Key Kim,
The blade angle depends on the manufacturer of the microtome. In general,
Leica suggests and angle of 3 to 5. Microm uses 10 to 12 and Shandon uses
0 to 3. Confused? Me too and I repair them for a living.

I have found that vibration is caused by problems with the knife holder
more often than knife angle. The problem can be as simple as the pressure
plate not being tight enough. Let me know what type of tome you have and I
may me able to help.

Best
Matt Mincer
Tech One Biomedical
708-822-3738


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