Neil, You need to do some fixation. Hopefully they are mounted on charged slides.
We routinely post-fix fresh-frozen human brain sections in cold Acetone/ETOH (3:1) for 10 min and then proceed with the IHC staining. Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Neil M. Fournier Sent: Monday, October 04, 2010 3:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC unfixed human brain tissue I have limited experience working with human brain tissue and would like to stain some human brain sections that I received. They were not fixed, but were flash frozen and sectioned on a cryostat, and mounted onto slides. Could someone give me a brief run down of their standard methodological staining procedure with unfixed human brain sections? Much appreciated Neil _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet