Omit the primary antibody, or you can always buy a "negative cont rol". It does the same thing as just incubating the section in buffer= for the same time as the positive control with the primary (and it saves a= ton of money!). If you need a specific negative control tissue, this= is going to be different for every antibody.
= Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas = ; 78744 (512)386-5107 -------- Original Message -------- Subject: Immunofluorescence QC From: "Nails, Felton" <[1]fln= a...@texaschildrens.org> Date: Tue, August 31, 2010 10:26 am To: "'[2]sgoe...@xbiotech.com'" &l= t;[3]sgoe...@xbiotech.com>, "Ch akib Boussahmain" <[4]chak_...@yahoo.com> Cc: "[5]histo...@lists.ut= southwestern.edu" <[6]histo...@lists.uts= outhwestern.edu> We have always done IF's utilizing internal positive controls. The new CAP = checklist is now requiring positive and negative controls. What are you guy= s doing for negative controls? ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ======================= 3D= ======================= 3D== ========== References 1. 3D"mailto:flna...@texaschildrens.org"; 2. 3D"mailto:sgoe...@xbiotech.com"; 3. 3D"mailto:sgoe...@xbiotech.com"; 4. 3D"mailto:chak_...@yahoo.com"; 5. 3D"mailto:histonet@lists.utsouthwestern.edu"; 6. 3D"mailto:histonet@lists.utsouthwestern.edu"; _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
