Does anyone know of any GLP training in El Paso, TX? Kendra Leigh Carter -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, October 06, 2010 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 83, Issue 6
Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Helicobacter pylori (Laurie Colbert) 2. RE: AChE fiber staining protocoll? (szi...@bio.u-szeged.hu) 3. RE: Helicobacter pylori (Weems, Joyce) 4. PAS STAIN (Diana McCaig) 5. Histokinette 2000 (Biedermann, JoAnn) 6. RE: PAS STAIN (Rathborne, Toni) 7. Query Special Stainers (Joanne Clark) 8. Re: Helicobacter pylori (Brandi Higgins) 9. AW: [Histonet] PAS STAIN (Gudrun Lang) 10. Where to buy Michels RE: [Histonet] DIF Transport Media (gayle callis) 11. RE: Query Special Stainers (Mahoney,Janice A) 12. RE: PAS STAIN (gayle callis) 13. RE: Query Special Stainers (Marcia Funk) 14. PAS staining (Janet Keeping) ---------------------------------------------------------------------- Message: 1 Date: Wed, 6 Oct 2010 07:48:25 -0700 From: "Laurie Colbert" <laurie.colb...@huntingtonhospital.com> Subject: RE: [Histonet] Helicobacter pylori To: "Kathy M. Gorham" <km...@grh.org>, <histonet@lists.utsouthwestern.edu> Message-ID: <57be698966d5c54eae8612e8941d768309a5a...@exchange3.huntingtonhospital.com> Content-Type: text/plain; charset="us-ascii" We basically do a dif quik stain. I buy a kit called "Three Step Stain" from Cardinal. The total staining process takes about 1 minute. Laurie Colbert -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, October 06, 2010 7:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Helicobacter pylori Good Morning Histo land. I would like to know what others are using for a special stain for Helicobacter pylori not IHC. Do you use a kit? From where? Make up your own? Procedure. Thanks you. You have always been so helpful. Kathy Gorham H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 06 Oct 2010 17:00:35 +0200 From: szi...@bio.u-szeged.hu Subject: RE: [Histonet] AChE fiber staining protocoll? To: "Houston, Ronald" <ronald.hous...@nationwidechildrens.org> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <20101006170035.j2waztulso448...@webmail.u-szeged.hu> Content-Type: text/plain; charset=ISO-8859-2; DelSp="Yes"; format="flowed" Idézet ("Houston, Ronald" <ronald.hous...@nationwidechildrens.org>): Thank you for the answer. We will try this protocol and i will reply the results. Csaba Szigeti > I have had great success in fine fibers with the modification of > Martucciello et al, Eur J Pediatr Surg 2001; 11: 300-304, but only > in 15 micron intestine sections, so I do not know how it will > transpose to brain studies and the thicker sections > > Ronnie Houston > Anatomic Pathology Manager > Nationwide Children's Hospital > Columbus OH 43205 > (614) 722 5450 > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of > szi...@bio.u-szeged.hu > Sent: Wednesday, October 06, 2010 3:34 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] AChE fiber staining protocoll? > > Dear Histonet Members! > > We are going to investigate AChE fiber density changes in the cortex > of transcardially perfused rat brain slices (30 micrometer, criostat > sections, perfusing solution: 4% formaldehyde in PB ph 7.4). We need a > protocoll to visualize FINE fiber structure to be able to count changes. > > We used modification of Hedreen and original Tago protocoll. Hedreen > good results for general, but no fiber staining, Tago no sucsses. > > Could you please tell us some advise, protocolls functioning on teh > above mentioned objects? > > Thank you all in advance... > Csaba Szigeti > > ---------------------------------------------------------------- > This message was sent using IMP, the Internet Messaging Program. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ----------------------------------------- Confidentiality Notice: > The following mail message, including any attachments, is for the > sole use of the intended recipient(s) and may contain confidential > and privileged information. The recipient is responsible to > maintain the confidentiality of this information and to use the > information only for authorized purposes. If you are not the > intended recipient (or authorized to receive information for the > intended recipient), you are hereby notified that any review, use, > disclosure, distribution, copying, printing, or action taken in > reliance on the contents of this e-mail is strictly prohibited. If > you have received this communication in error, please notify us > immediately by reply e-mail and destroy all copies of the original > message. Thank you. > ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. ------------------------------ Message: 3 Date: Wed, 6 Oct 2010 11:01:52 -0400 From: "Weems, Joyce" <jwe...@sjha.org> Subject: [Histonet] RE: Helicobacter pylori To: "Kathy M. Gorham" <km...@grh.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <92ad9b20a6c38c4587a9febe3a30e1640398777...@chexcms10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" We use what we call a modified Genta. That is a modified Steiner if done manually. Because we have the Artisan and DAKO didn't have a Genta kit, I tried using the Warthin Starry and adding the Alcian blue and H&E manually and it worked fine. So we have not renamed it a modified Warthin Starry! But the H. pylori are very visible as well as the mucin and the morphology. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, October 06, 2010 10:20 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Helicobacter pylori Good Morning Histo land. I would like to know what others are using for a special stain for Helicobacter pylori not IHC. Do you use a kit? From where? Make up your own? Procedure. Thanks you. You have always been so helpful. Kathy Gorham H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research & Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 4 Date: Wed, 6 Oct 2010 11:03:05 -0400 From: "Diana McCaig" <dmcc...@ckha.on.ca> Subject: [Histonet] PAS STAIN To: <histonet@lists.utsouthwestern.edu> Message-ID: <dcfd9e6a390e294aaf3a2561cd32e5c4139d8...@ckhamail1.ckha.on.ca> Content-Type: text/plain; charset="us-ascii" Hi We have been doing a PAS stain on the same control block and same reagent supplier for a long time. Yesterday when we ran the slides, they failed to stain We opened new bottles of Periodic Acid and Schiff's Reagent and recut fresh controls Still, we are unable to get any staining. Suggestions to help would be appreciated Diana ------------------------------ Message: 5 Date: Wed, 6 Oct 2010 10:04:55 -0500 From: "Biedermann, JoAnn" <jabiederm...@uams.edu> Subject: [Histonet] Histokinette 2000 To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Message-ID: <833890c7e1be584b926a2584e6b37adc6b03f...@mail2.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" I have a Leica Histokinette 2000 that needs one beaker too be usable. Does anyone know where I can buy one of these? IMEB, inc does not have them. Jo Ann Biedermann Research Assistant University of Arkansas for Medical Sciences Reynolds Institute on Aging 629 Jack Stephens Drive Room 3173 Mail Slot 807 Little Rock, AR 72205 Phone: 501-526-5803 FAX: 501-526-5830 jabiederm...@uams.edu<mailto:jabiederm...@uams.edu> Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. ------------------------------ Message: 6 Date: Wed, 6 Oct 2010 11:06:12 -0400 From: "Rathborne, Toni" <trathbo...@somerset-healthcare.com> Subject: RE: [Histonet] PAS STAIN To: "Diana McCaig" <dmcc...@ckha.on.ca>, <histonet@lists.utsouthwestern.edu> Message-ID: <e78340c766a5284d999f5f5891ddf89014474...@smcmail.somerset-healthcare.com> Content-Type: text/plain; charset="utf-8" Were the new bottles from the same lot? -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Diana McCaig Sent: Wednesday, October 06, 2010 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS STAIN Hi We have been doing a PAS stain on the same control block and same reagent supplier for a long time. Yesterday when we ran the slides, they failed to stain We opened new bottles of Periodic Acid and Schiff's Reagent and recut fresh controls Still, we are unable to get any staining. Suggestions to help would be appreciated Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ------------------------------ Message: 7 Date: Wed, 6 Oct 2010 09:22:12 -0600 From: "Joanne Clark" <jcl...@pcnm.com> Subject: [Histonet] Query Special Stainers To: <histonet@lists.utsouthwestern.edu> Message-ID: <0cda5e1e01301f4880a8a7a8bcbda39c01375...@mail.pcnm.com> Content-Type: text/plain; charset="us-ascii" Hi All, we have an old Ventana benchmark NexES 9.0 that we used to use for our IHC. We now have a different IHC platform that we use and would like to change the Ventana over to use for running our special stains. Does anyone use the Ventana for their specials and have any advice or comments about how it performs? Thanks! Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico ------------------------------ Message: 8 Date: Wed, 6 Oct 2010 11:27:59 -0400 From: Brandi Higgins <brandihigg...@gmail.com> Subject: Re: [Histonet] Helicobacter pylori To: "Kathy M. Gorham" <km...@grh.org>, histonet@lists.utsouthwestern.edu Message-ID: <aanlktim=jfxk+fh+vhs-zhw=ow9kilu0normu89w9...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hello, We use what is pretty much a diff quik stain, the stain is called QW and it is from poly scientific. The procedure is really easy. Deparaffinize, alcohols, methanol, water and then we do 15 slow dips in the QW2, 15 slow dips in the QW3, wash in water, 2 quick dips in alcohol and then dry in the oven, coverslip. Depending on your pathologist's preference you may need to adjust the dips in QW3 or the dips in the alcohol (which will decolorize slightly). Hope this helps! Brandi Higgins, BS, HT(ASCP) On Wed, Oct 6, 2010 at 10:19 AM, Kathy M. Gorham <km...@grh.org> wrote: > Good Morning Histo land. I would like to know what others are using for > a special stain for Helicobacter pylori not IHC. Do you use a kit? From > where? Make up your own? Procedure. Thanks you. You have always been > so helpful. Kathy Gorham H.T. > > > GRH National Recognition > Outstanding Rural Health Organization of 2009 awarded by NRHA > Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP > Leader in Innovative Excellence 2009 awarded by the OAHHS > Financial Excellence Award 2010 awarded by the national Rural Health > Research & Policy Analysis Center > Healthcare Achievement Award for Quality in Patient Care Delivery and > Satisfaction 2010 awarded by Amerinet > > GRH Mission > We will ensure access to high-quality, cost-effective health services in a > safe, customer-friendly environment for all those in need of our services. > > > GRH Confidentiality Notice > This e-mail and any attached documents are for the intended recipient/s > only > and should be protected against viewing by unauthorized persons. The > information > herein may have been disclosed from records whose confidentiality is > protected > by Federal and State Law. Federal regulations prohibit further distribution > or > copying of this information without permission. If you received this > e-mail > transmission in error, please notify the sender immediately to arrange for > return > or destruction of this information. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 9 Date: Wed, 6 Oct 2010 17:39:03 +0200 From: "Gudrun Lang" <gu.l...@gmx.at> Subject: AW: [Histonet] PAS STAIN To: "'Diana McCaig'" <dmcc...@ckha.on.ca> Cc: histonet@lists.utsouthwestern.edu Message-ID: <986b2c6b449344ebaeceb4080a6e5...@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" You can test the activity of the Schiffs. Add a few drops of formalin to a small amount of Schiffs. There should be the typical pink stain. The pH should be about 2. If there are white flakes in the bottle, this could be a sign for a too high pH. Is your periodic acid ok? Gudrun -----Ursprüngliche Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Diana McCaig Gesendet: Mittwoch, 06. Oktober 2010 17:03 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] PAS STAIN Hi We have been doing a PAS stain on the same control block and same reagent supplier for a long time. Yesterday when we ran the slides, they failed to stain We opened new bottles of Periodic Acid and Schiff's Reagent and recut fresh controls Still, we are unable to get any staining. Suggestions to help would be appreciated Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Wed, 6 Oct 2010 09:40:17 -0600 From: "gayle callis" <gayle.cal...@bresnan.net> Subject: Where to buy Michels RE: [Histonet] DIF Transport Media To: "'Tony Henwood'" <antho...@chw.edu.au>, "'Della Speranza, Vinnie'" <del...@musc.edu>, "'kristen arvidson'" <arvidsonkris...@yahoo.com>, "'histonet'" <histonet@lists.utsouthwestern.edu> Message-ID: <002801cb656c$c8bd8cd0$5a38a6...@callis@bresnan.net> Content-Type: text/plain; charset="iso-8859-1" Dear Tony, Thank you for the fine reply on Michel's Transport Media. We had excellent results with human renal biopsies destined for immunofluorescence staining. I don't recall ever exceeding 48 hours in Michels as 72 hours was allowed per recommendation from the original and Elias's publications. The kidney morphology didn't suffer excessively from this transport media as seen on our H&E stained frozen sections from Liquid Nitrogen cooled isopentane snap frozen needle biopsies. My renal pathologist always commented that the frozen section H&E looked very much like his the FFPE H&E section. This must vary from laboratory to laboratory and also what tissue was transported e.g. skin biopsies versus kidney biopsies. We always were very careful with good Michels buffer rinses. As for where to access Michels Transport Media and Michels buffer, we purchased these from Poly Scientific in much larger volumes at a cheaper price than Wampole(?)(Zeus). Aliquots were made and distributed to laboratories although that may not be ideal since busy laboratories may find this a bit labor intensive. I know of laboratories who make up Michels with great success. As for transporting tissue on saline soaked gauze, I can't stress the importance enough to NEVER let the tissue dry out, and snap freeze it as soon as it arrives in the laboratory. I would prefer receiving a tissue in cell culture fluid to ensure no drying. The reference is much appreciated. G'day and missed seeing you at NSH symposium/convention this year. Gayle M. Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Tuesday, October 05, 2010 5:44 PM To: Della Speranza, Vinnie; kristen arvidson; histonet Subject: RE: [Histonet] DIF Transport Media Vinnie, I hope you are well. The following might be of use: Specimens for immunofluorescence are usually submitted to the laboratory in cell culture fluid (eg Hanks or RPMI) or on saline soaked gauze. For transport to other institutions, Michel's Transport Medium has often been used: Michel's Buffer 1M potassium citrate, pH 7.0 2.5 ml 0.1M magnesium sulphate 5.0 ml 0.1M N ethylmalemide 5.0 ml Distilled H2O 87.5 ml * Mix well and store at 2 8oC. Exp. 1 year Michel's Transport Medium Michel's Buffer 100ml Ammonium sulphate 55gm Adjust pH to 7.0 7.4 with 1M KOH. Store at 2 8oC. Exp. 1 year Unfortunately Michel's Transport Medium has erroneously been called Michel's Fixative. None of the components of Michel's Transport Medium is a fixative. Ammonium sulphate precipitates antigen-antibody complexes in diseased skin and renal tissues. N ethylmalemide modifies free sulphydryl groups of cysteine residues in proteins (Fischer 2006). Michel's Transport Medium has been shown to be deleterious to morphology. Ultrastructurally, complete destruction of plasma membranes and intracytoplasmic organelles occurs after 48 hours storage. On the other hand, antigenicity is well preserved even after many days storage (Fischer 2006). Fischer (2006) Intern J Surg Pathol 14(1):108. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Wednesday, 6 October 2010 6:10 AM To: 'kristen arvidson'; histonet Subject: RE: [Histonet] DIF Transport Media Zeus was a company that used to market michel's under their own label. I don't believe zeus still exists. Michel's is the name associated with the author of the original paper. I don't have the reference. This solution does not require refrigeration. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Monday, October 04, 2010 2:30 PM To: histonet Subject: [Histonet] DIF Transport Media Is Michel Medium the same as Zeus? Do these need to be refrigerated? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** ***** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. **************************************************************************** ***** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 5506 (20101005) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5506 (20101005) __________ The message was checked by ESET Smart Security. http://www.eset.com ------------------------------ Message: 11 Date: Wed, 6 Oct 2010 10:49:22 -0500 From: "Mahoney,Janice A" <janice.maho...@alegent.org> Subject: [Histonet] RE: Query Special Stainers To: 'Joanne Clark' <jcl...@pcnm.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <8f0ee4144e8e2f4ca1f6b051a2e5bfee029ca43...@exchmbc2.ad.ah.local> Content-Type: text/plain; charset="us-ascii" We have 2 Ventana special stainers and they are very reliable work horses. They run every day over half the day and put out very consistent results. Very user friendly too. Jan M Omaha -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne Clark Sent: Wednesday, October 06, 2010 10:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Query Special Stainers Hi All, we have an old Ventana benchmark NexES 9.0 that we used to use for our IHC. We now have a different IHC platform that we use and would like to change the Ventana over to use for running our special stains. Does anyone use the Ventana for their specials and have any advice or comments about how it performs? Thanks! Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ------------------------------ Message: 12 Date: Wed, 6 Oct 2010 10:04:44 -0600 From: "gayle callis" <gayle.cal...@bresnan.net> Subject: RE: [Histonet] PAS STAIN To: "'Rathborne, Toni'" <trathbo...@somerset-healthcare.com>, "'Diana McCaig'" <dmcc...@ckha.on.ca>, <histonet@lists.utsouthwestern.edu> Message-ID: <003401cb6570$33037c00$990a74...@callis@bresnan.net> Content-Type: text/plain; charset="utf-8" I suspect bad Periodic acid. We never buy periodic acid already in solution and if that has been sitting around even in a kit, it may have gone bad. In a workshop taught by Charles Culling, an expert on PAS staining, he stressed making periodic acid fresh daily or each time the stain is done. It is not expensive, goes into solution readily, then toss the periodic acid to never be reused with exception of that one day. Also, you can test your Schiffs reagent by putting a few drops of Schiffs into 10 mls NBF, watch it turn a very bright pink red instantly. If it turns purplish, it is not good. It may be a bad kit, but you never said you were using a kit only "new bottles of ....." Consequently we buy periodic acid in crystalline form, make up 1% Periodic acid, and buy the Schiffs Reagent from Fisher, never a kit. Sigma also sells good Schiffs, but Fisher Scientific aka Thermo has larger quantity for less price. We also store our Schiffs in the refrigerator rather than room temperature, a habit left over from days when we made this reagent up in house. Gayle M. Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, October 06, 2010 9:06 AM To: Diana McCaig; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PAS STAIN Were the new bottles from the same lot? -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Diana McCaig Sent: Wednesday, October 06, 2010 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS STAIN Hi We have been doing a PAS stain on the same control block and same reagent supplier for a long time. Yesterday when we ran the slides, they failed to stain We opened new bottles of Periodic Acid and Schiff's Reagent and recut fresh controls Still, we are unable to get any staining. Suggestions to help would be appreciated Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET Smart Security, version of virus signature database 5506 (20101005) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5506 (20101005) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5510 (20101006) __________ The message was checked by ESET Smart Security. http://www.eset.com __________ Information from ESET Smart Security, version of virus signature database 5510 (20101006) __________ The message was checked by ESET Smart Security. http://www.eset.com ------------------------------ Message: 13 Date: Wed, 06 Oct 2010 12:08:04 -0400 From: "Marcia Funk" <fu...@mercyhealth.com> Subject: [Histonet] RE: Query Special Stainers To: "Janice A Mahoney" <janice.maho...@alegent.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, "'Joanne Clark'" <jcl...@pcnm.com> Message-ID: <4cac5893.9b87.00a...@mercyhealth.com> Content-Type: text/plain; charset=US-ASCII We also have 2 Vantnan Special stainers and totally agree. Marcia M mason City Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-422-7907 >>> "Mahoney,Janice A" <janice.maho...@alegent.org> 10/06/2010 10:49 AM >>> We have 2 Ventana special stainers and they are very reliable work horses. They run every day over half the day and put out very consistent results. Very user friendly too. Jan M Omaha -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne Clark Sent: Wednesday, October 06, 2010 10:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Query Special Stainers Hi All, we have an old Ventana benchmark NexES 9.0 that we used to use for our IHC. We now have a different IHC platform that we use and would like to change the Ventana over to use for running our special stains. Does anyone use the Ventana for their specials and have any advice or comments about how it performs? Thanks! Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. 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Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Wed, 6 Oct 2010 14:13:23 -0230 From: Janet Keeping <jankeep...@gmail.com> Subject: [Histonet] PAS staining To: histonet@lists.utsouthwestern.edu Message-ID: <aanlktikwzc9t3oc4txtmomnbjy-n=vea_q+kootpw...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 I have for some time had a problem with Schiff's reagent and PAS staining. - I have tested each new, unopened bottle of Schiff's reagent with formaldehyde and always the color development was immediate, but purple, definately not pink.This result has been quite consistant. - PAS staining for glycogen using the method in *Histotechnology a self Instructional text,* would fail to demonstrate any glycogen in autopsy liver specimens. I teach Histology at a community college and this problem has driven me crazy for a number of years. I have tried several brands of Schiff with the same results. Recently I obtained sheep tissues which were promptly refrigerated and fixed after death, and I had hoped these tissues would demonstrate glycogen. ( My thinking was that perhaps delay before autopsy was somehow diminishing the glycogen in the specimens that I had.or that perhaps long term NBF fixation had hampered staining.) Basement membranes were stained with the Schiff reagent as expected despite the purple color in the formaldehyde test. Hotchkiss Mcmanus with the same reagent also produced beautiful staining of fungus a lovely magenta color. A search of the web made me suspiciious when I noted that Schiff added to 37-40% formaldehyde should produce a pink or red color, however, A spot check of formalin using Schiff should produce a purple color. I sent an e-mail to Brian Hewlett to ask if he could make any recommendation. Brian was not surprised by the purple color devopment in testing, He suggested that a large number of available aldehyde groups would be expected to produce this color. He suggested that I increase my periodate oxidation to 20-30 minutes and my Schiff application to 30 minutes. This worked and I am extremely grateful!. Has anyone else had an experience like this? Janet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 83, Issue 6 *************************************** To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. 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