Cindy,

I have very little experience with the Technovit kits as I predominantly use a  
MMA + DBP + Perkadox formulation for both thin and thick section histology. 
However, in my experience with the Technovit kits, I have discovered for me 
that the MMA + DBP + Perkadox type of formulation is more consistent, 
reproducible and overall more flexible for all types of microtomy applications 
and also for the variety of species (mouse to human) of bone tissue. I have 
more control over the quality of resin block I can produce with an MMA + DBP + 
Perkadox formulation, thin sections can be deplastified and staining is more 
routine and flexible.

As for mounting thin microtomed sections, you need to coat your slides with 
some type of adhesive so that your sections will stay mounted throughout 
staining. I use Haupt's adhesive to coat my glass slides, along with an 
aluminum slide press and oven to activate the Haupt's media and complement the 
adhesion process. There is a step by step process that I can share with you if 
interested to help accomplish the section adhesion.

Now with regards to cracking/crazing of ground (thick) sections in the presence 
of certain stains. I believe the best way to describe this is that these 
experiences are proportional to the density or hardness of the resin. If you 
have a hard resin section (based upon the overall hardness of you resin block), 
some chemicals will act to make the section brittle and crack similar to 
prolonged use of xylenes with undeminerized bone processing or prolonged 
paraffin infiltration is a soft tissue application.

Jack

On Aug 24, 2010, at 7:57 PM, C B <clb1...@yahoo.com> wrote:

> Anyone using Technovit 9100 for microtome and/or ground sections?  Do you use 
> the routine Plus slides for mounting microtome sections or do the sections 
> require another type of adhesive?  When staining the ground sections,  do you 
> have any problems with certain solutions causing cracking/crazing?
> Cindy Baranowski, HT (ASCP)
> Saint Joseph's Translational Research Institute
> Atlanta, Ga
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Reply via email to