For Malika I have the Leica Coverslipper and I use Statlab coverslips. They work great and almost no waste. Hope this helps you ... Audrey Toothman Parma Community General Hospital Parma Ohio
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Tuesday, June 29, 2010 1:24 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 79, Issue 38 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Canine bone sectioning trouble (Schneider,Lynda S) 2. RE: Coverslips (Malika Benatti) ---------------------------------------------------------------------- Message: 1 Date: Tue, 29 Jun 2010 12:17:58 -0400 From: "Schneider,Lynda S" <emly...@pathology.ufl.edu> Subject: [Histonet] Canine bone sectioning trouble To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <94a0bfce84cb0f4a84c50eac9b02d0a90194232...@hsc-cms01.ad.ufl.edu> Content-Type: text/plain; charset="us-ascii" Hello out there... We are having trouble sectioning canine bone. The samples are bone marrow cubes approximately 2cm thick and 1in wide. They were fixed for 15 hours in 10% NBF and decaled for 30 - 45 mins in RDO rapid decalcifier. They were faced in and then surface decaled again for about 30 mins. When sectioned, much of the marrow was missing and the bone was torn and shredded. We thought that maybe the samples had not been fixed sufficiently so refixed overnight in 10% NBF again. The samples were then reprocessed as they had been originally. The processing schedule used was: 70% EtOH 30mins, 80% EtOH 30mins, 95% EtOH x 2, 30mins each, 100% EtOH x 2, 30 mins each, xylene x 2, 45 mins each, paraffin x 4, 40 mins each. Again they were faced in and decaled for another 30 mins or so. This time as soon as the sections are placed on the water bath (38 degrees) they explode and/or come apart so severely sections can almost not be picked up. If sections are even obtainable they are of hor! rible quality. Does anyone have any suggestions? Thank you so much in advance! Lynda ------------------------------ Message: 2 Date: Tue, 29 Jun 2010 17:53:26 +0100 From: "Malika Benatti" <ben...@gosh.nhs.uk> Subject: [Histonet] RE: Coverslips To: <histonet@lists.utsouthwestern.edu> Message-ID: <4c2a3319.4626.003...@gosh.nhs.uk> Content-Type: text/plain; charset=UTF-8 ** Proprietary ** ** Reply Requested When Convenient ** Hi there, We use the CellPath CoverSlips 22x50mm No 1.5 they are great for hand mounting but when used with the Leica CV5030 they are a real pain. most of the CoversSlips get rejected. If anyone use the same equipment, let me know how you are getting one . Cheers, Malika ------------------------------ Message: 7 Date: Mon, 28 Jun 2010 15:06:08 -0400 From: "Weems, Joyce" <jwe...@sjha.org> Subject: [Histonet] RE: Coverslips To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <92ad9b20a6c38c4587a9febe3a30e164015dfd5...@chexcms10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" I forgot to say that the packaging has changed to their Histo Hippo theme, but they assure me they are the same product. j -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, June 28, 2010 14:29 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslips For years we have used coverslips from Mercedes - the ones in a plain white box labeled in German. This year we have begun to have problems with them being dirty and having bits of glass on them that cause air bubbles when used on the automatic coverslipper. Is anyone else having this problem? They have worked with us to try to resolve it, and have replaced several boxes, but the replacements are doing the same thing. Thanks for your input, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 8 Date: Mon, 28 Jun 2010 12:21:33 -0700 From: "Kathleen Boozer" <booze...@ah.org> Subject: Re: [Histonet] Coverslips To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, "Joyce Weems" <jwe...@sjha.org> Message-ID: <4c2893cc.4aa8.00c...@ah.org> Content-Type: text/plain; charset=US-ASCII I am having the same issues and we purchased the expensive "Platinum" to avoid that problem. They just sent me replacements and I haven't tested them all yet. Kathy Boozer, HT (ASCP), IHCQ Adventist Medical Center 10123 SE Market St. Portland, OR 97216 booze...@ah.org Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829 7875 ben...@gosh.nhs.uk >>> "Weems, Joyce" <jwe...@sjha.org> 6/28/2010 11:28 AM >>> For years we have used coverslips from Mercedes - the ones in a plain white box labeled in German. This year we have begun to have problems with them being dirty and having bits of glass on them that cause air bubbles when used on the automatic coverslipper. Is anyone else having this problem? They have worked with us to try to resolve it, and have replaced several boxes, but the replacements are doing the same thing. Thanks for your input, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 28 Jun 2010 15:36:22 -0400 From: "Sherwood, Margaret " <msherw...@partners.org> Subject: RE: [Histonet] RE: Coverslips To: "Weems, Joyce" <jwe...@sjha.org>, <histonet@lists.utsouthwestern.edu> Message-ID: <073ae2bea1c2ba4a8837ab6c4b943d9703e24...@phsxmb30.partners.org> Content-Type: text/plain; charset="us-ascii" We have been using them (24x50) and have had no problems with broken glass pieces. I do agree that they seem "dirty". Never contacted them about it. Peggy -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, June 28, 2010 3:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Coverslips I forgot to say that the packaging has changed to their Histo Hippo theme, but they assure me they are the same product. j -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, June 28, 2010 14:29 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslips For years we have used coverslips from Mercedes - the ones in a plain white box labeled in German. This year we have begun to have problems with them being dirty and having bits of glass on them that cause air bubbles when used on the automatic coverslipper. Is anyone else having this problem? They have worked with us to try to resolve it, and have replaced several boxes, but the replacements are doing the same thing. Thanks for your input, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 10 Date: Mon, 28 Jun 2010 12:41:44 -0700 From: sgoe...@xbiotech.com Subject: RE: [Histonet] RE: Coverslips To: "Weems,Joyce" <jwe...@sjha.org> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <20100628124144.9e2d9aa830e8449a2412eb1e4f2f067e.fdd3de83db....@email04.secureserver.net> Content-Type: text/plain; charset="utf-8" I just got a box of these as a "trial". They all have been far (used 1/2 the box so far)? Maybe there is gunk in your co verslipper that is causing this or possibly some glass particles? I c Sarah Goebel, B.A., HT (ASCP) < Histotechnicia XBiotech USA Inc. < Austin, Texas 78744 < (512) -------- Original Message -------- Subject: [Histonet] RE: Coverslips From: "Weems, Joyce" <[1]jwe...@sjha.o Date: Mon, June 28, 2010 12:06 pm To: "[2]histo...@lists. <[3]histo...@lists.u I forgot to say that the packaging has changed to their Histo Hippo theme, -----Original Message----- From: [4]histon [[5]mailto:histonet-boun...@lists.utsouthwestern.edu Weems, Joyce Sent: Monday, June 28, 2010 14:29 To: [6]histo...@lists.u Subject: [Histonet] Coverslips For years we have used coverslips from Mercedes - the ones in a plain white with them air bubbles when us having this problem? They and have replaced several boxes, same thing. Thanks for your input, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health E recipient(s). It may contain information that is privileged and confidential. Any unauth If you are n and reply to the sen _______________________________________________ Histonet mailing list [7]histo...@lists.utsou [8]http: Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list [9]histo...@lists.utsou [10]http: References 1. 3D"mailto://jwe...@sjha.org"/ 2. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 3. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 4. 3D"mailto://histonet-boun...@lists.utsouthwestern.edu"/ 5. 3D"mailto:histonet-bounces 6. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 7. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 8. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 9. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 10. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" ------------------------------ Message: 11 Date: Mon, 28 Jun 2010 14:48:44 -0500 From: "Mahoney,Janice A" <janice.maho...@alegent.org> Subject: RE: [Histonet] Tissue Processors To: "'Feher, Stephen'" <sfe...@cmc-nh.org>, mohamed abd el razik <k8...@yahoo.com>, "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Message-ID: <8f0ee4144e8e2f4ca1f6b051a2e5bfeea7c4a...@exchmbc2.ad.ah.local> Content-Type: text/plain; charset="iso-8859-1" I second Steve's comments about the Peloris. Jan Mahoney Omaha -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Monday, June 28, 2010 1:56 PM To: mohamed abd el razik; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tissue Processors We have 2 Peloris processors and run protocols ranging from 2 hrs to overnight. Several 2 and 4 hour protocols daily keeps a constant flow of specimens moving through the lab for those interested in LEAN small batch processing. As far as programmability, it 's all touch screen driven and easy. It's a smart processor so we don't change all solutions weekly. We only change the ones that are needed. The parameters that determine how long to use a particular solution are also programmable. Changing solutions is done by pumping out of the individual bottles and into waste containers and refilling is pumped from clean containers of solution back into the original containers. No heavy lifting required. Ours are in a separate room from our microtomy area so we hooked a simple door bell up to the local alarm jack on the back. When processing is done, we get the appropriate tone. Remote alarm is also tied in to the hospital switchboard in case a processor goes down at night or during the weekend. We really like the versatility and dependability of these processors. Steve -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Saturday, June 26, 2010 3:41 PM To: Histonet@lists.utsouthwestern.edu Subject: Fw: [Histonet] Tissue Processors i need it too as we are going to bring new tissue processor to our small lab --- On Sat, 6/26/10, Shirley Pan <sj_...@yahoo.com> wrote: From: Shirley Pan <sj_...@yahoo.com> Subject: [Histonet] Tissue Processors To: histonet@lists.utsouthwestern.edu Date: Saturday, June 26, 2010, 7:55 AM We are in the process of trying out tissue processors. Are there any users of the Leica Peloris or Thermo EG who can help us out with some opinions? Reliability, ease of changing solutions, programmability? Thanks for any help. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ------------------------------ Message: 12 Date: Mon, 28 Jun 2010 16:04:27 -0400 From: "Weems, Joyce" <jwe...@sjha.org> Subject: RE: [Histonet] RE: Coverslips To: "sgoe...@xbiotech.com" <sgoe...@xbiotech.com> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <92ad9b20a6c38c4587a9febe3a30e164015dfd5...@chexcms10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" We can coverslip by hand, but soon after the slides are coverslipped on the machine, air bubbles begin to form. We we coverslip them by hand, there is visible glass that we can feel and move if we need too. They've been good for so long, I want to make sure it's not just us. Thanks for the feedback! j ________________________________ From: sgoe...@xbiotech.com [mailto:sgoe...@xbiotech.com] Sent: Monday, June 28, 2010 15:42 To: Weems, Joyce Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Coverslips I just got a box of these as a "trial". They all have been fine so far (used 1/2 the box so far)? Maybe there is gunk in your coverslipper that is causing this or possibly some glass particles? I coverslip by hand. Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: [Histonet] RE: Coverslips From: "Weems, Joyce" <jwe...@sjha.org<mailto://jwe...@sjha.org>> Date: Mon, June 28, 2010 12:06 pm To: "histonet@lists.utsouthwestern.edu<mailto://histonet@lists.utsouthwestern.edu>" <histonet@lists.utsouthwestern.edu<mailto://histonet@lists.utsouthwestern.edu>> I forgot to say that the packaging has changed to their Histo Hippo theme, but they assure me they are the same product. j -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu<mailto://histonet-boun...@lists.utsouthwestern.edu> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Monday, June 28, 2010 14:29 To: histonet@lists.utsouthwestern.edu<mailto://histonet@lists.utsouthwestern.edu> Subject: [Histonet] Coverslips For years we have used coverslips from Mercedes - the ones in a plain white box labeled in German. This year we have begun to have problems with them being dirty and having bits of glass on them that cause air bubbles when used on the automatic coverslipper. Is anyone else having this problem? They have worked with us to try to resolve it, and have replaced several boxes, but the replacements are doing the same thing. Thanks for your input, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto://Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto://Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 13 Date: Mon, 28 Jun 2010 13:19:18 -0700 (PDT) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] Unstained Slides To: histonet@lists.utsouthwestern.edu, rick.garnh...@memorialhealthsystem.com Message-ID: <946724.39272...@web65713.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Depending on theáepitope you are trying to detect, the time could be limitedáfrom less than one month to 1-2 years. RenÎ~ J. --- On Mon, 6/28/10, rick.garnh...@memorialhealthsystem.com <rick.garnh...@memorialhealthsystem.com> wrote: From: rick.garnh...@memorialhealthsystem.com <rick.garnh...@memorialhealthsystem.com> Subject: [Histonet] Unstained Slides To: histonet@lists.utsouthwestern.edu Date: Monday, June 28, 2010, 11:54 AM Histoland, Howá is everyone storing/filing unstained slide. And how long are they good for to use for immunohistochemistry. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph:á 719-365-6926 Fax: 719-365-6373 rick.garnh...@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Mon, 28 Jun 2010 14:58:48 -0600 From: Jay Lundgren <jaylundg...@gmail.com> Subject: [Histonet] Inexpensive fume hood? To: histonet <histonet@lists.utsouthwestern.edu> Message-ID: <aanlktilumvunmyvabav6jngjgwa9kuwfnzwfw7rnn...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Can anyone recommend an inexpensive fume hood/ workstation? Not the full on metal type that hangs from the ceiling, but the Lucite bench top unit with a carbon filter. I want to avoid the capital equipment rigmarole, so it has to cost less than $1000. It is to put a small automated coverslipper under, 16" H x 20" W x 10" D . Thanks, Jay A. Lundgren, M.S., HTL (ASCP) ------------------------------ Message: 15 Date: Mon, 28 Jun 2010 14:04:42 -0700 From: Pat Laurie <foreig...@gmail.com> Subject: Re: [Histonet] Tissue Processors To: "Mahoney,Janice A" <janice.maho...@alegent.org> Cc: "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Message-ID: <aanlktimxfg5zsmucwpm8nrvzwyx2__lwesddgz_h-...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 I 3rd the previous statements about the Peloris. I had 2 pelori at my previous job, and now we currently have 3 pelori. We are also looking at getting a 4th. We use them regularly and due to the ability to use shorter programs, we run about 15 runs a day between them while still having significant downtime. I must warn you though, it is necessary to keep the service contracts on them. They are about 99% computer and have a significant number of moving parts, but they do work wonderfully. On Mon, Jun 28, 2010 at 12:48 PM, Mahoney,Janice A < janice.maho...@alegent.org> wrote: > I second Steve's comments about the Peloris. > Jan Mahoney > Omaha > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen > Sent: Monday, June 28, 2010 1:56 PM > To: mohamed abd el razik; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Tissue Processors > > We have 2 Peloris processors and run protocols ranging from 2 hrs to > overnight. Several 2 and 4 hour protocols daily keeps a constant flow of > specimens moving through the lab for those interested in LEAN small batch > processing. As far as programmability, it 's all touch screen driven and > easy. It's a smart processor so we don't change all solutions weekly. We > only change the ones that are needed. The parameters that determine how > long to use a particular solution are also programmable. Changing solutions > is done by pumping out of the individual bottles and into waste containers > and refilling is pumped from clean containers of solution back into the > original containers. No heavy lifting required. > > Ours are in a separate room from our microtomy area so we hooked a simple > door bell up to the local alarm jack on the back. When processing is done, > we get the appropriate tone. Remote alarm is also tied in to the hospital > switchboard in case a processor goes down at night or during the weekend. > > We really like the versatility and dependability of these processors. > > > Steve > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mohamed abd el > razik > Sent: Saturday, June 26, 2010 3:41 PM > To: Histonet@lists.utsouthwestern.edu > Subject: Fw: [Histonet] Tissue Processors > > > i need it too as we are going to bring new tissue processor to our small > lab > --- On Sat, 6/26/10, Shirley Pan <sj_...@yahoo.com> wrote: > > > From: Shirley Pan <sj_...@yahoo.com> > Subject: [Histonet] Tissue Processors > To: histonet@lists.utsouthwestern.edu > Date: Saturday, June 26, 2010, 7:55 AM > > > We are in the process of trying out tissue processors. Are there any users > of the Leica Peloris or Thermo EG who can help us out with some opinions? > Reliability, ease of changing solutions, programmability? Thanks for any > help. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is > faithful to the healing ministry of Jesus Christ, providing high quality > care for the body, mind and spirit of every person. > > The information contained in this communication, including attachments, is > confidential and private and intended only for the use of the addressees. > Unauthorized use, disclosure, distribution or copying is strictly > prohibited and may be unlawful. If you received this communication in > error, please inform us of the erroneous delivery by return e-mail message > from your computer. Additionally, although all attachments have been > scanned at the source for viruses, the recipient should check any > attachments for the presence of viruses before opening. Alegent Health > accepts no liability for any damage caused by any virus transmitted by this > e-mail. Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plau...@cellnetix.com ------------------------------ Message: 16 Date: Mon, 28 Jun 2010 17:57:37 -0500 From: "Amador, Amanda" <aama...@ameripath.com> Subject: [Histonet] Modified Genta To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Message-ID: <1d9b439fc446244095353dca4fc23dd20454e1d...@mwnmail00.ameripath.local> Content-Type: text/plain; charset="us-ascii" Is there someone that could help out with the Modified Genta? We tried it in the microwave and we are not sure if we are doing it correctly. We would appreciate any sample procedures to see if we need to change something. Thanks to anyone who can help out. aama...@ameripath.com<mailto:aama...@ameripath.com> ------------------------------ Message: 17 Date: Tue, 29 Jun 2010 09:53:10 -0400 From: Geoff McAuliffe <mcaul...@umdnj.edu> Subject: Re: [Histonet] what do you think? To: mohamed abd el razik <k8...@yahoo.com> Cc: Histonet@lists.utsouthwestern.edu Message-ID: <4c29fac6.1030...@umdnj.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Greetings Mohamed: The lamina propria brings small blood vessels and lymphatics close to the epithelium lining the large and small intestines. This allows nutrients to be passed back and forth and for the immune system to monitor any antigens that cross the epithelium. The relationship between the immune system and the contents of the GI tract is very complex. Lymph nodules in the lamina propria would make it thicker in some regions than in other regions. Whether the thickening you refer to is pathological or just the normal response to a variety of factors is difficult to say. Geoff mohamed abd el razik wrote: > dear histonetters > i have a quistion please regarding the thickness of lamina propria in small or larg intestine. > what is the significant of it?? does it mean inflamation and pathological condition or mean more size and so absorption of nutrients? i mean does the thickning is favorable or not? > > thanks > Mohamed Abd Elrazik > Histology dep. > Fac. of Vet. Med. > Cairo . Univ. -Egypt > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ********************************************** ------------------------------ Message: 18 Date: 29 Jun 2010 13:59:09 -0000 From: "abijag " <abija...@rediffmail.com> Subject: [Histonet] Your experience with microm HMS 740 auto stainer To: <histonet@lists.utsouthwestern.edu> Message-ID: <1277819686.s.1418.34634.webmail.rediff.com.drafts.1277819949.50...@webmail.rediffmail.com> Content-Type: text/plain; charset="UTF-8" Dear Histonetters, We would like to procure one tissue auto stainer for our histology lab(mainly H&E staining) In this respect, I request your experience about Microm HMS 740 automatic stainer. Please share your comments regarding the staining reproducibility,ease of handling and their claim about drip prevention technology. Based on your valuable feedback, we will make our decision. Thanks for all help Abi jagannath ------------------------------ Message: 19 Date: Tue, 29 Jun 2010 08:20:32 -0700 (PDT) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] Inexpensive fume hood? To: histonet <histonet@lists.utsouthwestern.edu>, Jay Lundgren <jaylundg...@gmail.com> Message-ID: <364541.78070...@web65712.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Check a Fisher catalog. The ones they sell are good for your purposes. RenÎ~ J. --- On Mon, 6/28/10, Jay Lundgren <jaylundg...@gmail.com> wrote: From: Jay Lundgren <jaylundg...@gmail.com> Subject: [Histonet] Inexpensive fume hood? To: "histonet" <histonet@lists.utsouthwestern.edu> Date: Monday, June 28, 2010, 4:58 PM Can anyone recommend an inexpensive fume hood/ workstation?á Not the full on metal type that hangs from the ceiling, but the Lucite bench top unit with a carbon filter.á I want to avoid the capital equipment rigmarole, so it has to cost less than $1000.á It is to put a small automated coverslipper under,á 16" H x 20" W x 10" D . á á á á á á á á á á á á á á á á á á á á á á á á á á á á á á á á áááThanks, Jay A. Lundgren, M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Tue, 29 Jun 2010 08:53:32 -0700 From: "Morken, Tim" <timothy.mor...@ucsfmedctr.org> Subject: RE: [Histonet] New CAP question ANP.22760 To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <1aaf670737f193429070841c6b2add4c021c361...@exmbmcb15.ucsfmedicalcenter.org> Content-Type: text/plain; charset=us-ascii Thomas and Daniel both make good points. While clinical labs do run measurable (quantitative) tests they are also running completely closed systems in which all reagents are from a certain vendor. All the reagents are validated by a vendor and all reagents are validated and calibrated for that system. Running qualitative tests is only different at the interpretive stage. All other stages should be standardized and validated to ensure the system works as intended every day, every test. IHC, of course, is a whole different ballgame. There probably is not a single lab out there that uses only one vendor and uses only one system to do IHC testing. Even if you use one automated staining system you may use antibodies from a different vendor, or you may do something outside the system that is very different than other labs use. There is such disparity between testing in different labs that it is difficult to compare results and even techniques between labs. That is the crux of the issue and what CAP and others are trying to address by tightening the validation screws. In the near future labs will need to have very robust validation and documentation for all antibodies in order to prove they are doing things correctly. That scenario is already here for the breast markers. It will come for the others as well. Right now the instances in which solid validation methods are absolutely necessary is when validating the breast markers ER, PR and HER2, which all are used as stand-alone tests to determine treatment, and for ASR's. If you use a vendor kit with all reagents supplied you must still prove it works in your lab as intended ("verify outside data." The "outside data" is the claim that it stains in a certain way). You must run cases of various expressions and show your lab gets the proper results. Third party verification is very helpful, that is, comparing your results to that of other labs on the same material (CAP surveys, or set up a partnership with another lab). If you use only the antibody for one of those markers, or mix and match components outside a FDA-approved kit, then you are responsible for performing a comprehensive validation of the test. Its worth noting that every news article I've seen about pathology/histology labs in the last several years has been about failures to do the breast panel tests correctly. We are under a microscope these days and it will pay off to make sure you are doing the tests well! Most other markers in IHC are ancillary tests that are run in conjunction with other tests to determine a diagnosis. Those antibodies still need to be validated in your system but don't require a large sample of cases. They have been validated as IVD's by the vendor so just need to be shown they work as intended with your lab's methods and instruments. But I still think it is worthwhile to do a validation that includes a range of expressions along with irrelevant tissue (negative). That will help calibrate your expectations about how the antibody works and give you a baseline to refer to if problems arise. Running one or two slides to make sure it "works" is not really satisfactory. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Saturday, June 26, 2010 1:45 PM To: Daniel Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 Daniel, I think your observations are very good. Which brings me again to a point I was trying to make earlier. Anatomic Pathology is not the same as the General Clinical Lab or it's close counterparts. It almost seems like this is overblown re: validation. As you've pointed out, the interpretation is up to the pathologist. Patients (prostate, i.e.) will correlate with other tests. When known positives demonstrate properly you're valid. Even automated systems can use algorithms to score strength of positivity...which varies yet will show as positive (valid). You receive a new batch of Ab you run it on a known positive, that's the bottom line. It seems somehow CAP or clinical lab (trained) folks want more than that. And again (as you pointed out) the appreciation for the differences in how we operate as laboratorians seems to fall by the wayside. As you mention pushing 20, 50, 100 is not feasible (and frankly unnecessary). I don't know where the answer lies in making everyone happy here. I do know that some thinking outside the box and trying understand AP is required. We all want good patient care, but one size does not fit all. Thanks, tj -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Daniel Sent: Saturday, June 26, 2010 11:08 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New CAP question ANP.22760 One question I think a lot of you are not considering is that the clinical laboratory usually tests for analytes present in most patients. This allows the clinical lab to more easily run validations with hundreds of patients, using statistical tools to analyze the precision, specificity and sensitivity of the test. What most of those concerned with this new CAP standard (and I count among them) is that our testing often targets a very rare population of patients. As such, an extensive validation is much more difficult to establish. It then makes the statistical models used in clinical tests much less valuble since the accuracy of these formulas decrease with smaller sample sizes. Additionally, the pathology of the clinical tests are reported as a measurement which does not have an intrinsic value. It must be interpreted by a clinician to give value to the patient. For example, the Blood Urea Nitrogen test value range usually is between 7-20 mg/dL while values above 50 are considered a marker of poor kidney function. Elevated values, however, do not mean that kidney functions are impaired but instead may be due to other pathologies such as congestive heart failure, gi bleeding, certain steroids and even diet. For histopathology even relatively common antibodies such as pan-T cell marker CD3 usually only stain 75% of T cell neoplasms. A negative result for that minority does not indicate that the tumor does not have a T cell lineage. It relies instead on the pathologist interpreting the result much as the clinician looks at the BUN value in relation to other factors in the patient's presentation. When we validate this kind of antibody, do we perform testing then on T cells and B cells (give me a good tonsil), on malignant samples or some combination of the two? What is a reasonable number of tests to run then? If I choose 10 normal lymphatic tissue blocks for my routine T/B cell marking (it can't be as extensive as ER/PR can it?) how many samples should I choose for my malignant population? If I use another 10 anyone with a background in statistics will tell you that the sample size is too small to interpret accuracy. If I push it up to 20, 50 or 100 I would be certain that many laboratories would not be able to afford the cost of the validation. This would then push many good laboratories out of the business of IHC with the unintended result of delaying diagnoses and increasing patient costs by driving testing to fewer and fewer testing outlets. CAP's new path with ER/PR seems to be trying to achieve a noble end of improving quality in the laboratory but without an understanding of the complexities and consequences of the method they are implementing. Dan -----Original Message----- >From: Jesus Ellin <jel...@yumaregional.org> >Sent: Jun 26, 2010 6:28 AM >To: "Morken, Tim" <timothy.mor...@ucsfmedctr.org>, >histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] New CAP question ANP.22760 > >I have been reading the post to this question and it seems to me that there are different standards depending on the lab that is operating the methodology. I do agree that the core lab for years have had the instruction and training in the performance of validation. One thing that comes to mind as well is why has histology not had this training? Why are we not getting this from our certification agency, our professional societies and biggest reason where is our standardization. It seems to me that with all these regualtions in plac for so long, why were we missed. Is it because when inspected through CAP we are being inspected by a pathologist rather than a histo tech? These are some of the questions at hand. I to see new standards within the CAP checklist as well as other regulatory organizations that will affect the future of the Anatomic Pathology community. But I think we need is to provide a underlying architecture for our peers, so that we can begin the transition to the future. This is only the beginning, there is still Digital Image Analysis and Telepathology. It funny we are looking to become a hybrid of radiology and the core lab, but with the best of both worlds. Tim great structure for the validation study. > > >-----Original Message----- >From: histonet-boun...@lists.utsouthwestern.edu on behalf of Morken, >Tim >Sent: Wed 6/23/2010 9:48 AM >To: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] New CAP question ANP.22760 > >Joe, > >You wrote : The folks in the 'clinical' lab have been performing more comprehensive and complex validation procedures for a very long time ..." > >Those were my thoughts exactly. While the person replying may or may not have specific histology experience she will have clinical lab experience (however, my guess is that she is exposed to histology regularly at CAP). Clinical labs have a bit of an easier time, actually, because they validate primarily to known concentration controls - analytical controls manufactured at a range of known concentrations for instance. The institution then adds in their normal controls for validation. > >As far as the current question about validating a new lot of reagent the best practice is to run parallel tests on the same machine. If that is not easily possible on a particular manufacturer's instrument then the question should be asked of them: Why not? If this is a requirement the manufacturer should provide an easy path to meeting the requirement. However, if that is not the case then the institution simply writes a procedure to get around the inadequacies of the instrument (Maybe the vendor can help with that). Then follow the procedure. That should satisfy inspectors. > >An "...appropriate panel of tissues..." is whatever the institution deems appropriate for the given antibody or reagent. This is a perfect place for tissue arrays. You can make your own or buy them. > >IHC must meet CLIA validation guidelines but since IHC is generally qualitative the requirements must be understood and methods adapted to a qualitative scenario. Several IHC and Histotechnology books discuss the subject at length (Taylor, Dabbs, Bancroft for instance). > >Below is a brief overview of how to do that. (for more in-depth info this was covered in an NSH teleconference I gave last year - PowerPoint, audio and references available from NSH-, and will be covered in a similar workshop at NSH in Seattle this year). > > >1) >CAP General Validation >CAP GEN.42020-42163 Test Method Validation Follows CLIA CFR Sec >493.1253 Does not apply well to IHC (IHC is usually qualitative) > >But the general principle applies: >The laboratory must have data on each test's accuracy, precision, analytic sensitivity, interferences and reportable range. > >Unmodified FDA-cleared or approved tests: the lab may use manufacturer information or published reports but lab must verify outside data. > >Non-FDA cleared: Lab MUST verify or establish analytic accuracy, precision, sensitivity, specificity and reportable range. > >2) Validation includes: >Accuracy: > Compare results with New antibody to a previously validated antibody on the same tissues > >Precision: > Test samples with varying antigen expression > Intra-run, Inter-run tests, 10 slides each (reproducibility) > >Sensitivity: > True Positive vs False Negative (higher % FN = less sensitive) > >Interferences [Specificity]: > True Negative vs False Positive (Higher % FP = less specific) > Delineate what could interfere to give a false positive or false negative result. > >Reportable Range > Establish a scoring system > Provide the definition of a positive result > >3)Sensitivity > >Analytic Sensitivity: > Lowest amount of substance detectable by the test > Can only be done with controls of known concentration > >Diagnostic Sensitivity: > Ability of the test to determine true diagnostic positive verses false negative (higher % FN = less sensitive) > Requires comparison to a previously validated antibody > >IHC Sensitivity: > Extent to which an antibody can be diluted and still achieve target recognition. NOTE: This is determined by antibody AND detection system! > > > >4) Specificity: > >Analytic Specificity > Accuracy on tests of known positive and negative controls > Controls of known concentration > Determine what could "Interfere" to confound the result > > >Diagnostic Specificity > Ability of a test to determine true diagnostic negative verses false positives (Higher % FP = less specific) > Requires comparison to a previously validated antibody > > >IHC Specificity > Ability of an antibody to bind exclusively to its particular antigen in the absence of staining of other molecules > Or, staining of other structures in addition to target >structures/cells > >(Sensitivity and Specificity adapted from: Theoretical and Practical >Aspects of Test Performance, in Immunomicroscopy, Taylor & Cote, 2005) > >Tim Morken >Supervisor, Histology / IPOX >UCSF Medical Center >San Francisco, CA > > >-----Original Message----- >From: histonet-boun...@lists.utsouthwestern.edu >[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of >jmye...@aol.com >Sent: Tuesday, June 22, 2010 6:51 PM >To: tjas...@copc.net >Cc: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] New CAP question ANP.22760 > >Tom: > >As much as I agree with your acknowledgment that its seems a bit odd >for the CAP to have a blood-banker responding to AP-related issue, I'm >actually not surprised. The folks in the 'clinical' lab have been >performing more comprehensive and complex validation procedures for a >very long time, and they wonder why IHC isn't expected to follow the >same requirements as chemistry, immunology, etc. -- IHC is, after all, >an awful lot like ELISA. And rightfully so, because IHC is, under CLIA >(which supersedes CAP), considered highly-complex, non-waived testing >-- and is, therefore, subject to the same Quality Systems regulations >(in particular, 42CFR493.1252-1256, 1273, and 1281) as the testing performed in other areas of the lab. > >Could it be that, because AP produces qualitative results that are >interpreted by a pathologist and CP produces quantitative results that >are interpreted by an analyzer, we somehow think that CLIA rules don't >apply to IHC? I certainly don't have the answer to that, but it make >me wonder what the future holds. As witnessed by some of the newest >CAP 'standards' (including the question in question...no pun intended), >e.g. ER/PR, where a minimum of 20 positive and 20 negative specimens >must be tested, and where 10 of the positives must be weakly positive >-- an acknowledgment that validation specimens must be carefully >selected in order to obtain appropriate results), it certainly doesn't >appear that the regulation of IHC testing is going to become more relaxed. > >Joe Myers, M.S., CT(ASCP) > >------------------------------ > >Message: 12 >Date: Fri, 18 Jun 2010 12:38:07 -0700 >From: "Thomas Jasper" <tjas...@copc.net> >Subject: RE: [Histonet] New CAP question ANP.22760 >To: "Mark Tarango" <marktara...@gmail.com> >Cc: _histo...@lists.utsouthwestern.edu_ >(mailto:histonet@lists.utsouthwestern.edu) > >Mark, > >Did you notice the credentials from this CAP representative? MT with a >Blood Bank specialty I believe. What I glean from that is...more than >likely this person does not grasp the logistics of "contemporaneously" >staining identical Abs from separate lots. She also likely does not >understand the logistical application for detection and automation >either. > >I'm not trying to be overly critical of this person. I'm sure she is >quite intelligent and would not have the MT/SBB if she wasn't >intelligent. It comes down to a lack of understanding Anatomic >Pathology testing application re: automated IHC. I believe this is a >common problem in and out of CAP. Many lab directors and other folks in >positions of authority without AP/Histology/Cytology backgrounds seem >to believe that broad clinical lab modalities apply to Anatomic Path >scenarios. I used to refer to this in my former position as - "Trying >to put the yoke of clinical lab onto anatomic path." We are >laboratorians, but in many instances do not fit the general clinical >lab mold. > >It's unfortunate that CAP has put this person in the position to >respond. It is apparent to me that she's not grasping the particulars >here. She probably never will unless she decides to go into a working, >automated IHC "tissue" lab and take the time to ask questions and >understand (learn) what we're all about. > >Thanks, >Tom Jasper > >Thomas Jasper HT (ASCP) BAS >Histology Supervisor >Central Oregon Regional Pathology Services Bend, OR 97701 >_______________________________________________ ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 79, Issue 37 **************************************** ********************************************************************************************************* This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. 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