Jim,
I must say that I have used Eukitt exclusively for over 13 years and
the only problem I have had with Eukitt is when I do not use enough
and especially when coverslipping thicker sections. It is xylenes
based so maybe you are experiencing excessive evaporation during
drying??? If you are diluting the Eukitt with xylenes so that it flows
better (not as viscious) and reduces the chance for air bubbles, this
might cause excessive shrinkage and/or the problems you are
experiencing.
Jack
On May 14, 2010, at 3:22 PM, "Herrick, James L. (Jim)" <herrick.ja...@mayo.edu
> wrote:
Hello everyone,
Hope you have all had a good week!!
Has anyone had any negative experiences using Eukitt as your mounting
media? We embed our specimens (human - iliac crest, animal - femurs,
tibias, vertebrae, etc.) in GMA and MMA and have noticed over a period
of time that a fairly large number of our slides are beginning to show
signs of the medium breaking up below the cover slip. It looks as if
there are large air pockets left behind. We are not yet sure why
this is
happening. Would any of you geniuses have a resolution for us or
know of
a better mounting media to use with plastic embedded specimens -
brightfield and fluorescence? Thanks for your expertise. It is very
much
appreciated.
Have a great weekend!!
Jim
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