Happy Friday! We recently purchased a cryojane for sectioning frozen human and rat cartilage. I am having some issues with my cartilage sections. I am using the 4X slides with 2 flashes. The sections look beautiful, until I put them into buffer to do staining. I am getting folds and the sections are lifting off during IHC staining. I am staining either by hand (on flat trays, old-fashioned style) or in the sequenza racks. Luckily, the cartilage is not not coming off the slides completely, so I have been able to get the data I need. I was wondering if anyone had any tips for me to prevent the folds or the lift-off.
Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
