Actually if I could make a minor correction to your statement: propidium
iodide is excited by green light at 488nm but emits in the red portion of
the spectrum (620nm)...
--On Thursday, May 20, 2010 12:48 PM -0600 Liz Chlipala
<l...@premierlab.com> wrote:
I think that the propidium iodide will stain the nuclei or DNA, similar
to DAPI, so this mounting media will counterstain the nuclei and its
visible in the FITC wavelength (488), while if you conterstain with DAPI
it is excited with a different wavelength, therefore it might interfere
with the FITC portion of your stain.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com
Ship to Address:
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Longmont, Colorado 80504
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie
Colbert
Sent: Thursday, May 20, 2010 12:13 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Mounting Medium for Immunofluorescence
We normally coverslip our immunofluorescence slides with a permanent
aqueous mounting medium. By mistake, today they were coverslipped with
a mounting medium that we use for coverslipping our FISH slides. This
mounting medium is described as "Mounting medium for fluorescence, with
propidium iodide."
Will this make a difference when reading the slides??
Laurie Colbert
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Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214 USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)
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However, many electrons were severely inconvenienced.
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