To All: We have been experiencing problems sectioning mouse brain. The sectioning is fine, but we have problems in the water bath. At 45 degrees C (peel-a-way paraffin-Polysciences), the sections break apart but we don't get wrinkles. With paraplast extra, we use 36 degrees C, but the sections are wrinkled and disintegrate. I would appreciate hearing from listers as to what you do: what temperature is your water bath, what embedding media do you use, etc? Any help would be appreciated! Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org
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