Hi Sarah, I used to make a ton of cytospins. I would air-dry them, fix them in whatever (ethanol or acetone/ethanol), air-dry them again and then wrap the slides in foil, box them up and store @ -80C. The slides lasted for months!
When taking them out to use for staining, be sure to allow the wrapped slides to acclimate to RT before you use them. Happy staining! Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: "sgoe...@xbiotech.com" <sgoe...@xbiotech.com> To: histonet@lists.utsouthwestern.edu Sent: Tue, May 18, 2010 2:36:56 PM Subject: [Histonet] ICC slide storage So, again...I'm new to this ICC staining. I got a epindorf = tube of cells (and a PBMC), I made cytospins from them and then fixed them in acetone, then 100& alcohol, then rehydrated them in PBS. The f irst day I did the spins the slides looked great, you could definately see = the monocytes. Then the days that have followed the cells have become= lysed, and there is "gradoo" all over the place. I think that it pro= bably has something to do with the fact that the cells weren't fixed and po= pped. My question is can I fix the slides and then leave them in 4 de= grees fridge either in the alcohol or take them out in a slide box. I= can see not reconsituting them in the saline until ready to stain, but I t= hink I need to fix them and not just leave the fluid sitting in the fridge?= Ahh help!!! Thanks guys and gals =) Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotec= h USA Inc. 8201 East Riverside Dr. B= ldg 4 Suite 100 Austin, Texas = 78744 (512)386-5107 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet