** Proprietary ** ** Reply Requested When Convenient ** Hi there,
I was wondering if anyone on the histonet list use anti-mLYVE-1 antibody (purified in rat Monoclonal IgG 2A from R&D System) on the Bond Max. We have been trying out various titrations and pre-treatment without much success. All we seems to achieve is background staining with no real specificity at 1:100 H1(10) ; 1:100 H2(20) ;1:100 E2(10): 1:50 E1(30) ;1:50 H1(30); 1:50 H2(10) Any suggestion ? Regards Malika Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829 7875 ben...@gosh.nhs.uk ********************************************************************************************************* This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. ********************************************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
