Absolutely!! After the buffer rinse (after the counterstain), rinse in water, then 95, then 100, then xylene, and coverslip away!! I do it everyday and it works fine =)
Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-2907 -------- Original Message -------- Subject: [Histonet] Fwd: IHC coverglassing From: Jen Campbell <jcampbell_...@frontier.com> Date: Tue, October 19, 2010 10:16 am To: histonet@lists.utsouthwestern.edu Jen Campbell Muhlbauer Dermatopathology Laboratory Supervisor of Technical Services Phone 585-586-5166 Fax 585-586-3137 ----- Forwarded Message ----- From: "Jen Campbell" <jcampbell_...@frontier.com> To: "histonet" <histonet-boun...@lists.utsouthwestern.edu> Sent: Tuesday, October 19, 2010 11:16:38 AM Subject: IHC coverglassing Greetings to all the histonet followers!! A question was recently brought forth in the lab I work at. Can "non-automated" IHC slides be dehydrated and cleared(xylene substitute) after staining and be placed on an automated coverglasser? Jen Campbell Muhlbauer Dermatopathology Laboratory Supervisor of Technical Services Phone 585-586-5166 Fax 585-586-3137 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet