Vanessa, I work with a lot of great histology folks and what I know about it is that eosin bleeding and blue-haze problems usually occur as a consequence of either not fully removing all of the water during the final absolute alcohol rinse step, and/or not fully removing all of the alcohol during the clearing step. If the water and alcohol are completely removed, bleeding and haze will not occur. I hope this helps.
Beth Austin-Sell CBG Biotech -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 80, Issue 4 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: control tissue for Hall's bile stain (Robert Richmond) 2. RE: BILLING QUESTION (Cynthia Pyse) 3. RE: ER and PR validation (jmye...@aol.com) 4. Thomas Crowell is out of the office. (thomas.crow...@novartis.com) 5. Looking for MIN6 cell line (Debra Baluch) 6. bat wing histology (Amos Brooks) 7. RE: BILLING QUESTION (Feher, Stephen) 8. xylene substitute (Vanessa Avalos) ---------------------------------------------------------------------- Message: 1 Date: Fri, 2 Jul 2010 13:48:52 -0400 From: Robert Richmond <rsrichm...@gmail.com> Subject: [Histonet] Re: control tissue for Hall's bile stain To: histonet@lists.utsouthwestern.edu Message-ID: <aanlktildu0qipr9rjajgqqonoaanf2yk1skc0houk...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hall's bile stain - not really a stain but a reaction, like the Perls prussian blue reaction for iron - uses trichloracetic acid and ferric chloride to oxidize yellow-brown bilirubin to bright green biliverdin. Or so I've been told - I've never seen one. Something I've wondered about for a long time: could Hall's reaction be used to demonstrate meconium in macrophages in the chorion of the human placenta? These can be hard to see, particularly if you don't have a very good microscope. - If this would work, it would provide a ready source for controls, at least for the hospital pathologist. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 2 Date: Fri, 2 Jul 2010 13:51:51 -0400 From: "Cynthia Pyse" <cp...@x-celllab.com> Subject: RE: [Histonet] BILLING QUESTION To: "'Sara Baldwin/mhhcc.org'" <sbald...@mhhcc.org>, <histonet@lists.utsouthwestern.edu> Message-ID: <000001cb1a0f$40ca1c10$c25e54...@com> Content-Type: text/plain; charset="us-ascii" We have a contract with the hospitals, this comes from our billing manger. We bill the hospitals, then they bill the patient. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Friday, July 02, 2010 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BILLING QUESTION Histonetters: If you do some work for another hospital (Histology) can you bill the hospital or do you have to bill the patient directly? Is there a statute or Regulation out there about this? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Fri, 2 Jul 2010 13:54:26 EDT From: jmye...@aol.com Subject: RE: [Histonet] ER and PR validation To: histonet@lists.utsouthwestern.edu Cc: dorothy.l.w...@healthpartners.com Message-ID: <8f84.3cbd32f0.395f8...@aol.com> Content-Type: text/plain; charset="US-ASCII" This paper can also be downloaded, indirectly, from the CAP's website: _http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165-134.7.e48_ (http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165-134.7.e48) Happy (re-)validating, Joe ------------------------------ Message: 12 Date: Fri, 2 Jul 2010 10:23:08 -0500 From: "Webb, Dorothy L" <dorothy.l.w...@healthpartners.com> Subject: [Histonet] ER and PR validation To: _'histonet@lists.utsouthwestern.edu'_ (mailto:'histonet@lists.utsouthwestern.edu') In a recent article written by 4 pathologists, including Dr. Elizabeth Hammond and Dr. Patrick Fitzgibbons, the recommendations are now including testing with a lab that has validated it's assay against clinical outcomes. How is everyone planning on fulfilling this requirement inasmuch as we are not aware of any lab in our metro area with this kind of compliance. Thanks, as always, for any guidance in this area! If you would like a reprint of this article, email is pf...@stjoe.org and the article is entitle "Recommendations for Validating Estrogen and Progesterone Receptor immunohistochemistry Assays". Dorothy Webb, HT Regions Histology Technical Supervisor ------------------------------ Message: 4 Date: Fri, 2 Jul 2010 15:32:28 -0400 From: thomas.crow...@novartis.com Subject: [Histonet] Thomas Crowell is out of the office. To: histonet@lists.utsouthwestern.edu Message-ID: <of9a757d25.2860015a-on85257754.006b57cd-85257754.006b5...@ah.novartis.com> Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 07/02/2010 and will not return until 07/13/2010. Please contact Kelly Miner at 617-871-5122 if you have any questions regarding clinical trial samples. ------------------------------ Message: 5 Date: Fri, 2 Jul 2010 13:14:00 -0700 From: Debra Baluch <page.bal...@asu.edu> Subject: [Histonet] Looking for MIN6 cell line To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <80bbc916bec29a498784913d007dd4a24dbdc0a...@ex10.asurite.ad.asu.edu> Content-Type: text/plain; charset="us-ascii" Does anyone know where I can obtain the MIN6 beta cell line derived from mouse pancreas? They are listed and used in many papers but do not list the source. I have tried ATCC and suggested cell banks in ECACC (England), DSMZ (Germany), and JCRB (Japan) but they are not listed. Thanks, Page Arizona State University/SoLS -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jmye...@aol.com Sent: Friday, July 02, 2010 10:54 AM To: histonet@lists.utsouthwestern.edu Cc: dorothy.l.w...@healthpartners.com Subject: RE: [Histonet] ER and PR validation This paper can also be downloaded, indirectly, from the CAP's website: _http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165-134.7.e48_ (http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165-134.7.e48) Happy (re-)validating, Joe ------------------------------ Message: 12 Date: Fri, 2 Jul 2010 10:23:08 -0500 From: "Webb, Dorothy L" <dorothy.l.w...@healthpartners.com> Subject: [Histonet] ER and PR validation To: _'histonet@lists.utsouthwestern.edu'_ (mailto:'histonet@lists.utsouthwestern.edu') In a recent article written by 4 pathologists, including Dr. Elizabeth Hammond and Dr. Patrick Fitzgibbons, the recommendations are now including testing with a lab that has validated it's assay against clinical outcomes. How is everyone planning on fulfilling this requirement inasmuch as we are not aware of any lab in our metro area with this kind of compliance. Thanks, as always, for any guidance in this area! If you would like a reprint of this article, email is pf...@stjoe.org and the article is entitle "Recommendations for Validating Estrogen and Progesterone Receptor immunohistochemistry Assays". Dorothy Webb, HT Regions Histology Technical Supervisor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 2 Jul 2010 16:14:36 -0400 From: Amos Brooks <amosbro...@gmail.com> Subject: [Histonet] bat wing histology To: mchad...@gmail.com, histonet@lists.utsouthwestern.edu Message-ID: <aanlktikzxry16avvvfhaohpsghmys-ida0mivtemc...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi, I do hope you are looking at cross sections of the wing and not the flat. That would be very difficult indeed. For good cross sections I would try a "Swiss Roll". This is a way of demonstrating a large amount of cross sectional area in small space. Take the membrane and fix it by submersion in the fixative of your choice. Prior to processing roll the whole membrane up then cut the membrane log into sections small enough to fit in a cassette. You can use foam biopsy pads to support this shape. Embed it and section it on edge to show a long coiled membrane. The hairs should be able to be displayed in this way as well. To show a lot of membrane at the same time you could place multiple rolls in one cassette. This should work well. Good Luck, Amos Message: 21 Date: Thu, 1 Jul 2010 11:46:17 -0400 From: Mohit Chadha <mchad...@gmail.com> Subject: [Histonet] bat wing histology To: histonet@lists.utsouthwestern.edu Message-ID: <aanlktikkgxt1_uy5qkdvhp04ftyiuocwy9xnvbleq...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hello everyone, This is my very first post and I am desperately looking for help. I am new to histology, so any help would be much appreciated. I am studying the peripheral sensory innervation of bat wings. As a first step, I would like to demonstrate the innervation pattern on the different parts of the wing membrane (a whole mount of the wing?). Second, I would like to demonstrate the mechanoreceptor make-up of the tiny hairs on the wing membrane. Bat wings are highly elastic, with numerous folds, a thickness of about 35-45 microns (in the species I study), a network of thin collagen bundles, and pigmented superficial epidermal layers. I could provide more information if required. Hoping to hear back from the members. Thank you. ------------------------------ Message: 7 Date: Fri, 2 Jul 2010 17:18:14 -0400 From: "Feher, Stephen" <sfe...@cmc-nh.org> Subject: RE: [Histonet] BILLING QUESTION To: "Cynthia Pyse" <cp...@x-celllab.com>, "Sara Baldwin/mhhcc.org" <sbald...@mhhcc.org>, <histonet@lists.utsouthwestern.edu> Message-ID: <73a7ed895ee0c24d9267ed814911df1912b75...@exchange.cmc-nh.org> Content-Type: text/plain; charset="us-ascii" You can contract for services to another lab or hospital and depending upon the terms of the contract, you may be able to bill the patient. For example, you send all of your HER2's and ER/PR to another lab to be stained and quantified into a score. You may contract with the other lab for them to charge you a flat fee for the service and you will bill the patient for 88361x3. The other side is that you can contract for the other hospital to do the test, bill the patient and your hospital pays no fee but misses out on some of the compensation. Steve -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Friday, July 02, 2010 1:52 PM To: 'Sara Baldwin/mhhcc.org'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BILLING QUESTION We have a contract with the hospitals, this comes from our billing manger. We bill the hospitals, then they bill the patient. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Friday, July 02, 2010 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BILLING QUESTION Histonetters: If you do some work for another hospital (Histology) can you bill the hospital or do you have to bill the patient directly? Is there a statute or Regulation out there about this? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Fri, 2 Jul 2010 15:20:52 -0700 From: "Vanessa Avalos" <vava...@allergydermatology.com> Subject: [Histonet] xylene substitute To: <histonet@lists.utsouthwestern.edu> Message-ID: <000301cb1a34$dbdd5c10$939814...@com> Content-Type: text/plain; charset="us-ascii" I am taking a chance that this is still a working email!! I came across an old post on histonet regarding Xylene subs with a linear stainer. I was wondering if you made the switch, and if so could you share the brand and your H&E protocol. I have had some issues with Eosin bleeding, slides very eosinophilic and blue haze on the slide. Not all at once but its enough to drive me crazy!! Your help would be appreciated. V.Avalos ADS, INC Fax:602-277-2134 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 80, Issue 4 *************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet