yeah, I'd be careful of this method. We used to do this at the U.S. Air
Force hospital I used to work at when I was active duty. One time, the fire
alarm went off and we had to evacuate the lab. Well, there must have been a
real problem because we were out of the lab a long time. By the time we got
the "all clear" to go back into the lab, I was the first one back and ran
into some firemen. Seems the water boiled out of the pot and the paraffin
was smoking up a storm.. No one said anything, but I bet that was why we
were out of the lab so long. Now, those of you that have worked in a
military or government hospital realize all the freaking paperwork that had
to be filled out from that one incident. We were ordered by Safety to
develop another method. So, we soak our molds in xylene for a couple of
hours, covered of course as not expose the lab to the xylene fumes (that
would required addition freaking paperwork) followed by a 100% etoh rinse,
followed by air drying.
JTT
----- Original Message -----
From: <cha...@yahoo.com>
To: "Webb, Dorothy L" <dorothy.l.w...@healthpartners.com>;
<histonet-boun...@lists.utsouthwestern.edu>;
<histonet@lists.utsouthwestern.edu>
Sent: Thursday, August 26, 2010 11:16 AM
Subject: Re: [Histonet] Cleaning molds
Back when I was using metal cassette lids and an autotechnicon (so no
cleaning in the processor) I would throw all the lids and molds into a pot
after embedding. Throw a bit of laboratory detergent in, fill with water,
and boil on a hot plate. Once I got to a rolling boil, I would turn the
heat off. By the end of the day it was cool. Peel the paraffin off the
top, then rinse the molds.
Worked great! Didn't even need mold release.
Will
Sent from my Verizon Wireless BlackBerry
-----Original Message-----
From: "Webb, Dorothy L" <dorothy.l.w...@healthpartners.com>
Sender: histonet-boun...@lists.utsouthwestern.edu
Date: Thu, 26 Aug 2010 10:53:58
To: 'histonet@lists.utsouthwestern.edu'<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Cleaning molds
Does anyone in histoland clean their embedding molds in a dishwasher?
Otherwise, besides placing in the cleaning cycle of your processor, how do
sites clean their molds?? Simple, but plaguing question!!!!!!! Thanks
all!
Dorothy Webb
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