> You and your students are invited to attend the 3rd Advanced > Bio-Imaging Workshop at the University of Western Sydney on 16 - 19 > November, 2010 and the Bio-Imaging Expo (free event on 16th November > 2010) > > Tracking Molecules with Light > > Training in confocal imaging and protein 3D tracking, aggregation, > diffusion analyses. Experiments will involve mammalian cell lines, > invertebrate (coral) cells, plant and algae, bacteria and other > samples. > > Registration at www.uws.edu.au/3rd_advanced_bio-imaging_workshop. > > Places limited to 35 and only 20 places left so register now. > > > Location: Confocal Bio-Imaging Facility, Building S8, Hawkesbury > Campus, University of Western Sydney > Organiser: Dr Anya Salih > Training by: Dr Salih UWS; Prof. E. Gratton and Dr M. Digman Univ. > California; Prof. Guy Cox Uni Sydney; Dr Wolfgang Becker, Becker & > Hickl GmbH Germany; Dr C Thoni Leica Microsystems, G. Symonds Zeiss) > > > Lectures and intensive hands-on training on confocal microscopes by > top researchers in the field. Learn how to explore and analyse the 3D > structural complexity of invertebrate, animal & plant cells, tissues > and micro-organisms, visualize and analyse movement of organelles and > molecules. Trial a range of novel GFP-type protein constructs. Discuss > your experiments and trial new approaches. Workshop emphasis on > advanced confocal imaging techniques - FRET, FRAP, FCS, RICS, N&B, > FLIM, photoactivatable fluorescent proteins. > > Invited speakers > > * Professor Enrico Gratton, Director Laboratory for Fluorescence > Dynamics, University of California, Irvine > * Professor Takeharu Nagai, Laboratory for Nanosystems > Physiology & Nikon Imaging Center, Hokkaido University Research > Institute Electronic Science, Japan > * Dr. Michelle Digman, Director Optical Biology Core Facility, > University of California, Irvine > * A/Professor Guy Cox, Australian Centre for Microscopy & > Microanalysis, University of Sydney > * Professor Leann Tilley, Department of Biochemistry, D/Director > Centre of Excellence in coherent X-ray Science, La Trobe University > * Dr Will Hughes, Director, Pieter Huveneers Molecular Imaging > Facility, Garvan Institute of Medical Research, University of Sydney > * Dr Louise Cole, Advanced Microscopy Facility, Bosch Institute, > University of Sydney > * Dr Wolfgang Becker, Director Becker & Hickl GmbH, Berlin > > Training sessions cover the following: > > Multi-colour Fluorescent proteins > Genetically encodable GFP-type proteins (EGFP, YFP, CFP, mRuby, > pmKate2 from Evrogen), fused to studied proteins (mitochondrial, H2B > histone, actin, tubulin, Golgi, membrane); novel Photoactive > Fluorescent Proteins (EosFP, AmilRFP, kindling proteins, Phamret) from > reef corals. Biosensors - HypPer (Evrogen), Ca2+. Studies of protein > localization & diffusion. > > Confocal Spectral Imaging > Acquisition of microspectral data (x, y, lambda) in 3D image stacks > from samples with multiple fluorescent probes or from fluorescent > coral tissues expressing a variety of GFP-type proteins (A. Salih > fluorescent corals > http://www.abc.net.au/science/articles/2010/08/16/2984168.htm?topic=he > alth)- Spectral unmixing, analysis, spectral FRET. > > Analysis of molecular movement and diffusion > Track proteins and other molecules in live cells. Measure protein > femtoliter concentrations. Monitor mobility and binding using > fluorescence correlation spectroscopy (FCS), scanning FCS, raster > image correlation spectroscopy (RICS), number & brightness (N&B) and > photon counting histogram (PCH). > > Fluorescence Lifetime Imaging Microscopy (FLIM) > - a powerful tool to analyse spatial distribution of excited state > lifetimes in samples: studied examples will include FRET-FLIM to study > protein interactions (e.g., DNA-Protein) in cells, imaging of > photoactivatable FPs, quenching of chlorophyll in plants, etc. > Hands-on training in FLIM and Phasor FLIM. > > > Workshop microscopes & companies > > Leica TCS SP5 (two systems) - UV, VIS and IR in one system, acousto > optical beam splitter (AOBS), spectral imaging, FLIM, FCS, RICS, N&B > Zeiss LSM 780 confocal - 32-channel GaAsP array, spectral imaging, > photon counting, FCS Nikon A1, Coherent Scientific Pty. Ltd - rapid > image acquisition, resonant scanner & 32 channel microspectral > detection Olymus FluoView FV1000 - variable barrier filter (VBF), > spectral detection, RICS and N&B > Ultra VIEW VoX spinning disc confocal microscope, high speed, > multichannel, 2D and 3D, FRAP, FLIP, photoactivation experiments, > PerkinElmer. > Confocal FLIM system, Becker & Hickl GmbH, Berlin A range of new > GFP-type protein constructs of many colours (cyan to far red) linked > to a variety of cellular proteins for in vivo protein localization > and dynamic studies, Evrogen And many more other instruments. > > > Registration > > Full workshop registration (lectures + training) will be limited to 35 > participants. > Registration will be on a first come first served basis. > > Contact Anya Salih a.sa...@uws.edu.au to reserve your workshop place > Students $650 (GST inclusive) > All other $850 (GST inclusive) > > > Attendance of the BioImaging Expo on 16th November does not require > registration by please rsvp Pamela McMurtry > [pamela.mcmur...@theconferenceteam.com.au] > Accommodation details at registration website, from $260 per week per > single room at UWS College. > Bus shuttle will be available between Sydney airport and the workshop > venue at UWS campus. > > Kind regards, Anya
Dr Anya Salih Confocal Bio-Imaging Facility University Western Sydney Australia > 61 2 45701452 > a.sa...@uws.edu.au _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet