Laurie I'm not aware of a particular question, but I would believe you would have to perform some validation steps for each antibody. I would approach it the same way you approach validating new lots of antisera. The CAP paper on standardization of IHC recommends 25 different samples when you initially validate an antibody - 10 samples that have high levels of target antigen, 10 intermediate to low levels and 5 negative. To revalidate new lots they recommend only 3 tissue samples - 1 high, 1 med to low, and 1 negative. Granted this only applies to routine markers. For prognostic markers such as ER/PR and Her2 then additional samples need to be tested - there are new guidelines for ER/ER out and the new recommendations for validation for ER/ER are briefly reviewed in the CAP Today April issue. Guidelines for validation of ER/PR will be published in the June issue of Archives of Pathology and Laboratory Medicine.
Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, May 19, 2010 2:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Validation (again) Can anyone tell me if there is a specific question on the CAP checklist that addresses revalidation of antibodies when starting up a new IHC stainer? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet