Hello, I have been asked to to prepare frozen sections of fish livers that have been stored in RNAlater Solution at -80C. Tissues (about 10 um square x 5 um thick) were embedded in OCT, frozen on dry ice, and sectioned at 6 um. My problem is that when I cut a section, the center of the tissue is missing! Approximately 1 mm of tissue around the edges seems to section just fine, but then there is just a gaping hole in the center as if the tissue is receding while it is being cut.
I've tried sectioning at various temperatures (-20C, -16C, and -12C) and thickness (4um-8um). Any help will be greatly appreciated, Carla Conway Western Fisheries Research Center Seattle, WA ph: 206-526-6282 ext. 242 cmcon...@usgs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
