We put eosin in the first 100% alcohol. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Friday, October 22, 2010 11:18 AM To: Tench, Bill Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] eosin
It sounds like I am in the minority in pitting the eosin in the *first* 100% alcohol! I agree with Bill, it really makes a HUGE difference in small, minute tissues. We did run into one issue though, we were using orange biopsy cassettes and the red-orange tissue was difficult to spot in the orange cassettes! We have since switched to an aqua color and no more hiding specimens! ;o) Michelle On Oct 22, 2010, at 10:51 AM, "Tench, Bill" <bill.te...@pph.org> wrote: > I think the use in a processor becomes a local culture. I think > everyone in my community does it (someone spread the word), so now we > all do. I believe that we are all putting it in the last alcohol. It > really makes facing a block on minute pieces a whole lot easier. We > found marking the tissue directly tedious, and it didn't persist as well > in the processing. > Because there is often so little visible material in our FNA cell > blocks, we mark the histogel pellet with Davidson ink, and when the tech > has just faced off the ink, he/she knows its time to start collecting > sections. > > Bill Tench > Associate Dir. Laboratory Services > Chief, Cytology Services > Palomar Medical Center > 555 E. Valley Parkway > Escondido, California 92025 > bill.te...@pph.org > Voice: 760- 739-3037 > Fax: 760-739-2604 > > > [None] made the following annotations > --------------------------------------------------------------------- > Confidential E-Mail: This e-mail is intended only for the person or entity to > which it is addressed, and may contain information that is privileged, > confidential, or otherwise protected from disclosure. Dissemination, > distribution, or copying of this e-mail or the information herein by anyone > other than the intended recipient is prohibited. If you have received this > e-mail in error, please notify the sender by reply e-mail, and destroy the > original message and all copies. > --------------------------------------------------------------------- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
