I'm not sure if that was clear looking back on it:
Incubate both primaries together.
Then incubate both secondaries together.
--On Thursday, May 13, 2010 2:46 PM -0400 Merced M Leiker
<lei...@buffalo.edu> wrote:
You could try incubating both together (primaries and then secondaries).
I have done this before with primaries of differing isotypes. I know
others have, too.
Regards,
Merced
--On Thursday, May 13, 2010 2:00 PM -0400 Igor Deyneko
<igor.deyn...@gmail.com> wrote:
Hello Everyone!
I'm planning to try some IF co-staining with 2 antibodies, one is a
rat-anti-mouse and the other one is rabbit anti-human on a xenograft,
each has an appropriate secondary, donkey anti rat and donkey
anti-rabbit, conjugated to 488 and 593. Can someone advise the best way
to perform such a procedure, I'm afraid the rules of sequential staining
might not work due to fluorophore instability with washes. if anyone has
performed such type of stain, i would appreciate any tips.
Thank you.
Igor Deyneko
Infinity Pharmaceuticals
Cambridge, MA 02139
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214 USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)
No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214 USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)
No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
