Is this what you are looking for? Ng et al Re-hydration Method for Red Blood Cell Lysis
Principle: R-ehydration of air dried smears with normal saline for 30 seconds before fixation in alcohol has been found to produce slides of a quality superior or at least equivalent to that of immediate fixed smears. It allowed the cells to adhere better to the slide and lysis of red blood cells provided a clean background for better assessment. Solutions: 1. Normal Saline Sodium Chloride 9g Distilled water 1000ml 2. 95% Ethanol Procedure: 1. After smearing material, air dry slides using a hair dryer. 2. Place slides in Normal Saline for 30sec. 3. Immediately place slides in 95% ethanol and fix for 10 minutes. 4. Stain slides via PAP. Results: RBCs were effectively lysed but epithelial and mesothelial cells were retained. In general, smears showed a decrease in chromaticity of both nuclear and cytoplasmic staining. Cytoplasmic vacuoles were less distinct and epithelial fragments, such as acini, appeared flattened and could be more easily focussed on the same plane. Nuclear and cellular moulding was exaggerated and more appreciable when present. There was a slight enlargement of cells. Degenerated specimens, even in the absence of polymorphs, often resulted in disappointing re-hydrated smears. Cell clusters were easier to see probably because of the higher transparency of the cytoplasm. Reference: Acta Cytolog (1994) 38(1):56-64. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Aazath Raj Sent: Thursday, 12 August 2010 10:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 80, Issue 31 Dear Friends, Does anyone have rehydration technique of air dried smear which can be stained for pap. Aazathraj.P Technical Officer Department of Histopathology and Cytology Apollo Hospitals -chennai India _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet