Albert: In histology there are only very few standards. That is why CAP has a check list when inspecting histolabs. If you are doing a "validation", get as many different tissue sections as you can. Stain them "as usual" (namely using the method you are trying to substitute). Stain identical sections with your method or instruyment. Label them just by numbers, and give them to the pathologist for a blind evaluation. They should answer just one questions: is either section within each pair better than the other (telling which one) or are they equally useful for diagnosis. The results analyze with Chi a square test where the "theoretical" frequency is P=0.50. As "simple" as that. René J.
--- On Fri, 6/4/10, Santiago, Albert <albert.santi...@uphs.upenn.edu> wrote: From: Santiago, Albert <albert.santi...@uphs.upenn.edu> Subject: [Histonet] H&E Stainer Validations To: histonet@lists.utsouthwestern.edu Date: Friday, June 4, 2010, 11:30 AM Hello fellow Histonetters, I'm going to Demo a new H&E stainer and I was wondering if anyone knows if there's a standard H&E Validation protocol or if it just varies from lab to lab? Thanks for your help.... :-) Albert Santiago, HT(ASCP) Laboratory Manager Dermatopathology 215-662-6759/6539-office 215-520-3797-BB 215-662-7520-fax We will be relocating, as of 6/14/2010 our lab will be located at 3020 Market St. Ste. 201 The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
