Patsy,
Here is THE citation and abstract for this publication in J Histochem Cytochem (found it by Googling staining method). You can download the pdf at no charge. The photographs are excellent. Journal of Histochemistry and Cytochemistry Volume 52 (2): 211-216, 2004 Silver Carbonate Staining Reveals Mitochondrial Heterogeneity José M. LópezCepero Summary Silver staining methods, when selective, yield a high-contrast and high-resolution image in optical microscopy. A classical method for silver impregnation of mitochondria has been applied to murine tissues and reveals a marked heterogeneity among mitochondria in single cells. This heterogeneity can be detected in the optical microscope but is even more evident at the ultrastructural level. The differences in staining intensity may reflect different stages in the mitochondrial life cycle. The progressive accumulation of uranylargyrophilic material may be a marker of mitochondrial aging. This highly selective staining procedure may be of use in studies of mitochondrial changes under pathological conditions and during apoptosis. (J Histochem Cytochem 52:211216, 2004) Key Words: mitochondria silver carbonate mitochondrial life cycle mitochondrial heterogeneity mitochondrial fusion Gayle M. Callis HTL/HT/MT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet