Dear Histo-netters, I am trying to work up a phosphor-akt antibody (Cell Signaling #3787) for my FFPE blocks of mouse uterine tubules. Fixation is in Z-fix for 48 hours. So far I have tried HIER with both microwave retrieval and a pressure-cooker. I am using DAKO's En-vision plus rabbit secondary HRP conjugate and reacting with DAB. The results are less than impressive, although I am seeing some low level staining at 1:50. Has anyone used this antibody with better success?
Thanks for any help in this matter. Katherine Walters Histology Director Central Microscopy Research Facilities 85 Eckstein Medical Research Building University of Iowa Iowa City, Iowa 52242-1101 phone: # 319-335-8142 fax: # 319-384-4469 katherine-walt...@uiowa.edu www.uiowa.edu/~cemrf _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
