Why not embed in resin (MMA) and take thicker sections and then grind/
polish them down? If you went this route, you could then use
flourescent labels and quantify mineral apposition rate and bone
formation rate. Let me know if you are interested. I can help you get
started and direct you to low cost equipment options.
Jack
On Apr 22, 2010, at 9:58 AM, "Connolly, Brett M" <brett_conno...@merck.com
> wrote:
A colleague is having trouble getting wrinkle-free sections of
decalcified, paraffin embedded femur.
Any tips??
Thanks,
Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
tel. 215-652-2501 fax. 215-993-6803
brett_conno...@merck.com
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