thanks Amos which one did you used ? you have catalog number ? On Fri, Oct 1, 2010 at 9:44 PM, Amos Brooks <amosbro...@gmail.com> wrote:
> Hi, > I am currently working on HMGB1. It is a weird one! If you go by the > data sheets you will get great nuclear staining on all the nuclei. This is > not what you are after if you have necrotic tissue. What you want to see is > the nuclei in necrotic tissue fade out and have the cytoplasm take it up. I > am cutting the dilution to 1: 50 and 1:100 since we were just starting to > see blushes of cytoplasmic staining and no nuclear label at higher > dilutions. Usually you get staining that fades out at higher dilutions. This > one was really odd. > > Good luck, > Amos > > > Message: 13 > Date: Thu, 30 Sep 2010 04:55:02 +0200 > From: Fabrice GANKAM <gan...@googlemail.com> > Subject: [Histonet] HMGB and RAGE; TLR2, TLR4 antibodies > To: <histonet@lists.utsouthwestern.edu> > Message-ID: <c4a46c631cd14a86aad3ce3abb29a...@pcdegankam> > Content-Type: text/plain; charset="us-ascii" > > > Hey guys > Just wanted to know if any of you had some luck with an antibody aigainst > rat HMGB1 and its receptors RAGE, TLR2, TLR4. > W > Thanks > Dr Fabrice GANKAM > UTSW > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
