Are you placing this bone section on a pre-cut control slides? The section will adhere better on its own slide.
Are you drying your slides at room temp prior to baking? It always helps to remove all trapped water from under the section prior to baking. Post fixing with formalin fumes is said to help. Remove slides from oven. Place in a rack in a staining cup w formalin just covering the bottom of the cup (not covering the section). Cover and allow the fumes to 'post fix' for 15 minutes. -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Vanessa J. Phelan Sent: Thursday, October 07, 2010 3:14 PM To: [email protected] Subject: [Histonet] Bone IHC Hi everyone, I was wondering if anyone has any tricks on how to get bone sections to stop lifting off the slide through the IHC process? I leave them in the oven for quite a while to make sure they are baked on, however after antigen retrieval (pressure cooker for 20mins) most of the boney part of the tissue comes off and the marrow and muscle stays put! The sections are cut onto superfrost plus slides. Any help would be much appreciated, thanks. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
