OMG. This problem was ever present in our lab for years, until I figured out that ANY minute trace of moisture, water, eosin in the last absolute alcohol would interfere with the activation of the adhesive in the tape, and create this brown artefact. We religiously change our absolute alcohols on the autostainer so the last alcohol is perfectly clear of any hint of eosin, and the artefact has disappeared from our tape-coverslipped slides. The Sakura rep couldn't help me either, and told me they had NEVER seen this problem before. Love it.
Keep your last 100% PRISTINE, and the artefact will be eliminated. I promise. Jackie O' From: "Nails, Felton" <[email protected]> To: "'Gauch, Vicki'" <[email protected]>, "[email protected]" <[email protected]> Date: 08/11/2010 01:45 PM Subject: [Histonet] Sakura Tape coverslipper Sent by: [email protected] Has anyone been experiencing a brown cornflaking artifact on their slides after being coverslipped. I was told by Sakura that it was do to the slides drying out when transferred from the stainer to the coverslipper. Which can't be correct because on the new sakura coverslipper there is a holding area which is filled with xylene so if the tissue dried out it would rehydrate before being coverslipped. ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
