There is an OCT for lower temperatures. ----- Original Message ----- From: "Della Speranza, Vinnie" <del...@musc.edu> To: "Bruce W Brodersen" <bbrod...@unlnotes.unl.edu>, histo...@pathology.swmed.edu Sent: Monday, August 9, 2010 9:40:03 AM Subject: RE: [Histonet] friable or crumbly O.C.T.
I'm guessing that liquid nitrogen or dry ice temperature is too cold for sectioning OCT. OCT cuts well down to about -25 degrees C. Liquid Nitrogen is about -160 degrees C. I believe dry ice is in the same temp range at LN2 You will want to give the OCT blocks the opportunity to "warm up" to cryostat temperature before attempting to section them. Leave your frozen blocks in the cryostat for 30-60 minutes before sectioning to allow them to come to optimum temperature Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bruce W Brodersen Sent: Friday, August 06, 2010 2:26 PM To: histo...@pathology.swmed.edu Subject: [Histonet] friable or crumbly O.C.T. Anyone have an explanation as to why OCT would be friable or crumbly for sectioning? Here's how it was used. Thanks. "We held the plastic 'tray' with the tissue in the compound just over the liquid nitro for 30sec-1min, until it was opaque and white (frozen) and then dipped the tray into the liquid nitro for 20-30sec., placed in small bags and then into a cooler with dry ice until shipping." Bruce W. Brodersen, DVM, PhD University of Nebraska Veterinary Diagnostic Center 1900 N. 42nd Street Lincoln, NE 68583-0907 voice (402) 472-1434 FAX (402 472-3094_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
