Patrick: Under separate cover I am sending an article about NBF fixation and its comparison with other fixatives so you can make an "informed" decision. René J.
--- On Wed, 9/15/10, Lewis, Patrick <patrick.le...@seattlechildrens.org> wrote: From: Lewis, Patrick <patrick.le...@seattlechildrens.org> Subject: [Histonet] NBF fixation and long term storage To: Histonet@lists.utsouthwestern.edu Date: Wednesday, September 15, 2010, 1:53 PM I have some questions about Fixing tissues for paraffin embedding when the ultimate purpose will be looking for viral antigens and cell surface markers. We have some monkey tissue that has been in NBF for over a year. and we are just now processing them and embedding them in to paraffin. We will be getting additional tissues from monkey necropsies and in an effort to avoid epitope damage from prolonged exposure to NBF I was wondering if anyone could recommend a fixation period. (I.E. no longer than 24 hours in NBF, 48 hours? ETC. I was thinking of replacing the NBF (Neutral buffered Formalin) with 70% Etoh. And using 70% Etoh as long term storage of tissue parts. The ideal situation would be to get the tissues from necropsy the same day of the necropsy and store the tissues overnight in NBF and then transfer them to 70% etoh and then process them on the tissue processer after 24 hours in Etoh. I'm not sure how many animals I've eventually get and I'm a little concerned over where we will put all the spare animal parts that I'm sure I'll accumulate. We don't want to throw anything away and for some tissues I'll have more than I can put into a single cassette. I may end up using more than 1 cassette for a single piece of tissue so that I have backup cassettes of it. Anyone storing research tissues has a suggestion about the best way to store them when space is a premium. Thanks Patrick. Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000-0000 PAGER 000-000-0000 CELL 206-884-7311 FAX patrick.le...@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org <http://seattlechildrens.org/> CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet