Anything using FITC is pretty expensive. If you aren't want= ing flourescence then why would you use this? Does this specific conj ugation eliminate the Fc binding? Conjugating to HRP doesn't seem to = yield that Fc region staining that I can see? Just a happy Monday que= stion. =)
Sarah Goebel, B.A., HT (ASCP) Histotechnician <= /span> XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 = Suite 100 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: Re: [Histonet] human on human IHC From: [1]andreahoo...@rocketm= ail.com Date: Mon, July 19, 2010 6:06 am To: [2]sgoe...@xbiotech.com, "Ki= m Merriam" <[3]kmerriam2...@yah oo.com> Cc: "Histonet" <[4]h= isto...@lists.utsouthwestern.edu> Along those same lines I have conjugated primaries to biotin and it works b= eautifully for human on human IHC. Another alternative if extra amplificati= on is needed is to conjugating to FITC and then using a rabbit-anti-FITC fo= llowed by an anti-rabbit polymer. Just be aware with human on human controls are imperative as you may get bi= nding to human Fc receptors. Andrea Hooper Sent from my Verizon Wireless BlackBerry -----Original Message----- From: <[5]sgoe...@xbiotech.com> Sender: [6]hist= onet-boun...@lists.utsouthwestern.edu Date: Thu, 15 Jul 2010 09:27:19 To: Kim Merriam<[7]kmerriam2003= @yahoo.com> Cc: Histonet<[8]hist= o...@lists.utsouthwestern.edu> Subject: RE: [Histonet] human on human IHC I am doing alot with this right now. The best thing I havefound to do is to conjugate your primary antibody with HRP, then just use the direct method (no secondary), and it turns out beautifully!! You can buy thekit to do this from Fisher. I can get the exact order number if you need it. Good Luck!! Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: [Histonet] human on human IHC From: Kim Merriam <[1][9]kme= rriam2...@yahoo.com> Date: Thu, July 15, 2010 8:00 am To: Histonet <[2][10]histo...@lists.utsouthwestern.edu> Hi, Does anyone know of a human-on-human IHC kit similar to the mouse-on-mouse kits that are available? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list [3][11]histo...@lists= .utsouthwestern.edu [4][12]http://lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"[13]mailto://kmerriam2...@= yahoo.com"/ 2. 3D"[14]mailto://hi= sto...@lists.utsouthwestern.edu"/ 3. 3D"[15]mailto://Hi= sto...@lists.utsouthwestern.edu"/ 4. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"; _______________________________________________ Histonet mailing list [16]histo...@lists.utsou= thwestern.edu [17]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto://andreahoo...@rocketmail.com"/ 2. 3D"mailto://sgoe...@xbiotech.com"/ 3. 3D"mailto://kmerriam2...@yahoo.com"/ 4. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 5. 3D"mailto://sgoe...@xbiotech.com"/ 6. 3D"mailto://histonet-boun...@lists.utsouthwestern.edu"/ 7. 3D"mailto://kmerriam2...@yahoo.com"/ 8. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 9. 3D"mailto://kmerriam2...@yahoo.com"/ 10. 3D"mailto://histo...@lists.utsouthwestern.ed=/ 11. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 12. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"; 13. 3D"mailto://kmerriam2...@yahoo.com"/ 14. 3D"mailto://histonet@lists.utsouthwestern.edu"/ 15. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 16. 3D"mailto://Histonet@lists.utsouthwestern.edu"/ 17. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"; _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
