Hello all, I have a request to carry out a combined staining with H&E + Alcian Blue pH2.5. I have tried in vain to get this to work but regardless of the order of staining the end result is dark blue/purple mucin. I have carried out a parallel experiment whereby the staining has been checked microscopically then stopped after each of the dyes. (Thus giving 3 slides stained with: AB, AB+H and AB+H+E) The AB and AB+H are really nicely stained but as soon as the eosin is added (using 2 different stains and a variety of times) the mucin staining looks similar to the nuclear stain. I am expecting the alcian blue to remain turquoise: should it?
For information, I am trying to stain salmon intestine at 5 microns for using the following: Alcian Blue 8GX (certified), pH has been checked Haematoxylin Z (Cellpath Uk) 1% aq Eosin (Cellpath uk) and lab prepared solution from dye. Thanks from a frustrated technician! Debbie Faichney Histopathology Institute of Aquaculture University of Stirling Scotland UK -- The Sunday Times Scottish University of the Year 2009/2010 The University of Stirling is a charity registered in Scotland, number SC 011159. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
