Hello, We have done a study and found that storage in small zip-loc bags at -20 is an inexpensive and convenient way to store slides. In fact the slides stored for 5 years at -20 stain better than freshly cut sections from the same blocks. This was also published recently by others. Dr Rimm at Yale does have the storage under nitrogen gas system that is available commercially.
Helen L. Fedor Tissue Microarray Lab, Manager Prostate Spore Lab, Manager Johns Hopkins University 600 N. Wolfe St, | Marburg Room 406 Baltimore, MD | 21287-7065 410.614.1660 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Saturday, May 15, 2010 9:36 AM To: SamuelPerry; histonet@lists.utsouthwestern.edu; TimMorken Subject: Re: [Histonet] RE: Prolonged FFPE slide and block storage Inexpensive long term anti oxidizing storage? Immerse them in mineral oil! René J. --- On Fri, 5/14/10, Morken, Tim <timothy.mor...@ucsfmedctr.org> wrote: From: Morken, Tim <timothy.mor...@ucsfmedctr.org> Subject: [Histonet] RE: Prolonged FFPE slide and block storage To: "Perry, Samuel" <samuel_pe...@dfci.harvard.edu>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Date: Friday, May 14, 2010, 5:05 PM I have a paper from Yale several years ago about their tissue array facility. They found that storage in nitrogen helped a lot. I will try to find it, but you may be able to google it faster. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Perry, Samuel Sent: Friday, May 14, 2010 1:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Prolonged FFPE slide and block storage Hi All, We have a growing need for storing slides. They need to be stored in a way that they won't become oxidized and loose their antigenicity, since we use them for IHC. Any suggestions for inexpensive methods for prolonged storage of FFPE slides and blocks is greatly appreciated. Thanks! Sam Perry Research Technician Dana-Farber Cancer Institute Boston, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet