1% hydrochloric acid will eliminate the hematoxylin. You do not need to repeat HIER (if the section has already gone throught it). René J.
--- On Wed, 10/13/10, Margaryan, Naira <nmargar...@childrensmemorial.org> wrote: From: Margaryan, Naira <nmargar...@childrensmemorial.org> Subject: [Histonet] FW: How to remove Hematoxilin To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Date: Wednesday, October 13, 2010, 5:03 PM Hi histoworld, I would like to repeat my staining on the slides already coverslipped but need to remove hematoxilin first. How to remove hematoxilin? Is it need to repeat Antigen retrieval? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
