When you place dye on any tissue, make sure you pipette some bouins on to the tissue... Apparently the bouins "sets" the stain... afterwards dab the tissue with paper towel to sop up the excess dye/bouins.
Some dyes are better than others, but try the bouins first Call me or email me directly, I can help you with details :) Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jacqueline Farnsworth Sent: Tuesday, October 05, 2010 2:14 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] embedding beads/tissue marking dyes Hi We may be encountering some issues with our tissue marking dyes 'clogging' up our processors. I"ve heard of a system where a coloured 'bead' is embedded right beside the tissue, and subsequently cut onto the slide. This does not mark the margins obviously, but is used as a method to track like specimens that are grossed, embedded and subsequently cut in a row. (red, orange, green, blue.....) I explored the archives for "embedding beads", but only found reference to a glass bead that is placed in the wax inside the block, but not subsequently cut. PS: we are still troubleshooting our dyes (dilutions, brand, etc.), but if anyone has a brand of dye that they like and have no issues with, I'd be thrilled to get the information as well! Thanks in advance, Jacquie Jacqueline Farnsworth Anatomic Pathology, Tech III Diagnostic Scientific Centre Calgary Laboratory Services Phone: 403-770-3588 Pager: 403-212-8223 X07630 P Please consider the environment before printing this email. ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
