Patsy is correct, there is a very good chance the retrieval is the issue. Another possible issue is the drying time. We find often times with tissue loss we can remedy that with extended drying times. If we get precut slides from an outside source, we dry them for 2 hours in a 58 degree oven to be cautious.
Kelsey Jones Technical Consultant 6600 Sierra College Blvd. Rocklin, CA 95677 (916) 746-8900 x8969 (916) 746-8989 (fax) www.cellmarque.com **Check out Cell Marque on Facebook! -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Patsy Ruegg Sent: Saturday, May 08, 2010 4:11 PM To: 'Joseph N. Pastore'; [email protected] Subject: RE: SPAM-LOW: [Histonet] Loss of tissue sections after antigenretrieval Joe, Did you buy an array that was not cut with the tape transfer system and put onto polymer coated slides? If they had done that your tissues would not fall off, not sure of what to do after the fact, but you could certainly try a more gentle antigen retrieval technique such as waterbath (not sure of what you are using as your heat source?) sounds like your retrieval method is too harsh. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Joseph N. Pastore Sent: Saturday, May 08, 2010 1:32 PM To: [email protected] Subject: SPAM-LOW: [Histonet] Loss of tissue sections after antigen retrieval Hi All, We have a problem. We are using a commercial tissue array and we find that the two most important tissues are lost during antigen retrieval. The tissues are samples of glioblastomas and are critical to our study. The antigen retrieval system we use is a proprietary 2 step system using a hi and lo pH. Both Ca chelators. Does anyone have suggestions about how we can prevent tissue loss???? _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
