See these articles- keep formalin fixation to <12 hrs. Vinod Shidham, Ganesh Shidham (2000) Staining Method to Demonstrate Urate Crystals in Formalin-Fixed, Paraffin-Embedded Tissue Sections. Archives of Pathology & Laboratory Medicine: Vol. 124, No. 5, pp. 774-776.
Shidham V, Chivukula M, Basir Z, Shidham G. Mod Pathol. 2001 Aug;14(8):806-10. Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 [email protected] -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of HOOD MS. GLENDA Sent: Wednesday, July 07, 2010 12:39 PM To: [email protected] Subject: [Histonet] RE: gout crystals It is true that gout crystals are water-soluble, and we are all taught that... but a few years ago there was a published article (JOH??) that showed that many crystals DO survive after water-based fixation. Since you already have the specimen in formalin, at least try processing and see if they can be demonstrated. Couldn't hurt, not nearly as much as the patient would for a re-biopsy! Glenda F. Hood, M.Ed., HT(ASCP) Instructor and Program Director Histotechnology Program Tarleton State University 817-926-1101 x6 -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of [email protected] Sent: Wednesday, July 07, 2010 11:22 AM To: [email protected] Subject: Histonet Digest, Vol 80, Issue 7 Send Histonet mailing list submissions to [email protected] To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to [email protected] You can reach the person managing the list at [email protected] When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Rnase free slides? (Caroline Bass) 2. Gout Crytals (Behnaz Sohrab) 3. RE: Gout Crytals (Weems, Joyce) 4. Re: Gout Crytals (Jennifer MacDonald) 5. RE: Gout Crytals ([email protected]) 6. Schmitz, Sandy is out of the office. ([email protected]) 7. RE: Gout Crytals (Joyce Cline) 8. Re: bat wing histology (Mohit Chadha) 9. RE: Gout Crytals (Douglas,Joseph) 10. used histology equipment ([email protected]) 11. Re: used histology equipment (Drew Meyer) 12. Re: Rnase free slides? (Emily Sours) 13. Re: bat wing histology (Amos Brooks) 14. Has anyone used a biotin block in their antibody diluent? (Jennifer Campbell) 15. Biotin block (Jim Reilly) 16. RE: Has anyone used a biotin block in their antibody diluent? (Mauger, Joanne) 17. Re: used histology equipment ([email protected]) 18. RE: used histology equipment (Douglas,Joseph) 19. RE: used histology equipment (Douglas,Joseph) 20. Reprocess ([email protected]) 21. Re: Reprocess (Catherine Simonson) 22. RE: Reprocess (Cheri Miller) 23. RE: Reprocess (Cheri Miller) 24. RE: Reprocess (Mahoney,Janice A) ---------------------------------------------------------------------- Message: 1 Date: Tue, 06 Jul 2010 15:49:58 -0400 From: Caroline Bass <[email protected]> Subject: [Histonet] Rnase free slides? To: <[email protected]> Message-ID: <c8590126.2888%[email protected]> Content-Type: text/plain; charset="US-ASCII" Hello, I'm doing RNA work for the first time. My plan is to take a fresh rat brain, block quickly, freeze by immersing in dry-ice cooled isopentane, storing at -80, collecting tissue sections (thickness will be determined, somewhere between 20 and 300 um), and punching the particular regions I need out of the sections. I will then isolate RNA from the punches for qPCR analysis. Questions: 1) does this sound like a viable plan? 2) and suggestions, what to be careful of? 3) where do I have to be careful of Rnase, should I use disposable blades, cleaned with Rnase away? 4) where can I find Rnase free slides, or should I just make my own. I usually use charged slides. Any and all suggestions will be appreciated. I'm new to this and don't know where I will have problems. Thanks! Caroline ------------------------------ Message: 2 Date: Tue, 06 Jul 2010 13:12:49 -0700 From: "Behnaz Sohrab" <[email protected]> Subject: [Histonet] Gout Crytals To: <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset=US-ASCII Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alcohols? Tissue has been fixed in formalin.? Thank you ------------------------------ Message: 3 Date: Tue, 6 Jul 2010 16:35:41 -0400 From: "Weems, Joyce" <[email protected]> Subject: RE: [Histonet] Gout Crytals To: Behnaz Sohrab <[email protected]>, "[email protected]" <[email protected]> Message-ID: <92ad9b20a6c38c4587a9febe3a30e164015e968...@chexcms10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" Should skip the formalin!!! They are water soluable... :>( -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Behnaz Sohrab Sent: Tuesday, July 06, 2010 16:13 To: [email protected] Subject: [Histonet] Gout Crytals Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alcohols? Tissue has been fixed in formalin.? Thank you _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 4 Date: Tue, 6 Jul 2010 13:50:19 -0700 From: Jennifer MacDonald <[email protected]> Subject: Re: [Histonet] Gout Crytals To: "Behnaz Sohrab" <[email protected]> Cc: [email protected], [email protected] Message-ID: <of6246d4bb.19fa5577-on88257758.0072608e-88257758.00727...@mtsac.edu> Content-Type: text/plain; charset="US-ASCII" Uric acid crystals are water soluble. Avoid formalin and fix in absolute alcohol and start the processing of the tissue in absolute alcohol. Jennifer "Behnaz Sohrab" <[email protected]> Sent by: [email protected] 07/06/2010 01:28 PM To <[email protected]> cc Subject [Histonet] Gout Crytals Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alcohols? Tissue has been fixed in formalin.? Thank you _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Tue, 06 Jul 2010 13:55:24 -0700 From: [email protected] Subject: RE: [Histonet] Gout Crytals To: "Behnaz Sohrab" <[email protected]> Cc: [email protected] Message-ID: <20100706135524.9e2d9aa830e8449a2412eb1e4f2f067e.8ce03ce3da....@email04. secureserver.net> Content-Type: text/plain; charset="utf-8" If you have fixed in formalin the gout crystals are all gone!!!!& nbsp; Have to start over with a new sample if possible. The crystals Sarah Goebel, B.A. Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, (512)386-5107 -------- Original Message -------- Subject: [Histonet] Gout Crytals From: "Behnaz Sohrab" <[1]sohra...@ah. Date: Tue, July 06, 2010 1:12 pm To: <[2]histo...@lis Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alc Thank you _______________________________________________ Histonet mailing list [3][email protected] [4]http: References 1. 3D"mailto://[email protected]"/ 2. 3D"mailto://[email protected]"/ 3. 3D"mailto://[email protected]"/ 4. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"; ------------------------------ Message: 6 Date: Tue, 6 Jul 2010 16:00:56 -0500 From: [email protected] Subject: [Histonet] Schmitz, Sandy is out of the office. To: [email protected] Message-ID: <of180dfe40.bfc56a43-on86257758.00737178-86257758.00737...@leica-microsy stems.com> Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 07/05/2010 and will not return until 07/07/2010. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ ------------------------------ Message: 7 Date: Tue, 6 Jul 2010 16:56:52 -0400 From: Joyce Cline <[email protected]> Subject: RE: [Histonet] Gout Crytals To: "[email protected]" <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset="us-ascii" This works for us. Process normally, we usually fix in 100% alcohol. Cut slide Formula 83 or Xylene 20 seconds Form 83 or Xylene/100% alcohol mixed 50/50 for 20 seconds Eosin for 20 seconds 100% alcohol 20 seconds 100% alcohol 20 seconds Form 83 or Xylene/100% alcohol mixed 50/50 for 20 seconds Form 83 or Xylene for 20 seconds coverslip normally Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ________________________________________ From: [email protected] [[email protected]] On Behalf Of Behnaz Sohrab [[email protected]] Sent: Tuesday, July 06, 2010 4:12 PM To: [email protected] Subject: [Histonet] Gout Crytals Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alcohols? Tissue has been fixed in formalin.? Thank you _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ------------------------------ Message: 8 Date: Tue, 6 Jul 2010 17:10:17 -0400 From: Mohit Chadha <[email protected]> Subject: Re: [Histonet] bat wing histology To: Amos Brooks <[email protected]> Cc: [email protected] Message-ID: <[email protected]> Content-Type: text/plain; charset=ISO-8859-1 Thank you everyone for replying, much appreciated. Having also talked to people in my dept, I have a rudimentary protocol ready. Of course, I will have to tweak it to see what works. I will be using anti PGP9.5 antibody for neuronal immunology. I am still not sure how to section the wing. In most likelihood, I will be using freezing sliding microtome. The "swiss roll" method sound good and I will definitely try it. I am thinking that since the wing membrane is thin (~30 um), I will also try to use the whole mount of small pieces. Any other thoughts and advice would be appreciated. Thank you, Mohit Chadha, Univ of Maryland. On Fri, Jul 2, 2010 at 4:14 PM, Amos Brooks <[email protected]> wrote: > Hi, > I do hope you are looking at cross sections of the wing and not the > flat. That would be very difficult indeed. For good cross sections I would > try a "Swiss Roll". This is a way of demonstrating a large amount of cross > sectional area in small space. Take the membrane and fix it by submersion in > the fixative of your choice. Prior to processing roll the whole membrane up > then cut the membrane log into sections small enough to fit in a cassette. > You can use foam biopsy pads to support this shape. Embed it and section it > on edge to show a long coiled membrane. The hairs should be able to be > displayed in this way as well. To show a lot of membrane at the same time > you could place multiple rolls in one cassette. This should work well. > > Good Luck, > Amos > > > Message: 21 > Date: Thu, 1 Jul 2010 11:46:17 -0400 > From: Mohit Chadha <[email protected]> > Subject: [Histonet] bat wing histology > To: [email protected] > Message-ID: > <[email protected]> > Content-Type: text/plain; charset=ISO-8859-1 > > Hello everyone, > > This is my very first post and I am desperately looking for help. I am new > to histology, so any help would be much appreciated. > > I am studying the peripheral sensory innervation of bat wings. As a first > step, I would like to demonstrate the innervation pattern on the different > parts of the wing membrane (a whole mount of the wing?). Second, I would > like to demonstrate the mechanoreceptor make-up of the tiny hairs on the > wing membrane. > > Bat wings are highly elastic, with numerous folds, a thickness of about > 35-45 microns (in the species I study), a network of thin collagen bundles, > and pigmented superficial epidermal layers. > I could provide more information if required. > > Hoping to hear back from the members. > Thank you. > ------------------------------ Message: 9 Date: Tue, 6 Jul 2010 16:22:46 -0500 From: "Douglas,Joseph" <[email protected]> Subject: RE: [Histonet] Gout Crytals To: 'Jennifer MacDonald' <[email protected]>, Behnaz Sohrab <[email protected]> Cc: "[email protected]" <[email protected]>, "[email protected]" <[email protected]> Message-ID: <dacc27f195e63d44bdf7adc5c52051af1f02004...@dcpwvmbxc0vs1.mdanderson.edu > Content-Type: text/plain; charset="us-ascii" REMOVE -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Jennifer MacDonald Sent: Tuesday, July 06, 2010 3:50 PM To: Behnaz Sohrab Cc: [email protected]; [email protected] Subject: Re: [Histonet] Gout Crytals Uric acid crystals are water soluble. Avoid formalin and fix in absolute alcohol and start the processing of the tissue in absolute alcohol. Jennifer "Behnaz Sohrab" <[email protected]> Sent by: [email protected] 07/06/2010 01:28 PM To <[email protected]> cc Subject [Histonet] Gout Crytals Please refresh my memory!! Processing For Gout Crystal !1 Do I skip all alcohols? Tissue has been fixed in formalin.? Thank you _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 6 Jul 2010 18:29:09 -0400 From: <[email protected]> Subject: [Histonet] used histology equipment To: "'histonet'" <[email protected]> Message-ID: <!~!UENERkVCMDkAAQACAAAAAAAAAAAAAAAAABgAAAAAAAAAdq7zShuC4ki+F530qNQCZMKA [email protected]> Content-Type: text/plain; charset="us-ascii" Does anyone know of a reputable dealer for used equipment? ------------------------------ Message: 11 Date: Tue, 6 Jul 2010 18:55:53 -0400 From: Drew Meyer <[email protected]> Subject: Re: [Histonet] used histology equipment To: [email protected] Cc: histonet <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset=ISO-8859-1 Absolutely... Southeast Pathology Instrument Service out of Charleston, SC. The owner's name is Michael Dietrich. I've done business with him before and they are great people, very honest and they stand behind their instruments. I would highly recommend them to anyone. Contact Michael directly and tell him Drew Meyer from Atlanta referred you. http://southeastpathology.com/ Drew On Tue, Jul 6, 2010 at 18:29, <[email protected]> wrote: > Does anyone know of a reputable dealer for used equipment? > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 12 Date: Tue, 6 Jul 2010 19:24:44 -0400 From: Emily Sours <[email protected]> Subject: Re: [Histonet] Rnase free slides? To: Caroline Bass <[email protected]>, [email protected] Message-ID: <[email protected]> Content-Type: text/plain; charset=UTF-8 Honestly, I think RNases are a bunch of hooha. If you're being careful anyway because you're doing a PCR, that should be enough. Wear gloves, be sterile. When I worked with a Russian post-doc, she said she did RNA in situ hybridization without gloves and it worked. Of course, god only knows what her protocol was, as she had some crazy stories about Russian labs. Emily -- Dark Pictures, thrones and stones that pilgrims kiss And poems that take a thousand years to die But ape the immortality of this Red label on a little butterfly. -Vladimir Nabokov, concluding stanza of ???A Discovery??? 1941. On Tue, Jul 6, 2010 at 3:49 PM, Caroline Bass <[email protected]> wrote: > Hello, > > I'm doing RNA work for the first time. My plan is to take a fresh rat brain, > block quickly, freeze by immersing in dry-ice cooled isopentane, storing at > -80, collecting tissue sections (thickness will be determined, somewhere > between 20 and 300 um), and punching the particular regions I need out of > the sections. I will then isolate RNA from the punches for qPCR analysis. > > Questions: > > 1) does this sound like a viable plan? > 2) and suggestions, what to be careful of? > 3) where do I have to be careful of Rnase, should I use disposable blades, > cleaned with Rnase away? > 4) where can I find Rnase free slides, or should I just make my own. I > usually use charged slides. > > Any and all suggestions will be appreciated. I'm new to this and don't know > where I will have problems. > > Thanks! > > Caroline > > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 13 Date: Tue, 6 Jul 2010 22:10:44 -0400 From: Amos Brooks <[email protected]> Subject: Re: [Histonet] bat wing histology To: Mohit Chadha <[email protected]> Cc: [email protected] Message-ID: <[email protected]> Content-Type: text/plain; charset=ISO-8859-1 Hi, We did this on derm punch biopsies for a while. We used PLP (paraformaldehyde-lysine periodate) fixative. This was also done on a sliding microtome at 50um with piles of dry ice to keep the sucrose frozen. It was a bit of a pain in the butt, but we got decent results. I think the sectioning process could have been easier, but the PI was obsessed with not modifying the project at all, even if it meant improvements. We stained these as floating sections in 96 well plates, so in this case I would be concerned that the wing rolls would un-roll if you were to do the swiss roll thing I described. Have fun :-) Amos On Tue, Jul 6, 2010 at 5:10 PM, Mohit Chadha <[email protected]> wrote: > Thank you everyone for replying, much appreciated. > > Having also talked to people in my dept, I have a rudimentary protocol > ready. Of course, I will have to tweak it to see what works. I will be using > anti PGP9.5 antibody for neuronal immunology. > > I am still not sure how to section the wing. In most likelihood, I will be > using freezing sliding microtome. The "swiss roll" method sound good and I > will definitely try it. I am thinking that since the wing membrane is thin > (~30 um), I will also try to use the whole mount of small pieces. > > Any other thoughts and advice would be appreciated. > > Thank you, > Mohit Chadha, > Univ of Maryland. > > > > > > > > On Fri, Jul 2, 2010 at 4:14 PM, Amos Brooks <[email protected]> wrote: > >> Hi, >> I do hope you are looking at cross sections of the wing and not the >> flat. That would be very difficult indeed. For good cross sections I would >> try a "Swiss Roll". This is a way of demonstrating a large amount of cross >> sectional area in small space. Take the membrane and fix it by submersion in >> the fixative of your choice. Prior to processing roll the whole membrane up >> then cut the membrane log into sections small enough to fit in a cassette. >> You can use foam biopsy pads to support this shape. Embed it and section it >> on edge to show a long coiled membrane. The hairs should be able to be >> displayed in this way as well. To show a lot of membrane at the same time >> you could place multiple rolls in one cassette. This should work well. >> >> Good Luck, >> Amos >> >> >> Message: 21 >> Date: Thu, 1 Jul 2010 11:46:17 -0400 >> From: Mohit Chadha <[email protected]> >> Subject: [Histonet] bat wing histology >> To: [email protected] >> Message-ID: >> <[email protected]> >> Content-Type: text/plain; charset=ISO-8859-1 >> >> Hello everyone, >> >> This is my very first post and I am desperately looking for help. I am new >> to histology, so any help would be much appreciated. >> >> I am studying the peripheral sensory innervation of bat wings. As a first >> step, I would like to demonstrate the innervation pattern on the different >> parts of the wing membrane (a whole mount of the wing?). Second, I would >> like to demonstrate the mechanoreceptor make-up of the tiny hairs on the >> wing membrane. >> >> Bat wings are highly elastic, with numerous folds, a thickness of about >> 35-45 microns (in the species I study), a network of thin collagen >> bundles, >> and pigmented superficial epidermal layers. >> I could provide more information if required. >> >> Hoping to hear back from the members. >> Thank you. >> > > ------------------------------ Message: 14 Date: Tue, 6 Jul 2010 19:11:14 -0700 From: "Jennifer Campbell" <[email protected]> Subject: [Histonet] Has anyone used a biotin block in their antibody diluent? To: <[email protected]> Message-ID: <5658cbdb9eae6545abe50d2563d81bf8181...@vdxserver01.vdxpathology.local> Content-Type: text/plain; charset="iso-8859-1" Hi All, Has anyone ever used a Biotin block in their primary anitbody diluent? I have been having problems with nonspecific staining, which I suspect is due to endogenous biotin. I plan on decreasing my antigen retrieval time, as someone has told me that an antigen retrieval that is too vigorous may cause the unmasking of biotin, and its subsequent staining. I would like to know if anyone has had any luck using a biotin block in their diluent because I may try that as well. Thanks, Jennifer Campbell ------------------------------ Message: 15 Date: Wed, 7 Jul 2010 09:05:17 +0100 From: "Jim Reilly" <[email protected]> Subject: [Histonet] Biotin block To: <[email protected]> Message-ID: <fcd2109433379347b248142cae948edfcc8...@exchange-be6.centre.ad.gla.ac.uk > Content-Type: text/plain; charset="us-ascii" Hello Jennifer I use the Avidin/Biotin blocking kit from Vector SP-2001 I mix the Avidin D with my normal blocking serum and the biotin I add to my primary antibody diluent. This seems to work well for most tissue types. Cheers Jim ------------------------------ Message: 16 Date: Wed, 7 Jul 2010 07:32:52 -0400 From: "Mauger, Joanne" <[email protected]> Subject: [Histonet] RE: Has anyone used a biotin block in their antibody diluent? To: Jennifer Campbell <[email protected]>, "[email protected]" <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset="us-ascii" Jennifer, Vector has an avidin-biotin blocking kit that works well by itself or mixed with reagents- very reasonable cost. Jo Joanne Mauger HT(ASCP)QIHC Children's Hospital of Philadelphia ________________________________________ From: [email protected] [[email protected]] On Behalf Of Jennifer Campbell [[email protected]] Sent: Tuesday, July 06, 2010 10:11 PM To: [email protected] Subject: [Histonet] Has anyone used a biotin block in their antibody diluent? Hi All, Has anyone ever used a Biotin block in their primary anitbody diluent? I have been having problems with nonspecific staining, which I suspect is due to endogenous biotin. I plan on decreasing my antigen retrieval time, as someone has told me that an antigen retrieval that is too vigorous may cause the unmasking of biotin, and its subsequent staining. I would like to know if anyone has had any luck using a biotin block in their diluent because I may try that as well. Thanks, Jennifer Campbell _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Wed, 7 Jul 2010 08:37:27 -0500 From: [email protected] Subject: Re: [Histonet] used histology equipment To: <[email protected]> Cc: 'histonet' <[email protected]>, [email protected] Message-ID: <of32be6eed.da4838b0-on86257759.004aab55-86257759.004ad...@bhcpns.org> Content-Type: text/plain; charset="US-ASCII" Hi Diane, Because of your location I would recommend Micro-optics of Florida. Mike Jones or Tom Christy. The number is 954-791-0082. They are great people to work with and I have found them very reasonable on their prices. Hope this helps! Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 <[email protected]> Sent by: [email protected] 07/06/2010 05:29 PM To "'histonet'" <[email protected]> cc Subject [Histonet] used histology equipment Does anyone know of a reputable dealer for used equipment? _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. ------------------------------ Message: 18 Date: Wed, 7 Jul 2010 08:40:37 -0500 From: "Douglas,Joseph" <[email protected]> Subject: RE: [Histonet] used histology equipment To: 'Drew Meyer' <[email protected]>, "[email protected]" <[email protected]> Cc: histonet <[email protected]> Message-ID: <dacc27f195e63d44bdf7adc5c52051af1f02004...@dcpwvmbxc0vs1.mdanderson.edu > Content-Type: text/plain; charset="us-ascii" REMOVE FROM DATABASE -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Drew Meyer Sent: Tuesday, July 06, 2010 5:56 PM To: [email protected] Cc: histonet Subject: Re: [Histonet] used histology equipment Absolutely... Southeast Pathology Instrument Service out of Charleston, SC. The owner's name is Michael Dietrich. I've done business with him before and they are great people, very honest and they stand behind their instruments. I would highly recommend them to anyone. Contact Michael directly and tell him Drew Meyer from Atlanta referred you. http://southeastpathology.com/ Drew On Tue, Jul 6, 2010 at 18:29, <[email protected]> wrote: > Does anyone know of a reputable dealer for used equipment? > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Wed, 7 Jul 2010 08:41:59 -0500 From: "Douglas,Joseph" <[email protected]> Subject: RE: [Histonet] used histology equipment To: "'[email protected]'" <[email protected]>, "[email protected]" <[email protected]> Cc: 'histonet' <[email protected]>, "[email protected]" <[email protected]> Message-ID: <dacc27f195e63d44bdf7adc5c52051af1f02004...@dcpwvmbxc0vs1.mdanderson.edu > Content-Type: text/plain; charset="us-ascii" REMOVE FROM DATABASE -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of [email protected] Sent: Wednesday, July 07, 2010 8:37 AM To: [email protected] Cc: 'histonet'; [email protected] Subject: Re: [Histonet] used histology equipment Hi Diane, Because of your location I would recommend Micro-optics of Florida. Mike Jones or Tom Christy. The number is 954-791-0082. They are great people to work with and I have found them very reasonable on their prices. Hope this helps! Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 <[email protected]> Sent by: [email protected] 07/06/2010 05:29 PM To "'histonet'" <[email protected]> cc Subject [Histonet] used histology equipment Does anyone know of a reputable dealer for used equipment? _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Wed, 07 Jul 2010 08:36:47 -0700 From: [email protected] Subject: [Histonet] Reprocess To: [email protected] Message-ID: <20100707083647.9e2d9aa830e8449a2412eb1e4f2f067e.ed952ac3ac....@email04. secureserver.net> Content-Type: text/plain; charset="utf-8" Hello all!! Hope everyone had a happy 4th!! Question grossed in some fat that I need to routine process. I ha this in the past with extra fixation and no problem? This tim however it didn't fix all the way through and I have oily unfixed fat in section we longer and rep then what? older =) Thanks in advance!! Sarah Goebel, B.A., HT ( Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 ------------------------------ Message: 21 Date: Wed, 7 Jul 2010 11:47:39 -0400 From: Catherine Simonson <[email protected]> Subject: Re: [Histonet] Reprocess To: [email protected] Cc: [email protected] Message-ID: <[email protected]> Content-Type: text/plain; charset=ISO-8859-1 Easy option is to melt down, and run cassettes through the clean cycle on your processors to remove all the paraffin. then process as usual. I've done this before with decent results. Good luck! Catherine On Wed, Jul 7, 2010 at 11:36 AM, <[email protected]> wrote: > > Hello all!! Hope everyone had a happy 4th!! Question today is...I > grossed in some fat that I need to routine process. I ha ve done > this in the past with extra fixation and no problem? This tim e > however it didn't fix all the way through and I have oily unfixed fat > in the middle of my block. I fixed for 48 hours, but guess my > section we re too big and needed more. I want to fix for a little > longer and rep rocess the block. I know I need to melt it down, but > then what? I have done this before, it's just my brain is getting > older =) > > Thanks in advance!! > > Sarah Goebel, B.A., HT ( ASCP) > > Histotechnician > > XBiotech USA Inc. > > > 8201 East Riverside Dr. Bldg 4 Suite 100 > > Austin, Texas 78744 > > (512)386-5107 > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 22 Date: Wed, 7 Jul 2010 10:53:18 -0500 From: Cheri Miller <[email protected]> Subject: RE: [Histonet] Reprocess To: "[email protected]" <[email protected]>, "[email protected]" <[email protected]> Message-ID: <e3c81a010935ea41b379ac765103f3bf32c00e2...@olsrv12> Content-Type: text/plain; charset="us-ascii" Hi Sarah, I don't melt them down I just drop them in with my dirty molds and lids and run them through the cleaning cycle on my processor. The Xylene and Alcohol will melt the oily fat. When the clean cycle is complete I just drop them back into formalin until I process that evening. It works very well. Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of [email protected] Sent: Wednesday, July 07, 2010 10:37 AM To: [email protected] Subject: [Histonet] Reprocess Hello all!! Hope everyone had a happy 4th!! Question =day is...I grossed in some fat that I need to routine process. I ha= done this in the past with extra fixation and no problem? This tim= however it didn't fix all the way through and I have oily unfixed fat in =e middle of my block. I fixed for 48 hours, but guess my section we= too big and needed more. I want to fix for a little longer and rep=cess the block. I know I need to melt it down, but then what? =ave done this before, it's just my brain is getting older =) Thanks in advance!! Sarah Goebel, B.A., HT (=CP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ------------------------------ Message: 23 Date: Wed, 7 Jul 2010 11:01:37 -0500 From: Cheri Miller <[email protected]> Subject: RE: [Histonet] Reprocess To: Cheri Miller <[email protected]>, "[email protected]" <[email protected]>, "[email protected]" <[email protected]> Message-ID: <e3c81a010935ea41b379ac765103f3bf32c00e2...@olsrv12> Content-Type: text/plain; charset="us-ascii" I take that back I do melt them, recap the cassettes and then drop them in to clean. Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Cheri Miller Sent: Wednesday, July 07, 2010 10:53 AM To: [email protected]; [email protected] Subject: RE: [Histonet] Reprocess Hi Sarah, I don't melt them down I just drop them in with my dirty molds and lids and run them through the cleaning cycle on my processor. The Xylene and Alcohol will melt the oily fat. When the clean cycle is complete I just drop them back into formalin until I process that evening. It works very well. Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of [email protected] Sent: Wednesday, July 07, 2010 10:37 AM To: [email protected] Subject: [Histonet] Reprocess Hello all!! Hope everyone had a happy 4th!! Question =day is...I grossed in some fat that I need to routine process. I ha= done this in the past with extra fixation and no problem? This tim= however it didn't fix all the way through and I have oily unfixed fat in =e middle of my block. I fixed for 48 hours, but guess my section we= too big and needed more. I want to fix for a little longer and rep=cess the block. I know I need to melt it down, but then what? =ave done this before, it's just my brain is getting older =) Thanks in advance!! Sarah Goebel, B.A., HT (=CP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ------------------------------ Message: 24 Date: Wed, 7 Jul 2010 11:24:51 -0500 From: "Mahoney,Janice A" <[email protected]> Subject: RE: [Histonet] Reprocess To: "'[email protected]'" <[email protected]>, "[email protected]" <[email protected]> Message-ID: <[email protected]> Content-Type: text/plain; charset="utf-8" Cheri's process is a good one, we use it too. Works every time. Jan Mahoney Omaha, NE -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of [email protected] Sent: Wednesday, July 07, 2010 10:37 AM To: [email protected] Subject: [Histonet] Reprocess Hello all!! Hope everyone had a happy 4th!! Question =day is...I grossed in some fat that I need to routine process. I ha= done this in the past with extra fixation and no problem? This tim= however it didn't fix all the way through and I have oily unfixed fat in =e middle of my block. I fixed for 48 hours, but guess my section we= too big and needed more. I want to fix for a little longer and rep=cess the block. I know I need to melt it down, but then what? =ave done this before, it's just my brain is getting older =) Thanks in advance!! Sarah Goebel, B.A., HT (=CP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ------------------------------ _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 80, Issue 7 *************************************** _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
