We have success with Sandison Method, see below:
Sandison Method
Solutions:
95% ethanol 300ml
38% Formalin 10ml
Sodium carbonate 10g
Water 690ml
Method:
Leave tissues in solution overnight or until they become soft. Replace
one third of fluid with 95% ethanol, repeat until tissue becomes firm.
Reference:
"The histological examination of Mummified Tissue" Stain Techn
30:277-283, 1955
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Parsons,
Catherine
Sent: Thursday, 24 June 2010 1:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Help Resurrecting Dried Fixed Tissue
I am grasping at straws and hoping for ideas to salvage some formalin
fixed tissue specimens. I work in a research lab, and am quite new to
histology and this particular lab. A former student left formalin fixed
tissue specimens that were stored in 70% ethanol. Unfortunately, the
seal failed on a few of the vials, the ethanol evaporated, and the
tissue is a dried out disaster at the bottom of the vial.
Has anyone has any experience / luck rehydrating tissues? Really, any
information that I could possibly pull out of these specimens would be
welcome.
Cathy Parsons
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