Hi All,
Has anyone ever used a Biotin block in their primary anitbody diluent? I have
been having problems with nonspecific staining, which I suspect is due to
endogenous biotin. I plan on decreasing my antigen retrieval time, as someone
has told me that an antigen retrieval that is too vigorous may cause the
unmasking of biotin, and its subsequent staining. I would like to know if
anyone has had any luck using a biotin block in their diluent because I may try
that as well.
Thanks,
Jennifer Campbell
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