; charset="us-ascii" I'm looking at starting up a new histology lab and need everything, I have a tight budget. Can anybody give me recommendations on where to buy refurbished equipment. Thanks Sharon ------------------------------ Message: 15 Date: Thu, 06 May 2010 11:57:43 -0400 From: "Richard Cartun" <rcar...@harthosp.org> Subject: [Histonet] Formalin fixation time for breast specimens To: "Histonet" <histonet@lists.utsouthwestern.edu> Message-ID: <4be2aeb7.7400.007...@harthosp.org> Content-Type: text/plain; charset=US-ASCII There has been a lot of discussion recently regarding recommendations for formalin fixation of breast specimens. If you are interested in this topic please read the following article published in the May 2010 issue of the American Journal of Clinical Pathology by Ibarra JA, et al., "Fixation time does not affect the expression of estrogen receptor". Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ------------------------------ Message: 16 Date: Thu, 6 May 2010 12:16:45 -0400 From: bsulli...@shorememorial.org Subject: RE: [Histonet] Responses to IHC CAP Validation question To: tonia.richm...@gracepathology.com Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, "McMahon, Loralee A" <loralee_mcma...@urmc.rochester.edu>, histonet-boun...@lists.utsouthwestern.edu, "thisis...@aol.com" <thisis...@aol.com> Message-ID: <of88a1fd4e.77204cc6-on8525771b.00591b88-8525771b.00599...@shorememorial.org > Content-Type: text/plain; charset=US-ASCII One important thing to remember is that you should make sure All material being used for validation is processed the same way. Will your control tissue be purchased or processed in your lab? Control tissue is used for validation but you should use tissue with various levels of positivity. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 tonia.richm...@gr acepathology.com Sent by: To histonet-bounces@ "McMahon, Loralee A" lists.utsouthwest <loralee_mcma...@urmc.rochester.edu ern.edu > cc "histonet@lists.utsouthwestern.edu" 05/06/2010 11:27 <histonet@lists.utsouthwestern.edu> AM , "thisis...@aol.com" <thisis...@aol.com> Subject RE: [Histonet] Responses to IHC CAP Validation question If you are a brand new lab, how do you validate IHC if you are no= t yet receiving patient specimens? Can the validation be done on cont rol tissues only? Sincerely, Tonia Richmond, AS, HT (ASCP) Chief Operations Officer = / Laboratory Grace Pathology PH: (501) 765-7367 Email: = [1]tonia.= richm...@gracepathology.com -----histonet-boun...@lists.utsouthwestern.edu wrote: ----- <= /FONT> To: "thisis...@= aol.com" <thisis...@aol.com>, "histonet@lists.utsouthwestern.edu" < ;histonet@lists.utsouthwestern.edu> From: "McMahon, Loralee A" <Lo= ralee_mcma...@urmc.rochester.edu> Sent by: histonet-boun...@lists.u= tsouthwestern.edu Date: 04/28/2010 02:01PM Subject: RE: [Histonet] Re= sponses to IHC CAP Validation question Any inspection that I have under= gone we have used the 25 to 30 case rule. Except for the Er/Pr//Her-2= . We use closer to 50 cases. We also use a TMA to make our live= s easier. The TMA contains known positives and known negatives. I= n cases of t-cell or b-cell markers or cytokeratins. 25 to 30 cases i= s easy. But when you are validated for more hard to find markers (SV-= 40) then fewer cases is acceptable. We always throw in a slide that w= e know will not stain for sv-40 like a tonsil - then you can say it has spe= cificity. Any inspector that I have come across is usually understanding= of this. But I am sure that there are exceptions to this.........esp ecially if they are not familiar with immunohistochemistry. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong M= emorial Hospital Department of Surgical Pathology (585) 275-7210 ______________________ 5F__= _______________ From: histonet-boun...@lis= ts.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf= Of thisis...@aol.com [thisis...@aol.com] Sent: Wednesday, April 28, 201= 0 2:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] R= esponses to IHC CAP Validation question The following is one respone= I rec'd: 1. I asked CAP who told me that they do not currentl= y have a guideline on validating but that they recommend what is in t= he following book: Quality Management In Anatomic Pathology, Promoting P= atient Safety Through Systems Improvement and Error by Raouf E. Nakhl= eh, MD & Patrick Fitzgibbons, MD editors sold by CAP ! Chapter 8-= Quality Management in IHC That is what we follow. I. Get a new antib= ody and optimize it with your positive control. II. Once optimized you n= eed to run it on cases expected to be positive (how many?) "a suffien= t size ..." III. Must also be run on cases expected to be negative. (how= many? IV. In a situation where you cannot expect a lot of cases or such a case has never been presented in your lab, then you must say just = that. (ex. some of the hormones we just use a pituitary) ___________________ ______________________ 5F__= ___ Histonet mailing list Histonet@lists.utsouthwestern.edu = [2]http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________ 5F__ ______________________ Histo= net mailing list Histonet@lists.utsouthwestern.edu [3]http://l= ists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:tonia.richm...@gracepathology.com" 2. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 3. 3D"http://=/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Thu, 06 May 2010 12:30:50 -0400 From: Merced M Leiker <lei...@buffalo.edu> Subject: Re: [Histonet] myocyte damage To: "Bartlett, Jeanine (CDC/OID/NCZVED)" <j...@cdc.gov>, histonet <histonet@lists.utsouthwestern.edu> Message-ID: <5afa0a6d83365a1f044d4...@cdywxp1931.ad.med.buffalo.edu> Content-Type: text/plain; charset=us-ascii; format=flowed Stain with cTnI and inspect for damage visually. Just a guess. Regards, Merced --On Thursday, May 06, 2010 11:16 AM -0400 "Bartlett, Jeanine (CDC/OID/NCZVED)" <j...@cdc.gov> wrote: > Hello everyone, > > I am in need of a special stain or an IHC that will demonstrate myocyte > damage in muscle tissue...esp. cardiac. > > Any help will be greatly appreciated. > > Thanks! > Jeanine Bartlett > Centers for Disease Control and Prevention > Infectious Diseases Pathology Branch > 1600 Clifton Road, MS/G-32 > 18/SB-114 > Atlanta, GA 30333 > (404) 639-3590 > jeanine.bartl...@cdc.hhs.gov > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 78, Issue 7 *************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet