Hello Histonetters,
I have been using Picrosirius Red stain to detect collagen fibers in residues lifted from archaeological artefacts. The protocol has been adapted for staining slides rather than tissue samples however I am curious if it is normal that everything on the slide stains red. I realise that the collagen fibers can be easily detected in cross polarised and part polarised light ( yellow and orange = large fibers and green the thinner fibers and all are highly birefringent). While only the collagen changes colours in cross polarised light I am wondering if it is normal that everything on the slide picks up the stain to some extent and turns red (pink) when viewed in plane polarised light? This includes seed parts, plant lipids and ochre? Any thoughts or ideas? Thanks Regards Birgitta Stephenson Archaeological Microscopy Research Lab University of Queensland Birgitta Stephenson Pharmacist Advisor Veteran Affairs Pharmacy Advisory Centre Phone (07) 3223 8435 Fax (07) 3223 8651 ______________________________________________________________________ IMPORTANT 1. Before opening any attachments, please check for viruses. 2. This e-mail (including any attachments) may contain confidential information for the intended recipient. If you are not the intended recipient, please contact the sender and delete all copies of this email. 3. Any views expressed in this e-mail are those of the sender and are not a statement of Australian Government Policy unless otherwise stated. 4. Electronic addresses published in this email are not conspicuous publications and DVA does not consent to the receipt of commercial electronic messages. 5. To unsubscribe from emails from the Department of Veterans' Affairs (DVA) please go to http://www.dva.gov.au/contact_us/Pages/feedback.aspx , and advise which mailing list you would like to unsubscribe from. 6. Finally, please do not remove this notice. -- Birgitta Stephenson bstep...@fastmail.fm -- http://www.fastmail.fm - Or how I learned to stop worrying and love email again _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet