I use FisherBrand SuperFrost Plus charged slides, the only time I have this problem is when my waterbath is too hot, or if for some reason I use warmed slides to retrieve my sections from the bath (like if you lay the unused slides on the edge of the waterbath, or if you use the warm droplet method to spread your sections)? I haven't had much experience with a lot of different slide types/brands though, but it might be an easy fix to the problem you hadn't thought about, as it is more or less unrelated to the brand of slide, although some brands may be more sensitive to this issue...
Sincerely, Nicole Collette Lawrence Livermore National Lab/ UC Berkeley -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of nap...@mail.siscom.net Sent: Friday, July 23, 2010 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water collecting at bottom of sections Hello all, >From time to time and depending on what brand of adhesive (or charged) slides I am using, I seem to get a "bag" of water that drains to the bottom of my sections but doesn't drain "out". I have been working in microtomy a long time and have had to deal with this contingency time and time again, but never really have gotten to the bottom of the problem. I spoke with a premium manufacturer of such slides and they seemed to indicate that it is a problem with the coating, but couldn't tell me for sure. All I know is that certain brands do this more than others. If you know what I mean, you know it is a problem. My bath is pure distilled H2O with no gelatin or Sta-on added. It is if the adhesive properties are SO good that they will not release the water when vertically drained and have to be shaken off or cut with a razor blade at bottom to release the water. Anyway, if anyone has an insight or two on this, I would be interested. It seem sthe most challenging issues are ones that seem related to some of the most simple tasks that one has performed for many years!! Manufacturers understand what I mean, but cannot pinpoint the problem for me via phone or e-mail. Anyone see this and have a chemical/mechanical solution they have developed over the years? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://*lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
