My first question would be what are you using for a control? I know this sounds strange but I have, in the past, had a problem with commercially made ones. The AFB actually came away from the material and applied itself to the patient slide. Crazy huh? This caused a real mess until we figured it out and changed vendors. Also if you are doing them by hand you need to filter after each use. Hope this helps. There is a definate difference in appearance between a patient positive result and a loose organism. Hope this helps.
Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 <debra.or...@ucho spitals.edu> Sent by: To histonet-bounces@ <histonet@lists.utsouthwestern.edu> lists.utsouthwest cc ern.edu Subject [Histonet] possible AFB contaminant 05/20/2010 03:41 PM Please help, we have had an increase in demands for repeats on our AFB stain. Our pathologists have been noticing what they believe are "false positives" on our slides. We perform our AFBs on the Ventana Nexus and some are done by hand. Because of this problem, we have been doing side by side comparisons using the Nexus and hand stain. We still have complaints. We have made new reagents, changed kits, tested our DI water sytem and have different techs cutting using different waterbaths. Any advice or comments would be greatly appreciated. Thanks Debi ******************************************************************************** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Medical Center ******************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet