Any suggestions for staining nets for floating brain sections? I've looked at netwells, cell strainers and have even considered making my own. Most commercial options are way too expensive. I'd love to hear what everyone uses and if there is an easy way to make them.
Thanks! On Sep 12, 2010, at 1:01 PM, "histonet-requ...@lists.utsouthwestern.edu" <histonet-requ...@lists.utsouthwestern.edu> wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Reichert Frigocut 2800 Manuals (ricky hachy) > 2. Larry Farrand is out of the office (larry_farr...@vwr.com) > 3. Re: Flat Ice??? (tracz...@aol.com) > 4. RE: Flat Ice??? (histot...@imagesbyhopper.com) > 5. Sakura X50 Microwave Tissue Processor (Carlos Hernandez) > 6. Trouble shooting decalcified bone sections (paraffin > embedded) (CHEN, YIJING) > 7. Re: Sakura X50 Microwave Tissue Processor (Rene J Buesa) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sat, 11 Sep 2010 17:25:28 +0000 > From: ricky hachy <elc...@hotmail.com> > Subject: [Histonet] Reichert Frigocut 2800 Manuals > To: Histonet <histonet@lists.utsouthwestern.edu> > Message-ID: <snt114-w49cbd4700d7e1e972a577bcf...@phx.gbl> > Content-Type: text/plain; charset="iso-8859-1" > > > Hello, > > Does anybody have the user or service manuals for the RIECHERT-JUNG FRIGOCUT > 2800 ? > > Thanks > Ricky > > ------------------------------ > > Message: 2 > Date: Sat, 11 Sep 2010 14:01:07 -0400 > From: larry_farr...@vwr.com > Subject: [Histonet] Larry Farrand is out of the office > To: histonet@lists.utsouthwestern.edu > Message-ID: > <of8f0b02c4.128765ea-on8525779b.0062fab1-8525779b.0062f...@vwr.com> > Content-Type: text/plain; charset=US-ASCII > > > I will be out of the office starting 09/10/2010 and will not return until > 09/20/2010. > > I will not have access to e-mail. Please contact customer service with any > questions. If you have an issue that needs to be addressed, Karen Longo my > regional manager can be contacted to assist you (karen_lo...@vwr.com or > 1_847-412-8900) > > > > > ------------------------------ > > Message: 3 > Date: Sat, 11 Sep 2010 19:21:27 EDT > From: tracz...@aol.com > Subject: Re: [Histonet] Flat Ice??? > To: sraib...@yahoo.com > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <46abb.58819722.39bd6...@aol.com> > Content-Type: text/plain; charset="US-ASCII" > > I had the same problem. Now I make sure the pan is covered when I put it > in the freezer. I just use a stray plastic container cover and it works > out just fine. > Dorothy > > > ------------------------------ > > Message: 4 > Date: Sat, 11 Sep 2010 22:22:50 -0400 > From: <histot...@imagesbyhopper.com> > Subject: RE: [Histonet] Flat Ice??? > To: "'WILLIAM DESALVO'" <wdesalvo....@hotmail.com>, > <msherw...@partners.org>, <sraib...@yahoo.com>, "'histonet'" > <histonet@lists.utsouthwestern.edu> > Message-ID: <742760ba93f24a9b851a6a3376204...@hopperpc> > Content-Type: text/plain; charset=US-ASCII > > I much prefer the "wet ice" approach to the more dry variety of the cool > trays. The "wet" ice gets my blocks colder and I like the fact that it > introduces a small amount of moisture into the already faced block. My > blocks are not on the ice for long periods of time, so over-saturating them > with water is not an issue. > > To keep my trays from growing mountains, I have found that if I fill them up > 3/4 of the way and let them freeze and then follow up with the other 1/4 > later in the day, I get nice, flat little "ice rinks" that I can lay my > blocks on. It's not time consuming either, just have to remember to go back > and pour more water on them! By the way, this works on ice cube trays as > well as steel pans we have. I don't really care for the pans, but some of > my techs do. They take a little more work to make them ice rinks. ;o) > > My $0.02. :o) > > Michelle > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM > DESALVO > Sent: Friday, September 10, 2010 12:03 PM > To: msherw...@partners.org; sraib...@yahoo.com; histonet > Subject: RE: [Histonet] Flat Ice??? > > > > For quality assurance and safety concerns, I would suggest moving away from > the "wet ice" and go with a sealed freezing tray. We moved to the small > Histo-Cool ice trays w/ tray. > > http://www.labstore.com/catalog/index.cfm?Category_ID=375 > > William DeSalvo, B.S., HTL(ASCP) > > > > > >> Date: Fri, 10 Sep 2010 10:58:01 -0400 >> From: msherw...@partners.org >> To: sraib...@yahoo.com; Histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] Flat Ice??? >> CC: >> >> We use a styrofoam container and have no problem with it freezing >> flat. Are you containers sitting flat in the freezer? >> >> -----Original Message----- >> From: histonet-boun...@lists.utsouthwestern.edu >> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Susan >> Raibley >> Sent: Friday, September 10, 2010 10:52 AM >> To: Histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Flat Ice??? >> >> Hello! I would greatly appreciate any tips or tricks to getting ice to >> freeze flat in the pan! We are tired of opening the freezer to see >> mountains that have formed in our pans overnight, making it hard to >> try and fit all the blocks we need to microtome on them. We have two >> freezers and they both have this problem. Help please! Thanks! >> >> >> Susan Bincsik, HT (ASCP) >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> The information in this e-mail is intended only for the person to whom >> it is addressed. If you believe this e-mail was sent to you in error >> and the e-mail contains patient information, please contact the >> Partners Compliance HelpLine at http://www.partners.org/complianceline >> . If the e-mail was sent to you in error but does not contain patient >> information, please contact the sender and properly dispose of the >> e-mail. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > No virus found in this incoming message. > Checked by AVG - www.avg.com > Version: 8.5.441 / Virus Database: 271.1.1/3117 - Release Date: 09/08/10 > 06:07:00 > > > > > ------------------------------ > > Message: 5 > Date: Sat, 11 Sep 2010 20:57:11 -0700 (PDT) > From: Carlos Hernandez <c_m_hernande...@yahoo.com> > Subject: [Histonet] Sakura X50 Microwave Tissue Processor > To: histonet@lists.utsouthwestern.edu > Message-ID: <399458.78523...@web31804.mail.mud.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > I'm looking for some feedback (pros & cons) from anyone using the Sakura X50. > Especially any derm labs that have experience with it. > > Thanks, > > Carlos > > > > > ------------------------------ > > Message: 6 > Date: Sun, 12 Sep 2010 01:47:11 -0400 > From: "CHEN, YIJING" <ych...@kent.edu> > Subject: [Histonet] Trouble shooting decalcified bone sections > (paraffin embedded) > To: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: <c8b1e19f.840b%ych...@kent.edu> > Content-Type: text/plain; charset="us-ascii" > > Hi, > We are having difficulty sectioning paraffin-embedded decalcified adult > mouse autopods (paws). The tissues shatter as soon as they hit the blade > when sectioned. > > The autopods were soaked in CalEX for 4 days at room temp and felt extremely > soft before embedding, suggesting effective decalcification. We use the > Sturkey EXTREMUS low profile disposable knives on our microtome. These > knives are said to be coated with the hardest nitride coating available. > Any suggestions will be greatly appreciated. > Sincerely, > Yijing > > > > > ------------------------------ > > Message: 7 > Date: Sun, 12 Sep 2010 07:28:49 -0700 (PDT) > From: Rene J Buesa <rjbu...@yahoo.com> > Subject: Re: [Histonet] Sakura X50 Microwave Tissue Processor > To: histonet@lists.utsouthwestern.edu, Carlos Hernandez > <c_m_hernande...@yahoo.com> > Message-ID: <626067.66375...@web65716.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Just a rhetorical question: do you need such an expensive instrument to > process skin Bxs? > René J. > > --- On Sat, 9/11/10, Carlos Hernandez <c_m_hernande...@yahoo.com> wrote: > > > From: Carlos Hernandez <c_m_hernande...@yahoo.com> > Subject: [Histonet] Sakura X50 Microwave Tissue Processor > To: histonet@lists.utsouthwestern.edu > Date: Saturday, September 11, 2010, 11:57 PM > > > I'm looking for some feedback (pros & cons) from anyone using the Sakura X50. > Especially any derm labs that have experience with it. > > Thanks, > > Carlos > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 82, Issue 14 > ****************************************
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