Hello all, I have received embolic material in test tubes in 10% formalin. The tubes had been sitting around for about 2 years. To quantitate the number of embolic particles, after removing particles at less than 40 microns, the solution was poured into a petri dish and then scanned. However, in about 1/3rd of the test tubes, a hard disk of what looks like red blood cells formed on the bottom when the RBCs should have been suspended in solution. These hard discs (scabs) were broken up and also included in the petri dish scans. The problem is that the scabs should not be measured with the other particles because these weren't measured in a previous study: the RBCs in a previous study did not coagulate. So now I have to figure out which of the broken up scabs are scabs and which could be other embolic material. In the past, I irradiated the RBCs with green light and collected through a red filter and this revealed RBC laden particles. However, these aren't autofluorescing, possibly because they have been in solution so long (or because the scabs are a mix of embolic materials). Here's the million dollar question: has anyone had to identify scabs on a macro level, and how was that done? I know all the embolic material can be spun down and sectioned, but the idea is to get the quantitative information about the nature of the particles BEFORE spinning down. Thanks! Jerry
-- Jerry (Gerald) Sedgewick Author: "Scientific Imaging with Photoshop: Methods, Measurement and Output." Sedgewick Initiatives 965 Cromwell Avenue Saint Paul, MN 55114 651-788-2261 [1]jerrysedgew...@gmail.com [2]http://www.quickphotoshop.com [3]http://www.rawlight.com [4]http://www.jerrysedgewick.com References 1. mailto:jerrysedgew...@gmail.com 2. http://www.quickphotoshop.com/ 3. http://www.rawlight.com/ 4. http://www.jerrysedgewick.com/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet