We use a modified Gomori's one step....with excellent results on frozen muscle. You can find the protocol in Muscle Pathology and Histochemistry, Harvey Sarnat. It is an excellent stain...and if you have a limited amount of tissue, it gives you the most information on a single slide....(and if the tissue was quickly... and properly frozen....will include fiber typing).
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Sunday, June 13, 2010 1:28 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 79, Issue 13 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Can you do Masson's trichrome on frozen sections (Robert Richmond) 2. Ken Marissael is out of the office (ken_mariss...@vwr.com) 3. Jaclyn Bhamornsiri is out of the office (jaclyn_bhamorns...@vwr.com) ---------------------------------------------------------------------- Message: 1 Date: Sat, 12 Jun 2010 13:59:58 -0400 From: Robert Richmond <rsrichm...@gmail.com> Subject: [Histonet] Re: Can you do Masson's trichrome on frozen sections To: histonet@lists.utsouthwestern.edu Message-ID: <aanlktinz1bqagomkhgjws3lwh1cqnvtjsehkkzh7u...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 The Engel-Cunningham variant of the Gomori trichrome stain was developed for skeletal muscle frozen sections, and is a fairly simple one-step trichrome stain once you get it working. I've never used it for any other tissue. I think it's commercially available in kit form. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 2 Date: Sat, 12 Jun 2010 14:01:13 -0400 From: ken_mariss...@vwr.com Subject: [Histonet] Ken Marissael is out of the office To: histonet@lists.utsouthwestern.edu Message-ID: <of94acc4c9.d004a782-on85257740.0062fd0b-85257740.0062f...@vwr.com> Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 06/12/2010 and will not return until 06/13/2010 ------------------------------ Message: 3 Date: Sat, 12 Jun 2010 14:01:14 -0400 From: jaclyn_bhamorns...@vwr.com Subject: [Histonet] Jaclyn Bhamornsiri is out of the office To: histonet@lists.utsouthwestern.edu Message-ID: <of0e1637b9.db60f492-on85257740.0062fd75-85257740.0062f...@vwr.com> Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 06/11/2010 and will not return until 06/12/2010 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 79, Issue 13 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
