Thanks, Montina The sections are 40um and I had no trouble with all my other brains (sectioned myself). I had help with these last two and they are all curled. I think they just got sectioned too fast or something. I'll try the shaker and see if it helps. Thanks!
-Teresa On Wed, Sep 15, 2010 at 8:47 PM, Montina Van Meter < montina.vanme...@pbrc.edu> wrote: > Teresa, > I would put the sections through several 5-10 min. washes on a shaker > table. It is very important to make sure you have all of the cryoprotectant > rinsed out of the tissue or it will inhibit IHC staining. How thick are the > sections? Were they cut on a cryostat or freezing microtome? I routinely > cut rat brain at 40um and don't have any curling issues. That sometimes > occurs when the knife has come through the section of brain too rapidly. A > slow and steady motion is needed when cutting frozens. > > Good luck! > Tina Van Meter > > > Sent from my iPhone > > On Sep 15, 2010, at 9:37 PM, "Teresa Iglesias" <tligles...@ucdavis.edu> > wrote: > > >> -- ______________________________ Teresa Iglesias Graduate Group in Animal Behavior Department of Evolution and Ecology University of California-Davis One Shields Avenue 2320 Storer Hall Davis, CA 95616 Office: 530-754-7837 Fax: 530-752-1449 tligles...@ucdavis.edu ______________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
